Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019218129> ?p ?o ?g. }
- W2019218129 endingPage "1875" @default.
- W2019218129 startingPage "1864" @default.
- W2019218129 abstract "Cationic antimicrobial peptides (CAMPs) are novel candidates for drug development. Here we describe design of six short and potent CAMPs (SA-1 to SA-6) based on a minimalist template of 12 residues H+HHG+HH+HH+NH2 (where H: hydrophobic amino acid and +: charged hydrophilic amino acid). Designed peptides exhibit good antibacterial activity in micro molar concentration range (1-32 μg/ml) and rapid clearance of Gram-positive and Gram-negative bacterial strains at concentrations higher than MIC. For elucidating mode of action of designed peptides various biophysical studies including CD and Trp fluorescence were performed using model membranes. Further based on activity, selectivity and membrane bound structure; modes of action of Trp rich peptide SA-3 and template based peptide SA-4 were compared. Calcein dye leakage and transmission electron microscopic studies with model membranes exhibited selective membrane active mode of action for peptide SA-3 and SA-4. Extending our work from model membranes to intact E. coli ATCC 11775 in scanning electron micrographs we could visualize different patterns of surface perturbation caused by peptide SA-3 and SA-4. Further at low concentration rapid translocation of FITC-tagged peptide SA-3 into the cytoplasm of E. coli cells without concomitant membrane perturbation indicates involvement of intracellular targeting mechanism as an alternate mode of action as was also evidenced in DNA retardation assay. For peptide SA-4 concentration dependent translocation into the bacterial cytoplasm along with membrane perturbation was observed. Establishment of a non specific membrane lytic mode of action of these peptides makes them suitable candidates for drug development." @default.
- W2019218129 created "2016-06-24" @default.
- W2019218129 creator A5002616102 @default.
- W2019218129 creator A5008940368 @default.
- W2019218129 creator A5017194312 @default.
- W2019218129 creator A5022524918 @default.
- W2019218129 creator A5032122835 @default.
- W2019218129 creator A5056260117 @default.
- W2019218129 creator A5078681138 @default.
- W2019218129 date "2010-10-01" @default.
- W2019218129 modified "2023-09-24" @default.
- W2019218129 title "Interaction studies of novel cell selective antimicrobial peptides with model membranes and E. coli ATCC 11775" @default.
- W2019218129 cites W1513897103 @default.
- W2019218129 cites W1516030996 @default.
- W2019218129 cites W1964546003 @default.
- W2019218129 cites W1964937304 @default.
- W2019218129 cites W1966224201 @default.
- W2019218129 cites W1971756197 @default.
- W2019218129 cites W1975111328 @default.
- W2019218129 cites W1982095205 @default.
- W2019218129 cites W1987113119 @default.
- W2019218129 cites W1989998611 @default.
- W2019218129 cites W1998425508 @default.
- W2019218129 cites W1998601156 @default.
- W2019218129 cites W2003897459 @default.
- W2019218129 cites W2006371387 @default.
- W2019218129 cites W2007561959 @default.
- W2019218129 cites W2007691194 @default.
- W2019218129 cites W2010135598 @default.
- W2019218129 cites W2020799333 @default.
- W2019218129 cites W2020922094 @default.
- W2019218129 cites W2021122013 @default.
- W2019218129 cites W2035769940 @default.
- W2019218129 cites W2036208478 @default.
- W2019218129 cites W2038948629 @default.
- W2019218129 cites W2048779945 @default.
- W2019218129 cites W2049194777 @default.
- W2019218129 cites W2054602599 @default.
- W2019218129 cites W2054983719 @default.
- W2019218129 cites W2056135942 @default.
- W2019218129 cites W2062228103 @default.
- W2019218129 cites W2068863414 @default.
- W2019218129 cites W2075014095 @default.
- W2019218129 cites W2075663982 @default.
- W2019218129 cites W2085136523 @default.
- W2019218129 cites W2087125825 @default.
- W2019218129 cites W2091622692 @default.
- W2019218129 cites W2098978205 @default.
- W2019218129 cites W2110991941 @default.
- W2019218129 cites W2123825516 @default.
- W2019218129 cites W2125702286 @default.
- W2019218129 cites W2130885148 @default.
- W2019218129 cites W2135430766 @default.
- W2019218129 cites W2155482026 @default.
- W2019218129 cites W2167275970 @default.
- W2019218129 cites W2170912840 @default.
- W2019218129 cites W4244732422 @default.
- W2019218129 cites W4245920351 @default.
- W2019218129 doi "https://doi.org/10.1016/j.bbamem.2010.06.016" @default.
- W2019218129 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20599694" @default.
- W2019218129 hasPublicationYear "2010" @default.
- W2019218129 type Work @default.
- W2019218129 sameAs 2019218129 @default.
- W2019218129 citedByCount "72" @default.
- W2019218129 countsByYear W20192181292012 @default.
- W2019218129 countsByYear W20192181292013 @default.
- W2019218129 countsByYear W20192181292014 @default.
- W2019218129 countsByYear W20192181292015 @default.
- W2019218129 countsByYear W20192181292016 @default.
- W2019218129 countsByYear W20192181292017 @default.
- W2019218129 countsByYear W20192181292018 @default.
- W2019218129 countsByYear W20192181292019 @default.
- W2019218129 countsByYear W20192181292020 @default.
- W2019218129 countsByYear W20192181292021 @default.
- W2019218129 countsByYear W20192181292022 @default.
- W2019218129 countsByYear W20192181292023 @default.
- W2019218129 crossrefType "journal-article" @default.
- W2019218129 hasAuthorship W2019218129A5002616102 @default.
- W2019218129 hasAuthorship W2019218129A5008940368 @default.
- W2019218129 hasAuthorship W2019218129A5017194312 @default.
- W2019218129 hasAuthorship W2019218129A5022524918 @default.
- W2019218129 hasAuthorship W2019218129A5032122835 @default.
- W2019218129 hasAuthorship W2019218129A5056260117 @default.
- W2019218129 hasAuthorship W2019218129A5078681138 @default.
- W2019218129 hasConcept C121587506 @default.
- W2019218129 hasConcept C12554922 @default.
- W2019218129 hasConcept C185592680 @default.
- W2019218129 hasConcept C190062978 @default.
- W2019218129 hasConcept C2776872141 @default.
- W2019218129 hasConcept C2778106830 @default.
- W2019218129 hasConcept C2779281246 @default.
- W2019218129 hasConcept C41625074 @default.
- W2019218129 hasConcept C540938839 @default.
- W2019218129 hasConcept C55493867 @default.
- W2019218129 hasConcept C86803240 @default.
- W2019218129 hasConceptScore W2019218129C121587506 @default.
- W2019218129 hasConceptScore W2019218129C12554922 @default.
- W2019218129 hasConceptScore W2019218129C185592680 @default.