FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019019265> ?p ?o ?g. }

• W2019019265 endingPage "90" @default.
• W2019019265 startingPage "80" @default.
• W2019019265 abstract "Novel formulations based on physiologically occurring anionic lipids have been designed to achieve safe and efficient siRNA delivery. Anionic liposomes (DOPG/DOPE) were complexed with siRNA using calcium ion bridges to prepare anionic lipoplexes. Various formulation parameters (liposome composition, lipid and calcium concentration) were evaluated and optimized to achieve efficient silencing and high cell viability in breast cancer cells. The optimal anionic lipoplexes composed of 1 μg/mL lipid (40:60 (DOPG/DOPE m/m)), 2.4 mM calcium and 10 nM siRNA, showed maximum silencing (∼70% knockdown) without being cytotoxic. These lipoplexes also showed stability and high efficiency in the presence of serum. Additionally, optimal anionic lipoplexes showed efficient intracellular uptake and endosomal escape. Characterization studies indicated the optimal anionic formulations were 324.2 ± 19.6 nm with a surface charge of (−22.9 ± 0.1) mV and 98.5 ± 1.4% encapsulation efficiency. Control cationic lipoplexes (Lipofectamine 2000) showed silencing comparable to the anionic lipoplexes but were highly cytotoxic as indicated by IC50 values (cationic – 22.9 μg/mL, compared to anionic – greater than 107 μg/mL). Calcium–siRNA complexes (without liposomes) showed low efficiency (∼50% silencing), and highly variable results. The optimized anionic formulations may offer a safer alternative to conventional cationic based systems for efficient in vitro as well as in vivo delivery of therapeutic siRNAs." @default.
• W2019019265 created "2016-06-24" @default.
• W2019019265 creator A5000724815 @default.
• W2019019265 creator A5089510416 @default.
• W2019019265 date "2012-08-01" @default.
• W2019019265 modified "2023-10-13" @default.
• W2019019265 title "Efficient and safe delivery of siRNA using anionic lipids: Formulation optimization studies" @default.
• W2019019265 cites W1581889893 @default.
• W2019019265 cites W1881967720 @default.
• W2019019265 cites W1964090685 @default.
• W2019019265 cites W1968913592 @default.
• W2019019265 cites W1979993551 @default.
• W2019019265 cites W1993776299 @default.
• W2019019265 cites W1997519809 @default.
• W2019019265 cites W2000130544 @default.
• W2019019265 cites W2011675950 @default.
• W2019019265 cites W2013603367 @default.
• W2019019265 cites W2017961374 @default.
• W2019019265 cites W2021905258 @default.
• W2019019265 cites W2025418242 @default.
• W2019019265 cites W2034342858 @default.
• W2019019265 cites W2034413987 @default.
• W2019019265 cites W2046003078 @default.
• W2019019265 cites W2047834274 @default.
• W2019019265 cites W2054683516 @default.
• W2019019265 cites W2064100334 @default.
• W2019019265 cites W2065068060 @default.
• W2019019265 cites W2065907421 @default.
• W2019019265 cites W2068322824 @default.
• W2019019265 cites W2068422066 @default.
• W2019019265 cites W2068942685 @default.
• W2019019265 cites W2077112895 @default.
• W2019019265 cites W2079730270 @default.
• W2019019265 cites W2079902250 @default.
• W2019019265 cites W2080648192 @default.
• W2019019265 cites W2082890710 @default.
• W2019019265 cites W2087403976 @default.
• W2019019265 cites W2088181746 @default.
• W2019019265 cites W2089594948 @default.
• W2019019265 cites W2092770422 @default.
• W2019019265 cites W2095509700 @default.
• W2019019265 cites W2096040781 @default.
• W2019019265 cites W2099113192 @default.
• W2019019265 cites W2099871857 @default.
• W2019019265 cites W2104050632 @default.
• W2019019265 cites W2110435108 @default.
• W2019019265 cites W2111934381 @default.
• W2019019265 cites W2112932690 @default.
• W2019019265 cites W2116424844 @default.
• W2019019265 cites W2117117603 @default.
• W2019019265 cites W2125301080 @default.
• W2019019265 cites W2126020016 @default.
• W2019019265 cites W2131095976 @default.
• W2019019265 cites W2132741431 @default.
• W2019019265 cites W2147584970 @default.
• W2019019265 cites W2151902657 @default.
• W2019019265 cites W2323505647 @default.
• W2019019265 cites W4253747558 @default.
• W2019019265 doi "https://doi.org/10.1016/j.ijpharm.2012.04.058" @default.
• W2019019265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22575754" @default.
• W2019019265 hasPublicationYear "2012" @default.
• W2019019265 type Work @default.
• W2019019265 sameAs 2019019265 @default.
• W2019019265 citedByCount "50" @default.
• W2019019265 countsByYear W20190192652012 @default.
• W2019019265 countsByYear W20190192652013 @default.
• W2019019265 countsByYear W20190192652014 @default.
• W2019019265 countsByYear W20190192652015 @default.
• W2019019265 countsByYear W20190192652016 @default.
• W2019019265 countsByYear W20190192652017 @default.
• W2019019265 countsByYear W20190192652018 @default.
• W2019019265 countsByYear W20190192652019 @default.
• W2019019265 countsByYear W20190192652020 @default.
• W2019019265 countsByYear W20190192652021 @default.
• W2019019265 countsByYear W20190192652022 @default.
• W2019019265 countsByYear W20190192652023 @default.
• W2019019265 crossrefType "journal-article" @default.
• W2019019265 hasAuthorship W2019019265A5000724815 @default.
• W2019019265 hasAuthorship W2019019265A5089510416 @default.
• W2019019265 hasConcept C104317684 @default.
• W2019019265 hasConcept C119056186 @default.
• W2019019265 hasConcept C12554922 @default.
• W2019019265 hasConcept C155887181 @default.
• W2019019265 hasConcept C173396325 @default.
• W2019019265 hasConcept C178790620 @default.
• W2019019265 hasConcept C183882617 @default.
• W2019019265 hasConcept C185154212 @default.
• W2019019265 hasConcept C185592680 @default.
• W2019019265 hasConcept C190283241 @default.
• W2019019265 hasConcept C22615655 @default.
• W2019019265 hasConcept C519063684 @default.
• W2019019265 hasConcept C54009773 @default.
• W2019019265 hasConcept C55493867 @default.
• W2019019265 hasConcept C86803240 @default.
• W2019019265 hasConceptScore W2019019265C104317684 @default.
• W2019019265 hasConceptScore W2019019265C119056186 @default.
• W2019019265 hasConceptScore W2019019265C12554922 @default.
• W2019019265 hasConceptScore W2019019265C155887181 @default.

• next