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- W2018995498 abstract "The initial interaction between a natural messenger RNA and ribosomes in a eukaryotic system was studied. Utilizing a population of 1.0 M KCl-washed ribosomes and isolated myosin mRNA, only the 40S subunits bound mRNA to form initiation complex I. This binding required the presence of initiation factor EF3 and either GTP or its analogue, GDPCP. When unfractionated initiation factors, tRNA and GTP were added to the reaction mixture, the 60S ribosomal subunit joined with complex I to produce complex II. Complex II could not be demonstrated in the absence of GTP or when GTP was replaced by its analogue, GDPCP." @default.
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- W2018995498 modified "2023-10-18" @default.
- W2018995498 title "Studies on the formation of the initiation complex in eukaryotes" @default.
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- W2018995498 doi "https://doi.org/10.1016/0006-291x(71)90637-1" @default.
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