FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018980750> ?p ?o ?g. }

• W2018980750 endingPage "295" @default.
• W2018980750 startingPage "290" @default.
• W2018980750 abstract "Abstract A 3-kbp DNA fragment including the l -2,3-butanediol dehydrogenase ( l -BDH) gene ( bud C) from the chromosomal DNA of Brevibacterium saccharolyticum C-1012 was cloned in Escherichia coli JM109 after its insertion into pBluescript II SK + , and the resulting plasmid was named pLBD-SK. The bud C had an open reading frame consisting of 774 bp and encoded 258 amino acids. It was not included in a 2,3-butanediol operon such as is seen in the case of the meso -BDH gene ( bud C) of Klebsiella pneumoniae . For the expression of the bud C, the deletion plasmid pLBD2-119 was prepared from pLBD-SK. E. coli JM109/pLBD2-119 had higher l -BDH activity than that of Br. saccharolyticum C-1012. The l -BDH appeared as two bands on disc-PAGE. Isopropyl-β- d -thiogalacto-pyranoside (IPTG) influenced the quantity radio of the electrophoretic isoenzymes of l -BDH from E. coli JM109/pLBD2-119 that is, a higher relative mobility band with weak substrate specificity was abundantly produced by IPTG. The BDH was considered to belong to the short-chain dehydrogenase/reductase (SDR) family on the basis of the following distinctive features: it possessed two conservative sequences GXXXGXG and YXXXK, and it consisted of about 250 amino acids. As a result of a phylogenetic analysis of SDR family enzymes, the BDHs were considered to comprise a cluster independent from the other SDR enzymes." @default.
• W2018980750 created "2016-06-24" @default.
• W2018980750 creator A5001005996 @default.
• W2018980750 creator A5019809723 @default.
• W2018980750 creator A5022353603 @default.
• W2018980750 creator A5025180140 @default.
• W2018980750 creator A5038195855 @default.
• W2018980750 creator A5041482552 @default.
• W2018980750 creator A5052831124 @default.
• W2018980750 date "1998-01-01" @default.
• W2018980750 modified "2023-09-25" @default.
• W2018980750 title "Cloning, expression and nucleotide sequence of the l-2,3-butanediol dehydrogenase gene from Brevibacterium saccharolyticum C-1012" @default.
• W2018980750 cites W1502162512 @default.
• W2018980750 cites W1849836964 @default.
• W2018980750 cites W1969337918 @default.
• W2018980750 cites W1997585861 @default.
• W2018980750 cites W2014770523 @default.
• W2018980750 cites W2019410656 @default.
• W2018980750 cites W2031906840 @default.
• W2018980750 cites W2045973786 @default.
• W2018980750 cites W2063450941 @default.
• W2018980750 cites W2070330177 @default.
• W2018980750 cites W2077156009 @default.
• W2018980750 cites W2111301002 @default.
• W2018980750 cites W2132068224 @default.
• W2018980750 cites W2328197679 @default.
• W2018980750 cites W4254275792 @default.
• W2018980750 doi "https://doi.org/10.1016/s0922-338x(98)80132-3" @default.
• W2018980750 hasPublicationYear "1998" @default.
• W2018980750 type Work @default.
• W2018980750 sameAs 2018980750 @default.
• W2018980750 citedByCount "22" @default.
• W2018980750 countsByYear W20189807502012 @default.
• W2018980750 countsByYear W20189807502013 @default.
• W2018980750 countsByYear W20189807502014 @default.
• W2018980750 countsByYear W20189807502015 @default.
• W2018980750 countsByYear W20189807502016 @default.
• W2018980750 countsByYear W20189807502020 @default.
• W2018980750 countsByYear W20189807502021 @default.
• W2018980750 crossrefType "journal-article" @default.
• W2018980750 hasAuthorship W2018980750A5001005996 @default.
• W2018980750 hasAuthorship W2018980750A5019809723 @default.
• W2018980750 hasAuthorship W2018980750A5022353603 @default.
• W2018980750 hasAuthorship W2018980750A5025180140 @default.
• W2018980750 hasAuthorship W2018980750A5038195855 @default.
• W2018980750 hasAuthorship W2018980750A5041482552 @default.
• W2018980750 hasAuthorship W2018980750A5052831124 @default.
• W2018980750 hasConcept C100544194 @default.
• W2018980750 hasConcept C104317684 @default.
• W2018980750 hasConcept C121050878 @default.
• W2018980750 hasConcept C150194340 @default.
• W2018980750 hasConcept C153911025 @default.
• W2018980750 hasConcept C175605896 @default.
• W2018980750 hasConcept C185592680 @default.
• W2018980750 hasConcept C19924922 @default.
• W2018980750 hasConcept C199360897 @default.
• W2018980750 hasConcept C2775991252 @default.
• W2018980750 hasConcept C2778386651 @default.
• W2018980750 hasConcept C41008148 @default.
• W2018980750 hasConcept C523546767 @default.
• W2018980750 hasConcept C54355233 @default.
• W2018980750 hasConcept C55493867 @default.
• W2018980750 hasConcept C84148353 @default.
• W2018980750 hasConcept C86803240 @default.
• W2018980750 hasConceptScore W2018980750C100544194 @default.
• W2018980750 hasConceptScore W2018980750C104317684 @default.
• W2018980750 hasConceptScore W2018980750C121050878 @default.
• W2018980750 hasConceptScore W2018980750C150194340 @default.
• W2018980750 hasConceptScore W2018980750C153911025 @default.
• W2018980750 hasConceptScore W2018980750C175605896 @default.
• W2018980750 hasConceptScore W2018980750C185592680 @default.
• W2018980750 hasConceptScore W2018980750C19924922 @default.
• W2018980750 hasConceptScore W2018980750C199360897 @default.
• W2018980750 hasConceptScore W2018980750C2775991252 @default.
• W2018980750 hasConceptScore W2018980750C2778386651 @default.
• W2018980750 hasConceptScore W2018980750C41008148 @default.
• W2018980750 hasConceptScore W2018980750C523546767 @default.
• W2018980750 hasConceptScore W2018980750C54355233 @default.
• W2018980750 hasConceptScore W2018980750C55493867 @default.
• W2018980750 hasConceptScore W2018980750C84148353 @default.
• W2018980750 hasConceptScore W2018980750C86803240 @default.
• W2018980750 hasIssue "3" @default.
• W2018980750 hasLocation W20189807501 @default.
• W2018980750 hasOpenAccess W2018980750 @default.
• W2018980750 hasPrimaryLocation W20189807501 @default.
• W2018980750 hasRelatedWork W2056172754 @default.
• W2018980750 hasRelatedWork W2349458063 @default.
• W2018980750 hasRelatedWork W2349475057 @default.
• W2018980750 hasRelatedWork W2355280460 @default.
• W2018980750 hasRelatedWork W2355730370 @default.
• W2018980750 hasRelatedWork W2360549342 @default.
• W2018980750 hasRelatedWork W2373528557 @default.
• W2018980750 hasRelatedWork W2387211330 @default.
• W2018980750 hasRelatedWork W2388615639 @default.
• W2018980750 hasRelatedWork W4243829279 @default.
• W2018980750 hasVolume "86" @default.
• W2018980750 isParatext "false" @default.
• W2018980750 isRetracted "false" @default.

• next