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• W2019144003 abstract "To determine the presence of enterohaemorrhagic Escherichia coli (EHEC), 50 water samples of three different lakes in Dhaka, Bangladesh were studied. A total of 90 strains were examined thoroughly to isolate sorbitol non-fermenting (NSF) E. coli. Twelve of 90 isolates (13.3%) from 5 samples were found to be sorbitol nonfermenter, which were further screened for rhamnose, cellobiose and sucrose fermentation. Eleven (12.2%) of 90 isolates also tested negative for cellobiose and sucrose fermentation, while only 3 (3.3%) of them were found to be rhamnose non-fermenting. Biochemical tests confirmed that 91.7% (11 of 12) of NSF strains were indole positive. Eleven NSF E. coli isolates survived at 45.5°C and mostly produced gas by fermentation within 24h. After antimicrobial susceptibility testing, 91.7% (n=11) of these isolates were found resistant to cephalexin, ampicillin and amoxicillin. Ceftazidime and nalidixic acid resistance was also observed in 75% (9 of 12) and 58.3% (7 of 12) isolates, respectively. Plasmid profile analysis revealed that nine (75%) strains harboured large plasmids of >35.8-140 megadaltons (MDa), four (33.3%) of which contained 60-MDa plasmid. Also, the large-plasmid-associated strains shared common behaviour in antimicrobial resistance and eight (88.9%) of these were resistant to four or more antibiotics. Our data suggest a possible association of source water contamination in Bangladesh with multidrug-resistant (MDR) EHEC, which is alarming to public health. DOI: http://dx.doi.org/10.3329/bjm.v28i1.11807 Bangladesh J Microbiol, Volume 28, Number 1, June 2011, pp 33-40" @default.
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• W2019144003 date "2012-09-04" @default.
• W2019144003 modified "2023-10-07" @default.
• W2019144003 title "Multidrug Resistance in Large-Plasmid-Associated Presumptive Enterohaemorrhagic Escherichia coli Isolated from Contaminated Lake Water" @default.
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• W2019144003 doi "https://doi.org/10.3329/bjm.v28i1.11807" @default.
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