Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019100799> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2019100799 endingPage "52" @default.
- W2019100799 startingPage "40" @default.
- W2019100799 abstract "Substrate-limited fed-batch cultures were used to study growth and overflow metabolism in hybridoma and insect cells. In hybridoma cells a glucose-limited fed-batch culture decreased lactate formation but increased glutamine consumption and ammonium formation. Glutamine limitation decreased ammonium and alanine formation but did not enhance glucose consumption. Instead lactate formation was reduced, indicating that glucose was used more efficiently. The formation of lactate, alanine, and ammonium was negligible in a dual substrate-limited fed-batch culture. The efficiency of the energy metabolism increased, as judged by the increase in the cellular yield coefficient for glucose of 100% and for glutamine of 150% and by the change in the metabolic ratios lac/glc, ala/gln, and NHx/gln, in the combined fed-batch culture. Insect cell metabolism was studied in Spodoptera frugiperda (Sf-9) cells. A stringent relation between glucose excess and alanine formation was found. In contrast, glucose limitation induced ammonium formation, while, at the same time, alanine formation was completely suppressed. Simultaneous glucose and glutamine limitation suppressed both alanine and ammonium formation. Alanine formation appears as wasteful as lactate formation because the growth rate of insect cells in substrate-limited cultures was the same as in batch cultures with substrate excess. In batch and fed-batch cultures of both cell lines, mu reaches it maximum early during growth and decreases thereafter so that no exponential growth occurs. The growth rate limiting factor for hybridoma cells was found to be a component of serum, because intermittent serum additions to batch cultures resulted in a high and constant growth rate. Insulin was identified as the main cause, inasmuch as intermittent insulin additions gave the same result as serum." @default.
- W2019100799 created "2016-06-24" @default.
- W2019100799 creator A5002953134 @default.
- W2019100799 creator A5024467012 @default.
- W2019100799 creator A5039504931 @default.
- W2019100799 date "1996-05-01" @default.
- W2019100799 modified "2023-10-03" @default.
- W2019100799 title "Metabolic Engineering of Animal Cellsa" @default.
- W2019100799 cites W1483212110 @default.
- W2019100799 cites W169847390 @default.
- W2019100799 cites W190028184 @default.
- W2019100799 cites W1967647423 @default.
- W2019100799 cites W1970639534 @default.
- W2019100799 cites W1986919632 @default.
- W2019100799 cites W1996380733 @default.
- W2019100799 cites W2018727351 @default.
- W2019100799 cites W2025909787 @default.
- W2019100799 cites W2041746790 @default.
- W2019100799 cites W2076311012 @default.
- W2019100799 cites W2141172423 @default.
- W2019100799 cites W2167512342 @default.
- W2019100799 doi "https://doi.org/10.1111/j.1749-6632.1996.tb40545.x" @default.
- W2019100799 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8659912" @default.
- W2019100799 hasPublicationYear "1996" @default.
- W2019100799 type Work @default.
- W2019100799 sameAs 2019100799 @default.
- W2019100799 citedByCount "18" @default.
- W2019100799 countsByYear W20191007992012 @default.
- W2019100799 countsByYear W20191007992013 @default.
- W2019100799 countsByYear W20191007992018 @default.
- W2019100799 crossrefType "journal-article" @default.
- W2019100799 hasAuthorship W2019100799A5002953134 @default.
- W2019100799 hasAuthorship W2019100799A5024467012 @default.
- W2019100799 hasAuthorship W2019100799A5039504931 @default.
- W2019100799 hasConcept C100544194 @default.
- W2019100799 hasConcept C167431671 @default.
- W2019100799 hasConcept C17093226 @default.
- W2019100799 hasConcept C178790620 @default.
- W2019100799 hasConcept C185592680 @default.
- W2019100799 hasConcept C18903297 @default.
- W2019100799 hasConcept C2777289219 @default.
- W2019100799 hasConcept C2779349466 @default.
- W2019100799 hasConcept C2779856020 @default.
- W2019100799 hasConcept C2779870022 @default.
- W2019100799 hasConcept C515207424 @default.
- W2019100799 hasConcept C55493867 @default.
- W2019100799 hasConcept C62231903 @default.
- W2019100799 hasConcept C86803240 @default.
- W2019100799 hasConceptScore W2019100799C100544194 @default.
- W2019100799 hasConceptScore W2019100799C167431671 @default.
- W2019100799 hasConceptScore W2019100799C17093226 @default.
- W2019100799 hasConceptScore W2019100799C178790620 @default.
- W2019100799 hasConceptScore W2019100799C185592680 @default.
- W2019100799 hasConceptScore W2019100799C18903297 @default.
- W2019100799 hasConceptScore W2019100799C2777289219 @default.
- W2019100799 hasConceptScore W2019100799C2779349466 @default.
- W2019100799 hasConceptScore W2019100799C2779856020 @default.
- W2019100799 hasConceptScore W2019100799C2779870022 @default.
- W2019100799 hasConceptScore W2019100799C515207424 @default.
- W2019100799 hasConceptScore W2019100799C55493867 @default.
- W2019100799 hasConceptScore W2019100799C62231903 @default.
- W2019100799 hasConceptScore W2019100799C86803240 @default.
- W2019100799 hasIssue "1" @default.
- W2019100799 hasLocation W20191007991 @default.
- W2019100799 hasLocation W20191007992 @default.
- W2019100799 hasOpenAccess W2019100799 @default.
- W2019100799 hasPrimaryLocation W20191007991 @default.
- W2019100799 hasRelatedWork W1537421998 @default.
- W2019100799 hasRelatedWork W1963548841 @default.
- W2019100799 hasRelatedWork W2019100799 @default.
- W2019100799 hasRelatedWork W2041746790 @default.
- W2019100799 hasRelatedWork W205817276 @default.
- W2019100799 hasRelatedWork W2088389091 @default.
- W2019100799 hasRelatedWork W2149750411 @default.
- W2019100799 hasRelatedWork W2439413971 @default.
- W2019100799 hasRelatedWork W607604077 @default.
- W2019100799 hasRelatedWork W1966800994 @default.
- W2019100799 hasVolume "782" @default.
- W2019100799 isParatext "false" @default.
- W2019100799 isRetracted "false" @default.
- W2019100799 magId "2019100799" @default.
- W2019100799 workType "article" @default.