Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019087218> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2019087218 endingPage "6393" @default.
- W2019087218 startingPage "6386" @default.
- W2019087218 abstract "Sarcoplasmic reticulum Ca2+-ATPase is an ion pump whose catalytic cycle includes the transient formation of an acyl phosphate at Asp351, and fluorescein isothiocyanate is a covalent inhibitor of ATP binding to this pump, known to specifically derivatize Lys515 in the nucleotide-binding site. It was previously found that an unusually stable, phosphorylated form of fluorescein-ATPase, with low fluorescence, is obtained following Ca2+ loading with acetyl phosphate as energy source and then chelation with EGTA of Ca2+ on the cytosolic side. Here we show that the phospho-linkage in this low fluorescent species is stable at alkaline pH, unlike the acyl phosphate at Asp351. Moreover, the low fluorescence and stable phosphoryl group track together in primary and secondary tryptic subfragments, separated by SDS−PAGE after denaturation. Finally, normal fluorescence and absorbance are recovered upon treatment with alkaline phosphatase after extensive trypsinolysis. We conclude that the low fluorescent species is the result of the phosphoryl group being transferred from Asp351 to the fluorescein moiety during pump reversal, yielding fluorescein monophosphate tethered to Ca2+-ATPase." @default.
- W2019087218 created "2016-06-24" @default.
- W2019087218 creator A5001939732 @default.
- W2019087218 creator A5025922191 @default.
- W2019087218 creator A5072718587 @default.
- W2019087218 date "2008-05-24" @default.
- W2019087218 modified "2023-10-13" @default.
- W2019087218 title "Unexpected Phosphoryl Transfer from Asp<sup>351</sup> to Fluorescein Attached to Lys515 in Sarcoplasmic Reticulum Ca<sup>2+</sup>-ATPase" @default.
- W2019087218 cites W1499131945 @default.
- W2019087218 cites W1515311330 @default.
- W2019087218 cites W1586975738 @default.
- W2019087218 cites W1966112781 @default.
- W2019087218 cites W1977822260 @default.
- W2019087218 cites W1988393869 @default.
- W2019087218 cites W1989639565 @default.
- W2019087218 cites W1990708284 @default.
- W2019087218 cites W2009346539 @default.
- W2019087218 cites W2009740509 @default.
- W2019087218 cites W2029313572 @default.
- W2019087218 cites W2050873218 @default.
- W2019087218 cites W2062572341 @default.
- W2019087218 cites W2072830471 @default.
- W2019087218 cites W2076588074 @default.
- W2019087218 cites W2094218961 @default.
- W2019087218 cites W2100837269 @default.
- W2019087218 cites W2109503333 @default.
- W2019087218 cites W2149338396 @default.
- W2019087218 cites W44234124 @default.
- W2019087218 doi "https://doi.org/10.1021/bi800290q" @default.
- W2019087218 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18500824" @default.
- W2019087218 hasPublicationYear "2008" @default.
- W2019087218 type Work @default.
- W2019087218 sameAs 2019087218 @default.
- W2019087218 citedByCount "5" @default.
- W2019087218 countsByYear W20190872182012 @default.
- W2019087218 countsByYear W20190872182014 @default.
- W2019087218 crossrefType "journal-article" @default.
- W2019087218 hasAuthorship W2019087218A5001939732 @default.
- W2019087218 hasAuthorship W2019087218A5025922191 @default.
- W2019087218 hasAuthorship W2019087218A5072718587 @default.
- W2019087218 hasConcept C101571972 @default.
- W2019087218 hasConcept C104317684 @default.
- W2019087218 hasConcept C121332964 @default.
- W2019087218 hasConcept C158617107 @default.
- W2019087218 hasConcept C181199279 @default.
- W2019087218 hasConcept C185592680 @default.
- W2019087218 hasConcept C23265538 @default.
- W2019087218 hasConcept C2776568683 @default.
- W2019087218 hasConcept C2777019933 @default.
- W2019087218 hasConcept C2777132085 @default.
- W2019087218 hasConcept C2778015030 @default.
- W2019087218 hasConcept C512185932 @default.
- W2019087218 hasConcept C55493867 @default.
- W2019087218 hasConcept C62520636 @default.
- W2019087218 hasConcept C71240020 @default.
- W2019087218 hasConcept C91881484 @default.
- W2019087218 hasConceptScore W2019087218C101571972 @default.
- W2019087218 hasConceptScore W2019087218C104317684 @default.
- W2019087218 hasConceptScore W2019087218C121332964 @default.
- W2019087218 hasConceptScore W2019087218C158617107 @default.
- W2019087218 hasConceptScore W2019087218C181199279 @default.
- W2019087218 hasConceptScore W2019087218C185592680 @default.
- W2019087218 hasConceptScore W2019087218C23265538 @default.
- W2019087218 hasConceptScore W2019087218C2776568683 @default.
- W2019087218 hasConceptScore W2019087218C2777019933 @default.
- W2019087218 hasConceptScore W2019087218C2777132085 @default.
- W2019087218 hasConceptScore W2019087218C2778015030 @default.
- W2019087218 hasConceptScore W2019087218C512185932 @default.
- W2019087218 hasConceptScore W2019087218C55493867 @default.
- W2019087218 hasConceptScore W2019087218C62520636 @default.
- W2019087218 hasConceptScore W2019087218C71240020 @default.
- W2019087218 hasConceptScore W2019087218C91881484 @default.
- W2019087218 hasIssue "24" @default.
- W2019087218 hasLocation W20190872181 @default.
- W2019087218 hasLocation W20190872182 @default.
- W2019087218 hasLocation W20190872183 @default.
- W2019087218 hasLocation W20190872184 @default.
- W2019087218 hasOpenAccess W2019087218 @default.
- W2019087218 hasPrimaryLocation W20190872181 @default.
- W2019087218 hasRelatedWork W2032816982 @default.
- W2019087218 hasRelatedWork W2033193375 @default.
- W2019087218 hasRelatedWork W2034796571 @default.
- W2019087218 hasRelatedWork W2050723662 @default.
- W2019087218 hasRelatedWork W2054145795 @default.
- W2019087218 hasRelatedWork W2064174632 @default.
- W2019087218 hasRelatedWork W2092718165 @default.
- W2019087218 hasRelatedWork W2315085743 @default.
- W2019087218 hasRelatedWork W2381772152 @default.
- W2019087218 hasRelatedWork W2479076116 @default.
- W2019087218 hasVolume "47" @default.
- W2019087218 isParatext "false" @default.
- W2019087218 isRetracted "false" @default.
- W2019087218 magId "2019087218" @default.
- W2019087218 workType "article" @default.