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- W2019175816 abstract "INTRODUCTION The choice of fixation and permeabilization methods for examining flies depends on their stage of development. Early embryos (0-17 hours or until cuticle formation) are treated with a mixture of organic solvents, formaldehyde, and alcohols, as described here. The cuticles of late-stage embryos are usually opened by sonication. Tissues from more advanced stages of development are normally dissected by hand and then fixed and stained in a standard paraformaldehyde/detergent combination" @default.
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- W2019175816 date "2006-09-01" @default.
- W2019175816 modified "2023-10-16" @default.
- W2019175816 title "Preparing Early Whole-Mount <i>Drosophila</i> Embryos for Immunostaining" @default.
- W2019175816 doi "https://doi.org/10.1101/pdb.prot4524" @default.
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