Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018961938> ?p ?o ?g. }
- W2018961938 endingPage "757" @default.
- W2018961938 startingPage "751" @default.
- W2018961938 abstract "The objective of this study was to investigate the efficacy of combination gene therapy with multiple angiogenic growth factor cDNAs to enhance survival of ischemic skin flaps in a rat model. Sixty Sprague-Dawley rats were divided into six groups. Varying combinations of VEGF165, PDGF-B, and bFGF-plasmids were injected to prefabricate the flaps. Random skin flaps were raised on the dorsal aspect of rats following prefabrication with growth factor cDNAs. Flap viability was determined by measurement of percentage area of survival. The efficacy of gene therapy was evaluated by flap survival and neovascularization of representative histologic sections stained immunohistologically. The VEGF165 plus bFGF cDNAs enhanced the viability of the flap and neovascularization most effectively; the flap survival area was 64.3 ± 8.7% after transfer of these two growth factor genes. Addition of PDGF-B cDNA is deleterious to the effects of combined VEGF165 and bFGF, leading to a significant decrease in flap viability (44.9 ± 2.7%). Viability of the flaps with combined VEGF165 and bFGF cDNA transfer was significantly greater than that of the flaps with VEGF165 transfer alone (57.6 ± 5.2%) or sham plasmid control (52.3 ± 5.0%). Combined transfer of VEGF165 and bFGF cDNA is the most effective combination of multiple growth factor genes to improve flap viability in this model. Simultaneous transfer of three growth factor genes (VEGF165, PDGF-B, and bFGF) is deleterious to flap survival, at least for the ratio of lipofectin:transgene employed." @default.
- W2018961938 created "2016-06-24" @default.
- W2018961938 creator A5006546115 @default.
- W2018961938 creator A5015201898 @default.
- W2018961938 creator A5042263495 @default.
- W2018961938 creator A5048803866 @default.
- W2018961938 creator A5065444755 @default.
- W2018961938 creator A5083571909 @default.
- W2018961938 creator A5088278158 @default.
- W2018961938 date "2005-11-01" @default.
- W2018961938 modified "2023-09-26" @default.
- W2018961938 title "Efficacy of Combination Gene Therapy with Multiple Growth Factor cDNAs to Enhance Skin Flap Survival in a Rat Model" @default.
- W2018961938 cites W1965560753 @default.
- W2018961938 cites W1973515582 @default.
- W2018961938 cites W1982384071 @default.
- W2018961938 cites W1988596206 @default.
- W2018961938 cites W1988978946 @default.
- W2018961938 cites W1994399017 @default.
- W2018961938 cites W1998650818 @default.
- W2018961938 cites W1998710824 @default.
- W2018961938 cites W1999360913 @default.
- W2018961938 cites W2002880608 @default.
- W2018961938 cites W2005333814 @default.
- W2018961938 cites W2011214284 @default.
- W2018961938 cites W2020187956 @default.
- W2018961938 cites W2024186814 @default.
- W2018961938 cites W2026151249 @default.
- W2018961938 cites W2027897487 @default.
- W2018961938 cites W2033655249 @default.
- W2018961938 cites W2039565133 @default.
- W2018961938 cites W2048525391 @default.
- W2018961938 cites W2054731255 @default.
- W2018961938 cites W2062281431 @default.
- W2018961938 cites W2065690467 @default.
- W2018961938 cites W2068176955 @default.
- W2018961938 cites W2068269898 @default.
- W2018961938 cites W2070522073 @default.
- W2018961938 cites W2080505451 @default.
- W2018961938 cites W2087650119 @default.
- W2018961938 cites W2103264551 @default.
- W2018961938 cites W2126419751 @default.
- W2018961938 cites W2164998479 @default.
- W2018961938 cites W2313040660 @default.
- W2018961938 cites W2333001793 @default.
- W2018961938 cites W4255366085 @default.
- W2018961938 doi "https://doi.org/10.1089/dna.2005.24.751" @default.
- W2018961938 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16274295" @default.
- W2018961938 hasPublicationYear "2005" @default.
- W2018961938 type Work @default.
- W2018961938 sameAs 2018961938 @default.
- W2018961938 citedByCount "22" @default.
- W2018961938 countsByYear W20189619382014 @default.
- W2018961938 countsByYear W20189619382015 @default.
- W2018961938 countsByYear W20189619382017 @default.
- W2018961938 countsByYear W20189619382018 @default.
- W2018961938 countsByYear W20189619382021 @default.
- W2018961938 countsByYear W20189619382022 @default.
- W2018961938 crossrefType "journal-article" @default.
- W2018961938 hasAuthorship W2018961938A5006546115 @default.
- W2018961938 hasAuthorship W2018961938A5015201898 @default.
- W2018961938 hasAuthorship W2018961938A5042263495 @default.
- W2018961938 hasAuthorship W2018961938A5048803866 @default.
- W2018961938 hasAuthorship W2018961938A5065444755 @default.
- W2018961938 hasAuthorship W2018961938A5083571909 @default.
- W2018961938 hasAuthorship W2018961938A5088278158 @default.
- W2018961938 hasConcept C102230213 @default.
- W2018961938 hasConcept C104317684 @default.
- W2018961938 hasConcept C111599444 @default.
- W2018961938 hasConcept C146285616 @default.
- W2018961938 hasConcept C1491633281 @default.
- W2018961938 hasConcept C153911025 @default.
- W2018961938 hasConcept C167734588 @default.
- W2018961938 hasConcept C170493617 @default.
- W2018961938 hasConcept C180361614 @default.
- W2018961938 hasConcept C187882448 @default.
- W2018961938 hasConcept C2775960820 @default.
- W2018961938 hasConcept C2777025900 @default.
- W2018961938 hasConcept C2777587049 @default.
- W2018961938 hasConcept C2778271429 @default.
- W2018961938 hasConcept C2780394083 @default.
- W2018961938 hasConcept C2781294074 @default.
- W2018961938 hasConcept C502942594 @default.
- W2018961938 hasConcept C53227056 @default.
- W2018961938 hasConcept C55493867 @default.
- W2018961938 hasConcept C86803240 @default.
- W2018961938 hasConceptScore W2018961938C102230213 @default.
- W2018961938 hasConceptScore W2018961938C104317684 @default.
- W2018961938 hasConceptScore W2018961938C111599444 @default.
- W2018961938 hasConceptScore W2018961938C146285616 @default.
- W2018961938 hasConceptScore W2018961938C1491633281 @default.
- W2018961938 hasConceptScore W2018961938C153911025 @default.
- W2018961938 hasConceptScore W2018961938C167734588 @default.
- W2018961938 hasConceptScore W2018961938C170493617 @default.
- W2018961938 hasConceptScore W2018961938C180361614 @default.
- W2018961938 hasConceptScore W2018961938C187882448 @default.
- W2018961938 hasConceptScore W2018961938C2775960820 @default.
- W2018961938 hasConceptScore W2018961938C2777025900 @default.
- W2018961938 hasConceptScore W2018961938C2777587049 @default.