FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019228926> ?p ?o ?g. }

• W2019228926 endingPage "12250" @default.
• W2019228926 startingPage "12245" @default.
• W2019228926 abstract "We expressed the 52-kDa integral membrane domain (B3mem) of the human erythrocyte anion transporter (band 3; AE1) in a protease-deficient strain of the yeast Saccharomyces cerevisiae under the control of the inducible GAL10-CYC1 promoter. Immunoblots of total protein from transformed yeast cells confirmed that the B3mem polypeptide was overexpressed shortly after induction with galactose. Cell surface expression of the functional anion transporter was detected by using a simple transport assay to measure stilbene disulfonate-inhibitable chloride influx into intact yeast cells. The B3mem polypeptide was recycled and degraded by the cells with a half-life of approximately 1-3 hr, which led to a steady-state level of expression in exponentially growing cultures. Our data suggest that 5-10% of total B3mem is functionally active at the cell surface at any one time and that overexpression of this anion transport protein does not interfere with cell growth or survival. This is one of only a few reports of the functional expression of a plasma membrane transport protein in the plasma membrane of yeast cells and to our knowledge is the first report of red cell band 3-mediated anion transport at the plasma membrane of cDNA-transformed cells. The cell surface expression system we describe will provide a simple means for future study of the functional properties of band 3 by using site-directed mutagenesis." @default.
• W2019228926 created "2016-06-24" @default.
• W2019228926 creator A5033221430 @default.
• W2019228926 creator A5071030097 @default.
• W2019228926 creator A5072229765 @default.
• W2019228926 creator A5089516286 @default.
• W2019228926 date "1996-10-29" @default.
• W2019228926 modified "2023-09-25" @default.
• W2019228926 title "Functional cell surface expression of the anion transport domain of human red cell band 3 (AE1) in the yeast Saccharomyces cerevisiae." @default.
• W2019228926 cites W1484236229 @default.
• W2019228926 cites W1497947649 @default.
• W2019228926 cites W1505533245 @default.
• W2019228926 cites W1506295602 @default.
• W2019228926 cites W1521213011 @default.
• W2019228926 cites W1548346922 @default.
• W2019228926 cites W1559922603 @default.
• W2019228926 cites W1591965351 @default.
• W2019228926 cites W1822034226 @default.
• W2019228926 cites W1970861588 @default.
• W2019228926 cites W2018289835 @default.
• W2019228926 cites W2038344472 @default.
• W2019228926 cites W2051603947 @default.
• W2019228926 cites W2052230164 @default.
• W2019228926 cites W2059237401 @default.
• W2019228926 cites W2062716068 @default.
• W2019228926 cites W2063782395 @default.
• W2019228926 cites W2064119442 @default.
• W2019228926 cites W2064564550 @default.
• W2019228926 cites W2092846883 @default.
• W2019228926 cites W2099602045 @default.
• W2019228926 cites W2100837269 @default.
• W2019228926 cites W2101241746 @default.
• W2019228926 cites W2172683288 @default.
• W2019228926 cites W2181153785 @default.
• W2019228926 cites W222761498 @default.
• W2019228926 cites W2289833142 @default.
• W2019228926 cites W259081840 @default.
• W2019228926 cites W2991971711 @default.
• W2019228926 cites W3176656610 @default.
• W2019228926 cites W4240065971 @default.
• W2019228926 doi "https://doi.org/10.1073/pnas.93.22.12245" @default.
• W2019228926 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/37975" @default.
• W2019228926 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8901565" @default.
• W2019228926 hasPublicationYear "1996" @default.
• W2019228926 type Work @default.
• W2019228926 sameAs 2019228926 @default.
• W2019228926 citedByCount "34" @default.
• W2019228926 countsByYear W20192289262012 @default.
• W2019228926 countsByYear W20192289262013 @default.
• W2019228926 countsByYear W20192289262014 @default.
• W2019228926 countsByYear W20192289262017 @default.
• W2019228926 countsByYear W20192289262020 @default.
• W2019228926 countsByYear W20192289262021 @default.
• W2019228926 countsByYear W20192289262022 @default.
• W2019228926 countsByYear W20192289262023 @default.
• W2019228926 crossrefType "journal-article" @default.
• W2019228926 hasAuthorship W2019228926A5033221430 @default.
• W2019228926 hasAuthorship W2019228926A5071030097 @default.
• W2019228926 hasAuthorship W2019228926A5072229765 @default.
• W2019228926 hasAuthorship W2019228926A5089516286 @default.
• W2019228926 hasBestOaLocation W20192289261 @default.
• W2019228926 hasConcept C115335371 @default.
• W2019228926 hasConcept C144647389 @default.
• W2019228926 hasConcept C1491633281 @default.
• W2019228926 hasConcept C153911025 @default.
• W2019228926 hasConcept C2776589458 @default.
• W2019228926 hasConcept C2777576037 @default.
• W2019228926 hasConcept C2778106830 @default.
• W2019228926 hasConcept C2779222958 @default.
• W2019228926 hasConcept C41625074 @default.
• W2019228926 hasConcept C55493867 @default.
• W2019228926 hasConcept C57581600 @default.
• W2019228926 hasConcept C86803240 @default.
• W2019228926 hasConceptScore W2019228926C115335371 @default.
• W2019228926 hasConceptScore W2019228926C144647389 @default.
• W2019228926 hasConceptScore W2019228926C1491633281 @default.
• W2019228926 hasConceptScore W2019228926C153911025 @default.
• W2019228926 hasConceptScore W2019228926C2776589458 @default.
• W2019228926 hasConceptScore W2019228926C2777576037 @default.
• W2019228926 hasConceptScore W2019228926C2778106830 @default.
• W2019228926 hasConceptScore W2019228926C2779222958 @default.
• W2019228926 hasConceptScore W2019228926C41625074 @default.
• W2019228926 hasConceptScore W2019228926C55493867 @default.
• W2019228926 hasConceptScore W2019228926C57581600 @default.
• W2019228926 hasConceptScore W2019228926C86803240 @default.
• W2019228926 hasIssue "22" @default.
• W2019228926 hasLocation W20192289261 @default.
• W2019228926 hasLocation W20192289262 @default.
• W2019228926 hasLocation W20192289263 @default.
• W2019228926 hasLocation W20192289264 @default.
• W2019228926 hasLocation W20192289265 @default.
• W2019228926 hasLocation W20192289266 @default.
• W2019228926 hasOpenAccess W2019228926 @default.
• W2019228926 hasPrimaryLocation W20192289261 @default.
• W2019228926 hasRelatedWork W2009810460 @default.
• W2019228926 hasRelatedWork W2019228926 @default.
• W2019228926 hasRelatedWork W2062385823 @default.
• W2019228926 hasRelatedWork W2066139466 @default.

• next