FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019113609> ?p ?o ?g. }

• W2019113609 endingPage "e77398" @default.
• W2019113609 startingPage "e77398" @default.
• W2019113609 abstract "Background Therapeutic antibody development is one of the fastest growing areas of the pharmaceutical industry. Generating high-quality monoclonal antibodies against a given therapeutic target is crucial for successful drug development. However, due to immune tolerance, making it difficult to generate antibodies using conventional approaches. Methodology/Findings Mixed four human gastric cancer (GC) cell lines were used as the immunogen in A/J mice; sixteen highly positive hybridoma colonies were selected via fluorescence-activated cell sorting-high throughput screening (FACS-HTS) using a total of 20,000 colonies in sixty-seven 96-well plates against live cells (mixed human GC cells versus human PBMC controls). MS17-57 and control commercial Alkaline Phosphatase (ALP) mAbs were used to confirm the target antigens (Ags), which were identified as ALPs expressed on the GC cell surface through a combination of western blot, immunoprecipitation and mass spectrometry (MS). MS identified the Ags recognized by MS17-57 to be two variants of a secreted ALP, PALP and IALP (Placental and intestinal ALP). These proteins belong to a hydrolase enzyme family responsible for removing phosphate groups from many types of molecules. Immunofluorescence staining using MS17-57 demonstrated higher staining of gastrointestinal (GI) cancer tissues compared to normal GI tissues (P<0.03), and confirmed binding of MS17-57 to be restricted to a functional epitope expressed on the cancer cell surface. Proliferation assays using the PALP/IALP-expressing GC cell lines demonstrated that MS17-57 inhibited cell growth by 32±8%. Transwell cell migration assays documented that MS17-57 can inhibit PALP/IALP-expressing GI cancer cell migration by 25±5%. MS17-57 mAb inhibited tumor growth in nude mice. Conclusions Our findings indicate that PALP and IALP can be ectopically expressed on extracellular matrix of GI cancers, and that MS17-57 directed against PALP/IALP can inhibit GI cancer cells growth and migration in vitro and in vivo. This investigation provides an example of identification of cancer biomarkers representing promising therapeutic targets using mAb generated through a novel HTS technology." @default.
• W2019113609 created "2016-06-24" @default.
• W2019113609 creator A5016908661 @default.
• W2019113609 creator A5020005002 @default.
• W2019113609 creator A5022491856 @default.
• W2019113609 creator A5042341684 @default.
• W2019113609 creator A5043366079 @default.
• W2019113609 creator A5051133445 @default.
• W2019113609 creator A5060456533 @default.
• W2019113609 creator A5062046621 @default.
• W2019113609 creator A5063310226 @default.
• W2019113609 creator A5068065230 @default.
• W2019113609 creator A5080625732 @default.
• W2019113609 creator A5084178846 @default.
• W2019113609 creator A5088523673 @default.
• W2019113609 date "2013-10-15" @default.
• W2019113609 modified "2023-09-26" @default.
• W2019113609 title "Generation of Monoclonal Antibody MS17-57 Targeting Secreted Alkaline Phosphatase Ectopically Expressed on the Surface of Gastrointestinal Cancer Cells" @default.
• W2019113609 cites W1119605895 @default.
• W2019113609 cites W1538077234 @default.
• W2019113609 cites W1571122109 @default.
• W2019113609 cites W1870003501 @default.
• W2019113609 cites W1973875958 @default.
• W2019113609 cites W1981203968 @default.
• W2019113609 cites W1995642474 @default.
• W2019113609 cites W2014079612 @default.
• W2019113609 cites W2021974025 @default.
• W2019113609 cites W2028422263 @default.
• W2019113609 cites W2030522563 @default.
• W2019113609 cites W2044875778 @default.
• W2019113609 cites W2049432501 @default.
• W2019113609 cites W2050247332 @default.
• W2019113609 cites W2053245222 @default.
• W2019113609 cites W2066671159 @default.
• W2019113609 cites W2083445704 @default.
• W2019113609 cites W2088321060 @default.
• W2019113609 cites W2089652099 @default.
• W2019113609 cites W2096806100 @default.
• W2019113609 cites W2098688481 @default.
• W2019113609 cites W2100602909 @default.
• W2019113609 cites W2107816553 @default.
• W2019113609 cites W2111819232 @default.
• W2019113609 cites W2151417485 @default.
• W2019113609 cites W2157925286 @default.
• W2019113609 cites W2169434055 @default.
• W2019113609 cites W4317676558 @default.
• W2019113609 doi "https://doi.org/10.1371/journal.pone.0077398" @default.
• W2019113609 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3797039" @default.
• W2019113609 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24143229" @default.
• W2019113609 hasPublicationYear "2013" @default.
• W2019113609 type Work @default.
• W2019113609 sameAs 2019113609 @default.
• W2019113609 citedByCount "5" @default.
• W2019113609 countsByYear W20191136092014 @default.
• W2019113609 countsByYear W20191136092015 @default.
• W2019113609 countsByYear W20191136092018 @default.
• W2019113609 countsByYear W20191136092020 @default.
• W2019113609 crossrefType "journal-article" @default.
• W2019113609 hasAuthorship W2019113609A5016908661 @default.
• W2019113609 hasAuthorship W2019113609A5020005002 @default.
• W2019113609 hasAuthorship W2019113609A5022491856 @default.
• W2019113609 hasAuthorship W2019113609A5042341684 @default.
• W2019113609 hasAuthorship W2019113609A5043366079 @default.
• W2019113609 hasAuthorship W2019113609A5051133445 @default.
• W2019113609 hasAuthorship W2019113609A5060456533 @default.
• W2019113609 hasAuthorship W2019113609A5062046621 @default.
• W2019113609 hasAuthorship W2019113609A5063310226 @default.
• W2019113609 hasAuthorship W2019113609A5068065230 @default.
• W2019113609 hasAuthorship W2019113609A5080625732 @default.
• W2019113609 hasAuthorship W2019113609A5084178846 @default.
• W2019113609 hasAuthorship W2019113609A5088523673 @default.
• W2019113609 hasBestOaLocation W20191136091 @default.
• W2019113609 hasConcept C147483822 @default.
• W2019113609 hasConcept C153911025 @default.
• W2019113609 hasConcept C159654299 @default.
• W2019113609 hasConcept C160160445 @default.
• W2019113609 hasConcept C181199279 @default.
• W2019113609 hasConcept C195616568 @default.
• W2019113609 hasConcept C203014093 @default.
• W2019113609 hasConcept C2778236600 @default.
• W2019113609 hasConcept C2778630268 @default.
• W2019113609 hasConcept C2779909307 @default.
• W2019113609 hasConcept C2780025047 @default.
• W2019113609 hasConcept C542903549 @default.
• W2019113609 hasConcept C54355233 @default.
• W2019113609 hasConcept C553184892 @default.
• W2019113609 hasConcept C55493867 @default.
• W2019113609 hasConcept C62112901 @default.
• W2019113609 hasConcept C81885089 @default.
• W2019113609 hasConcept C86803240 @default.
• W2019113609 hasConceptScore W2019113609C147483822 @default.
• W2019113609 hasConceptScore W2019113609C153911025 @default.
• W2019113609 hasConceptScore W2019113609C159654299 @default.
• W2019113609 hasConceptScore W2019113609C160160445 @default.
• W2019113609 hasConceptScore W2019113609C181199279 @default.
• W2019113609 hasConceptScore W2019113609C195616568 @default.
• W2019113609 hasConceptScore W2019113609C203014093 @default.
• W2019113609 hasConceptScore W2019113609C2778236600 @default.

• next