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• W2019022944 abstract "<b>Objective</b> : Several medicinal plants have been evaluated for their antidiarrhoeal activity. Most studies evaluated their effect on intestinal motility and antimicrobial activity and, therefore, did not take into account the pathogenesis of infectious diarrhoea. Features of infectious diarrhoea like abdominal pain, cramps, inflammation, and passage of blood/mucus in the stools are the combined effect of one or more virulence factors of the infecting organism. The effect of medicinal plants on the microbial virulent features can serve as marker(s) for testing their efficacy. In this study, we evaluated the effect of a decoction of dried leaves of <i> Dalbergia sissoo</i> on aspects of pathogenicity, that is, colonisation to intestinal epithelial cells and production/action of enterotoxins. This was done to define its possible mechanism(s) of action in infectious diarrhoea.<br><b> Materials and Methods</b> : Antibacterial, antiprotozoal, and antiviral activities of the plant decoction were checked by agar dilution method, tube dilution method, and neutral red uptake assay, respectively. Cholera toxin (CT) and <i> Escherichia coli</i> labile toxin (LT) were assayed by ganglioside monosialic acid receptor ELISA. Suckling mouse assay was used to assess <i> E. coli</i> stable toxin (ST). As a measure of colonisation, the effect against adherence of <i> E. coli</i> and invasion of <i> E. coli</i> and <i> Shigella flexneri</i> to HEp-2 cells were studied.<i> <br></i> Results: The decoction had no antibacterial, antiprotozoal, and antiviral activity. It reduced the production and the binding of CT and bacterial adherence and invasion.<br><b> Conclusion</b> : This study showed that <i> D</i> . <i> sissoo</i> is antidiarrhoeal as it affects bacterial virulence. However, it has no antimicrobial activity." @default.
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• W2019022944 date "2006-01-01" @default.
• W2019022944 modified "2023-10-10" @default.
• W2019022944 title "Studies on<i>Dalbergia sissoo</i>(Roxb.)<i />leaves: Possible mechanism(s) of action in infectious diarrhoea" @default.
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• W2019022944 doi "https://doi.org/10.4103/0253-7613.24618" @default.
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