Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019202272> ?p ?o ?g. }
- W2019202272 endingPage "779" @default.
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- W2019202272 abstract "Abstract Self-incompatibility (SI) is an important genetically controlled mechanism to prevent inbreeding in higher plants. SI involves highly specific interactions during pollination, resulting in the rejection of incompatible (self) pollen. Programmed cell death (PCD) is an important mechanism for destroying cells in a precisely regulated manner. SI in field poppy (Papaver rhoeas) triggers PCD in incompatible pollen. During SI-induced PCD, we previously observed a major acidification of the pollen cytosol. Here, we present measurements of temporal alterations in cytosolic pH ([pH]cyt); they were surprisingly rapid, reaching pH 6.4 within 10 min of SI induction and stabilizing by 60 min at pH 5.5. By manipulating the [pH]cyt of the pollen tubes in vivo, we show that [pH]cyt acidification is an integral and essential event for SI-induced PCD. Here, we provide evidence showing the physiological relevance of the cytosolic acidification and identify key targets of this major physiological alteration. A small drop in [pH]cyt inhibits the activity of a soluble inorganic pyrophosphatase required for pollen tube growth. We also show that [pH]cyt acidification is necessary and sufficient for triggering several key hallmark features of the SI PCD signaling pathway, notably activation of a DEVDase/caspase-3-like activity and formation of SI-induced punctate actin foci. Importantly, the actin binding proteins Cyclase-Associated Protein and Actin-Depolymerizing Factor are identified as key downstream targets. Thus, we have shown the biological relevance of an extreme but physiologically relevant alteration in [pH]cyt and its effect on several components in the context of SI-induced events and PCD." @default.
- W2019202272 created "2016-06-24" @default.
- W2019202272 creator A5022766057 @default.
- W2019202272 creator A5045002362 @default.
- W2019202272 creator A5048089032 @default.
- W2019202272 creator A5055712563 @default.
- W2019202272 creator A5080234864 @default.
- W2019202272 creator A5085150089 @default.
- W2019202272 date "2015-01-28" @default.
- W2019202272 modified "2023-10-01" @default.
- W2019202272 title "Self-Incompatibility-Induced Programmed Cell Death in Field Poppy Pollen Involves Dramatic Acidification of the Incompatible Pollen Tube Cytosol " @default.
- W2019202272 cites W1520440482 @default.
- W2019202272 cites W1536708917 @default.
- W2019202272 cites W1943234654 @default.
- W2019202272 cites W1968289383 @default.
- W2019202272 cites W1969950216 @default.
- W2019202272 cites W1978489676 @default.
- W2019202272 cites W1981705025 @default.
- W2019202272 cites W1982637041 @default.
- W2019202272 cites W1982719431 @default.
- W2019202272 cites W1987149333 @default.
- W2019202272 cites W1999350913 @default.
- W2019202272 cites W1999620918 @default.
- W2019202272 cites W2003529245 @default.
- W2019202272 cites W2005330836 @default.
- W2019202272 cites W2005431883 @default.
- W2019202272 cites W2007517675 @default.
- W2019202272 cites W2010209292 @default.
- W2019202272 cites W2011984248 @default.
- W2019202272 cites W2017184363 @default.
- W2019202272 cites W2018178015 @default.
- W2019202272 cites W2024077782 @default.
- W2019202272 cites W2025922718 @default.
- W2019202272 cites W2031429450 @default.
- W2019202272 cites W2031726991 @default.
- W2019202272 cites W2042198408 @default.
- W2019202272 cites W2042946762 @default.
- W2019202272 cites W2046591676 @default.
- W2019202272 cites W2046593879 @default.
- W2019202272 cites W2046967457 @default.
- W2019202272 cites W2047550231 @default.
- W2019202272 cites W2047704490 @default.
- W2019202272 cites W2050439032 @default.
- W2019202272 cites W2052245755 @default.
- W2019202272 cites W2062702025 @default.
- W2019202272 cites W2065184846 @default.
- W2019202272 cites W2065954413 @default.
- W2019202272 cites W2068412323 @default.
- W2019202272 cites W2070141177 @default.
- W2019202272 cites W2071708723 @default.
- W2019202272 cites W2074611001 @default.
- W2019202272 cites W2078023563 @default.
- W2019202272 cites W2084804915 @default.
- W2019202272 cites W2085595742 @default.
- W2019202272 cites W2085964673 @default.
- W2019202272 cites W2087413367 @default.
- W2019202272 cites W2089364718 @default.
- W2019202272 cites W2092099782 @default.
- W2019202272 cites W2093863629 @default.
- W2019202272 cites W2094629947 @default.
- W2019202272 cites W2097282003 @default.
- W2019202272 cites W2097559849 @default.
- W2019202272 cites W2099322048 @default.
- W2019202272 cites W2099547318 @default.
- W2019202272 cites W2100194182 @default.
- W2019202272 cites W2100906365 @default.
- W2019202272 cites W2101418944 @default.
- W2019202272 cites W2108073176 @default.
- W2019202272 cites W2110424522 @default.
- W2019202272 cites W2110476295 @default.
- W2019202272 cites W2112060011 @default.
- W2019202272 cites W2113395636 @default.
- W2019202272 cites W2114140792 @default.
- W2019202272 cites W2117093778 @default.
- W2019202272 cites W2119278722 @default.
- W2019202272 cites W2120587073 @default.
- W2019202272 cites W2122228351 @default.
- W2019202272 cites W2122814701 @default.
- W2019202272 cites W2127888098 @default.
- W2019202272 cites W2128262451 @default.
- W2019202272 cites W2128586693 @default.
- W2019202272 cites W2132356542 @default.
- W2019202272 cites W2136790795 @default.
- W2019202272 cites W2137151787 @default.
- W2019202272 cites W2139282609 @default.
- W2019202272 cites W2140005747 @default.
- W2019202272 cites W2141301074 @default.
- W2019202272 cites W2142027485 @default.
- W2019202272 cites W2142943863 @default.
- W2019202272 cites W2143634951 @default.
- W2019202272 cites W2143985087 @default.
- W2019202272 cites W2144679520 @default.
- W2019202272 cites W2147144906 @default.
- W2019202272 cites W2150737511 @default.
- W2019202272 cites W2156001487 @default.
- W2019202272 cites W2156919989 @default.
- W2019202272 cites W2157990737 @default.
- W2019202272 cites W2158164433 @default.