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- W2019115613 abstract "Single/low-copy transgene integration is essential for avoiding overexpression, ectopic expression and gene silencing in the germline. Here, we present an overview of a method that uses ultraviolet and trimethylpsoralen (UV/TMP) to generate single/low-copy gene integrations in Caenorhabditis elegans. Single/low-copy transgenes from extrachromosomal arrays are integrated into the genome using positive selection based on temperature sensitivity with a vps-45 rescue fragment and negative selection based on benzimidazole sensitivity with a ben-1 rescue fragment. The copy number of the integrated transgenes is determined using quantitative PCR. Our UV/TMP integration method, which is based on familiar extrachromosomal transgenics, provides a simple approach for generating single/low-copy gene integrations." @default.
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- W2019115613 date "2014-08-01" @default.
- W2019115613 modified "2023-09-25" @default.
- W2019115613 title "Methods for single/low-copy integration by ultraviolet and trimethylpsoralen treatment in Caenorhabditis elegans" @default.
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- W2019115613 doi "https://doi.org/10.1016/j.ymeth.2014.02.036" @default.
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