FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019091954> ?p ?o ?g. }

• W2019091954 endingPage "518" @default.
• W2019091954 startingPage "509" @default.
• W2019091954 abstract "The prion protein (PrP(C)) is a copper binding cell surface glycoprotein which when misfolded causes transmissible spongiform encephalopathies. The cooperative binding of Cu2+ to an unstructured octarepeat sequence within PrP(C) causes profound folding of this region. The use of NMR to determine the solution structure of the octarepeat region of PrP with Cu2+ bound has been hampered by the paramagnetic nature of the Cu2+ ions. Using NMR we have investigated the binding of candidate diamagnetic replacement ions, to the octarepeat region of PrP. We show that Pd2+ forms diamagnetic complexes with the peptides HGGG, HGGGW and QPHGGGWGQ with 1:1 stoichiometry. The 1H NMR spectra indicate that these peptides are in slow-exchange between free and bound Pd2+ on the chemical-shift time-scale. We demonstrate that the Pd-peptide complex forms slowly with a time taken to reach half-maximal signal of 3 hours. Other candidate metal ions, Ni2+, Pt2+ and Au3+, were investigated but only the Pd2+ complexes gave resolvable 1H NMR spectra. We have determined the solution structure of the QPHGGGWGQ-Pd 1:1 complex using 71 NOE distance restraints. A backbone RMSD of 0.30 A was observed over residues 3 to 7 in the final ensemble. The co-ordinating ligands consist of the histidine imidazole side chain N epsilon, the amide N of the second and third glycines with possibly H2O as the fourth ligand. The co-ordination geometry differs markedly from that of the HGGGW-Cu crystal structure. This survey of potential replacement metal ions to Cu2+ provides insight into the metal specificity and co-ordination chemistry of the metal bound octarepeats." @default.
• W2019091954 created "2016-06-24" @default.
• W2019091954 creator A5008280279 @default.
• W2019091954 creator A5027302343 @default.
• W2019091954 creator A5083779822 @default.
• W2019091954 date "2006-01-01" @default.
• W2019091954 modified "2023-09-25" @default.
• W2019091954 title "A survey of diamagnetic probes for copper²⁺binding to the prion protein.¹H NMR solution structure of the palladium²⁺bound single octarepeat" @default.
• W2019091954 cites W149247114 @default.
• W2019091954 cites W1535032604 @default.
• W2019091954 cites W1575128625 @default.
• W2019091954 cites W1932252362 @default.
• W2019091954 cites W1965483182 @default.
• W2019091954 cites W1974288679 @default.
• W2019091954 cites W1975459052 @default.
• W2019091954 cites W1976624408 @default.
• W2019091954 cites W1976951513 @default.
• W2019091954 cites W1978169903 @default.
• W2019091954 cites W1984676214 @default.
• W2019091954 cites W1995455102 @default.
• W2019091954 cites W2002236213 @default.
• W2019091954 cites W2003129868 @default.
• W2019091954 cites W2005986251 @default.
• W2019091954 cites W2010388034 @default.
• W2019091954 cites W2014235932 @default.
• W2019091954 cites W2017634178 @default.
• W2019091954 cites W2022058058 @default.
• W2019091954 cites W2022735447 @default.
• W2019091954 cites W2023918463 @default.
• W2019091954 cites W2024954984 @default.
• W2019091954 cites W2032545721 @default.
• W2019091954 cites W2032648665 @default.
• W2019091954 cites W2036884196 @default.
• W2019091954 cites W2045261923 @default.
• W2019091954 cites W2057293794 @default.
• W2019091954 cites W2058659733 @default.
• W2019091954 cites W2068602112 @default.
• W2019091954 cites W2070458959 @default.
• W2019091954 cites W2070596466 @default.
• W2019091954 cites W2076146345 @default.
• W2019091954 cites W2076972075 @default.
• W2019091954 cites W2077281287 @default.
• W2019091954 cites W2083050608 @default.
• W2019091954 cites W2087105167 @default.
• W2019091954 cites W2091705326 @default.
• W2019091954 cites W2092063619 @default.
• W2019091954 cites W2093399444 @default.
• W2019091954 cites W2096510701 @default.
• W2019091954 cites W2101783641 @default.
• W2019091954 cites W2122708921 @default.
• W2019091954 cites W2138038687 @default.
• W2019091954 cites W2138768135 @default.
• W2019091954 cites W2141663125 @default.
• W2019091954 cites W2143795630 @default.
• W2019091954 cites W2147276565 @default.
• W2019091954 cites W2155389749 @default.
• W2019091954 cites W2157784883 @default.
• W2019091954 cites W2157980298 @default.
• W2019091954 cites W2159768773 @default.
• W2019091954 cites W2162166182 @default.
• W2019091954 cites W2164960448 @default.
• W2019091954 cites W2167564565 @default.
• W2019091954 cites W2324660492 @default.
• W2019091954 cites W4240171860 @default.
• W2019091954 doi "https://doi.org/10.1039/b511553a" @default.
• W2019091954 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16395451" @default.
• W2019091954 hasPublicationYear "2006" @default.
• W2019091954 type Work @default.
• W2019091954 sameAs 2019091954 @default.
• W2019091954 citedByCount "23" @default.
• W2019091954 countsByYear W20190919542012 @default.
• W2019091954 countsByYear W20190919542013 @default.
• W2019091954 countsByYear W20190919542014 @default.
• W2019091954 countsByYear W20190919542016 @default.
• W2019091954 countsByYear W20190919542022 @default.
• W2019091954 crossrefType "journal-article" @default.
• W2019091954 hasAuthorship W2019091954A5008280279 @default.
• W2019091954 hasAuthorship W2019091954A5027302343 @default.
• W2019091954 hasAuthorship W2019091954A5083779822 @default.
• W2019091954 hasConcept C103319777 @default.
• W2019091954 hasConcept C115260700 @default.
• W2019091954 hasConcept C116569031 @default.
• W2019091954 hasConcept C121332964 @default.
• W2019091954 hasConcept C1276947 @default.
• W2019091954 hasConcept C144082473 @default.
• W2019091954 hasConcept C145148216 @default.
• W2019091954 hasConcept C147789679 @default.
• W2019091954 hasConcept C170493617 @default.
• W2019091954 hasConcept C178790620 @default.
• W2019091954 hasConcept C185592680 @default.
• W2019091954 hasConcept C2778439535 @default.
• W2019091954 hasConcept C2778460671 @default.
• W2019091954 hasConcept C4839761 @default.
• W2019091954 hasConcept C515207424 @default.
• W2019091954 hasConcept C544778455 @default.
• W2019091954 hasConcept C55493867 @default.
• W2019091954 hasConcept C62520636 @default.
• W2019091954 hasConcept C66974803 @default.

• next