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- W2019130993 abstract "1. The production of a H+ gradient across the isolated frog skin was qualitatively and quantitatively studied in pieces of ventral skin mounted in Ussing-type chambers and maintained in CO2-free conditions. 2. Acidification of the outer bathing medium and alkalinization of the inner bathing medium were evaluated either by pH measurement or by the pH-stat technique. 3. An asymmetry of the two phenomena was manifested by a different behaviour in time and by a greater amount of base added to the inner chamber as compared with the amount of acid added to the outer chamber. 4. Acidification was determined by the extrusion of H+ generated intracellularly. 5. The dependence of acidification on the presence of O2 and its suppression by a carbonic anhydrase inhibitor suggest that the H+ are provided by the intracellular hydration of metabolic CO2. 6. A fraction of the added base could be stoichiometrically related to the acid added." @default.
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- W2019130993 title "The production of a hydrogen ion gradient across the isolated frog skin quantitative aspects and the effect of acetazolamide" @default.
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- W2019130993 doi "https://doi.org/10.1016/0005-2736(70)90180-x" @default.
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