FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019039078> ?p ?o ?g. }

• W2019039078 abstract "Our knowledge of the molecules that interact with sperm at the egg membrane is restricted to a short list. In the eggs of Discoglossus pictus, fusion with sperm is limited to a differentiated structure, the dimple, offering several advantages for detecting molecules involved in fertilization. Previous studies have identified fucosylated glycoproteins of 200, 260, and 270 kDa located at the surface of the dimple that are able to bind sperm in vitro. Here, we show that dimple glycoproteins and a protein represented by a 120-kDa band released following gel-into-gel SDS-PAGE of both glycoproteins share the same N-terminal amino acid sequence, which itself is similar to the N-termini of Xenopus liver-synthesized vitellogenin (VTG) and the lipovitellin 1. MALDI/MS mass spectrometry indicated that the 120-kDa band is part of both gps 200 and 270/260. A 117-kDa major protein of the egg lysate exhibits the same MALDI/MS spectrum, and LC-MSMS indicates that this is a lipovitellin 1 (DpLIV) that coincides with the 120-kDa band and is responsible for the formation of the 200-270-kDa dimers. Therefore, lipovitellin 1 constitutes the protein backbone of the dimple glycoconjugates. In vitro assays using polystyrene beads coated with DpLIV or with its dimers indicate that significant sperm binding occurs only with DpLIV dimers. In amphibians, VTG is taken up by the oocyte, where it releases lipovitellins destined to form yolk. In Discoglossus, our data suggest that yolk proteins are also synthesized by the oocyte. The dimple forms in the ovulated oocyte following the exocytosis of vesicles that likely expose DpLIVs at their membrane. Indeed, in whole mounts of immunostained eggs, anti-vitellogenin antibodies label only the surface of the dimple." @default.
• W2019039078 created "2016-06-24" @default.
• W2019039078 creator A5014809000 @default.
• W2019039078 creator A5016377238 @default.
• W2019039078 creator A5032149067 @default.
• W2019039078 creator A5038167994 @default.
• W2019039078 creator A5047228372 @default.
• W2019039078 creator A5050578877 @default.
• W2019039078 creator A5052484166 @default.
• W2019039078 creator A5054589020 @default.
• W2019039078 creator A5055667657 @default.
• W2019039078 creator A5072047079 @default.
• W2019039078 creator A5079506550 @default.
• W2019039078 creator A5091838991 @default.
• W2019039078 creator A5008727079 @default.
• W2019039078 date "2011-02-09" @default.
• W2019039078 modified "2023-10-01" @default.
• W2019039078 title "Lipovitellin constitutes the protein backbone of glycoproteins involved in sperm-egg interaction in the amphibian Discoglossus pictus" @default.
• W2019039078 cites W1547464519 @default.
• W2019039078 cites W1977541160 @default.
• W2019039078 cites W1981830206 @default.
• W2019039078 cites W198344517 @default.
• W2019039078 cites W1988339417 @default.
• W2019039078 cites W1995194414 @default.
• W2019039078 cites W1996095223 @default.
• W2019039078 cites W1999404230 @default.
• W2019039078 cites W2001097916 @default.
• W2019039078 cites W2019225379 @default.
• W2019039078 cites W2019271142 @default.
• W2019039078 cites W2019554538 @default.
• W2019039078 cites W2021587657 @default.
• W2019039078 cites W2023599431 @default.
• W2019039078 cites W2023679907 @default.
• W2019039078 cites W2023759826 @default.
• W2019039078 cites W2027992714 @default.
• W2019039078 cites W2032010211 @default.
• W2019039078 cites W2038717014 @default.
• W2019039078 cites W2045017588 @default.
• W2019039078 cites W2045974132 @default.
• W2019039078 cites W2046055734 @default.
• W2019039078 cites W2057038198 @default.
• W2019039078 cites W2060497666 @default.
• W2019039078 cites W2061305688 @default.
• W2019039078 cites W2074336681 @default.
• W2019039078 cites W2075672556 @default.
• W2019039078 cites W2081136202 @default.
• W2019039078 cites W2085077505 @default.
• W2019039078 cites W2085682514 @default.
• W2019039078 cites W2087176961 @default.
• W2019039078 cites W2088381646 @default.
• W2019039078 cites W2089003364 @default.
• W2019039078 cites W2092692992 @default.
• W2019039078 cites W2112860304 @default.
• W2019039078 cites W2114150800 @default.
• W2019039078 cites W2116472416 @default.
• W2019039078 cites W2124820993 @default.
• W2019039078 cites W2129487015 @default.
• W2019039078 cites W2132679436 @default.
• W2019039078 cites W2137383680 @default.
• W2019039078 cites W2151119702 @default.
• W2019039078 cites W2160240715 @default.
• W2019039078 cites W2167074019 @default.
• W2019039078 cites W2336731832 @default.
• W2019039078 cites W4240334860 @default.
• W2019039078 doi "https://doi.org/10.1002/mrd.21282" @default.
• W2019039078 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21308852" @default.
• W2019039078 hasPublicationYear "2011" @default.
• W2019039078 type Work @default.
• W2019039078 sameAs 2019039078 @default.
• W2019039078 citedByCount "6" @default.
• W2019039078 countsByYear W20190390782012 @default.
• W2019039078 countsByYear W20190390782013 @default.
• W2019039078 countsByYear W20190390782014 @default.
• W2019039078 countsByYear W20190390782016 @default.
• W2019039078 countsByYear W20190390782018 @default.
• W2019039078 countsByYear W20190390782020 @default.
• W2019039078 crossrefType "journal-article" @default.
• W2019039078 hasAuthorship W2019039078A5008727079 @default.
• W2019039078 hasAuthorship W2019039078A5014809000 @default.
• W2019039078 hasAuthorship W2019039078A5016377238 @default.
• W2019039078 hasAuthorship W2019039078A5032149067 @default.
• W2019039078 hasAuthorship W2019039078A5038167994 @default.
• W2019039078 hasAuthorship W2019039078A5047228372 @default.
• W2019039078 hasAuthorship W2019039078A5050578877 @default.
• W2019039078 hasAuthorship W2019039078A5052484166 @default.
• W2019039078 hasAuthorship W2019039078A5054589020 @default.
• W2019039078 hasAuthorship W2019039078A5055667657 @default.
• W2019039078 hasAuthorship W2019039078A5072047079 @default.
• W2019039078 hasAuthorship W2019039078A5079506550 @default.
• W2019039078 hasAuthorship W2019039078A5091838991 @default.
• W2019039078 hasConcept C104317684 @default.
• W2019039078 hasConcept C105702510 @default.
• W2019039078 hasConcept C108625454 @default.
• W2019039078 hasConcept C18903297 @default.
• W2019039078 hasConcept C196843134 @default.
• W2019039078 hasConcept C2776690073 @default.
• W2019039078 hasConcept C2779078696 @default.
• W2019039078 hasConcept C2779535977 @default.
• W2019039078 hasConcept C2781087480 @default.
• W2019039078 hasConcept C2781321622 @default.

• next