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• W2019071427 abstract "We previously reported and partially characterized a unique monoclonal antibody (mAb), U5A2-13, which recognizes a T cell subset similar to NK1.1+ T cells, not only in NK1.1-positive mouse strains but also in NK1.1-negative strains. In NK1.1-positive C57BL/6 mice, U5A2-13+ TCRα β+ cells produced abundant IL-4 as well as extremely high levels of IFN-γ upon CD3 cross-linking, but this did not occur with U5A2-13– TCRα β+ cells. In NK1.1- negative C3H/He mice, U5A2-13+ TCRα β+ cells produced high levels of IL-4 and IFN-γ upon CD3 cross-linking, but this was not observed with U5A2-13– TCRα β+ cells. To the best of our knowledge, this is the first direct evidence of the presence of NK-like T cells defined phenotypically by U5A2-13 mAb and functionally by IL-4/IFN-γ production in NK1.1-negative mouse strains. We also demonstrated that U5A2-13– NK1.1+ T cells and U5A2-13+ NK1.1– T cells in C57BL/6 mice could produce both IL-4 and IFN-γ. In addition, Vβ8 or Vβ7 usage by U5A2-13+ NK1.1– T cells was lower than that by U5A2-13+ NK1.1+ T cells, but remained higher than that by U5A2-13– NK1.1– T cells. Based on the present results, U5A2-13 mAb appears to be a valuable tool in the study of NK-like T cells." @default.
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• W2019071427 date "1999-06-01" @default.
• W2019071427 modified "2023-09-25" @default.
• W2019071427 title "Functional and phenotypical characteristics of hepatic NK-like T cells in NK1.1-positive and -negative mouse strains" @default.
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• W2019071427 doi "https://doi.org/10.1002/(sici)1521-4141(199906)29:06<1871::aid-immu1871>3.0.co;2-d" @default.
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