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• W2019075506 abstract "To permit facile 15N solid-state NMR (ssNMR) analysis of the degree of acetylation (DA) of chitinous materials in fungi a method for the introduction of a 15N isotopic label has been developed. Using Penicillium chrysogenum as a model system, a series of 15N-based media were surveyed for their abilities to support mycelial growth, and a rich medium supplemented with (15NH4)2SO4 supported good growth. Uptake of label into chitin extracted from mycelia grown in the rich (15NH4)2SO4-based media was monitored by mass spectrometry, with ∼1 g/L of (15NH4)2SO4 leading to ∼65% incorporation. The labeled chitin was studied by ssNMR to determine its DA, and the 15N label permitted measurement of DA to within 0.5% with acquisition times of on the order of half an hour. Similar studies validated the method for DA measurements on chitin from cultures of Aspergillus niger and Mucor rouxii." @default.
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• W2019075506 date "2009-02-27" @default.
• W2019075506 modified "2023-09-24" @default.
• W2019075506 title "An Efficient Method for ¹⁵N-Labeling of Chitin in Fungi" @default.
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• W2019075506 doi "https://doi.org/10.1021/bm8012814" @default.
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