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• W2018997277 abstract "Several years ago evidence for a so-called “unitary glutamate receptor” was published. This unique type of glutamate receptor was reported to be activated by the traditional agonists of all three major glutamate receptor subfamilies [i.e., α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), kainate, and <i>N</i>-methyl-d-aspartate (NMDA)] in a glycine-dependent as well as magnesium-blockable manner and was reported to consist of an NR1 subunit coexpressed with the kainate binding protein (KBP) from <i>Xenopus laevis</i>, <i>Xen</i>U1. To re-examine the existence of such a receptor, we cloned two splice variants of the <i>X. laevis</i> NMDA receptor subunit NR1, <i>Xen</i>NR1-4a and <i>Xen</i>NR1-4b, and expressed them in <i>X. laevis</i> oocytes as well as in human embryonic kidney (HEK) 293 cells, either alone or with the <i>X. laevis</i> KBP subunit <i>Xen</i>U1. In addition, we coexpressed <i>Xen</i>U1 separately with all eight splice variants of the rat NR1 subunit. In no case did we see evidence of a unitary glutamate receptor pharmacology. In HEK293 cells, we did not get receptor response unless an NR2 subunit was coexpressed. In <i>X. laevis</i> oocytes, we did observe responses to glutamate/glycine as well as small responses to glycine alone, but these were independent of coexpressed <i>Xen</i>U1. Neither AMPA nor kainate ever elicited significant responses. Because we verified that <i>Xen</i>U1 is expressed and inserted into the plasma membrane of HEK293 cells, we conclude that <i>Xen</i>U1 and NR1 do not form the postulated unitary glutamate receptor. Furthermore, successful amplification of a fragment of a <i>X. laevis</i> NR2 subunit indicates that <i>X. laevis</i> uses NR2 subunits and not <i>Xen</i>U1 to form heteromeric complexes with NR1." @default.
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• W2018997277 date "2005-10-07" @default.
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• W2018997277 title "Revisiting the Postulated “Unitary Glutamate Receptor”: Electrophysiological and Pharmacological Analysis in Two Heterologous Expression Systems Fails to Detect Evidence for Its Existence" @default.
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• W2018997277 doi "https://doi.org/10.1124/mol.105.016840" @default.
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