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• W2019266430 abstract "Amperometry allows real-time measurement of in vivo electrochemical signals, albeit with no capacity to identify the constituents of the signal. In the present study, differential amperometry was used to monitor catecholamine and serotonin (5-HT) simultaneously at the same location. ‘Dident’ carbon fibre microelectrodes (microelectrodes with two working electrodes in a single assembly) were constructed and coated with Nafion to prevent poisoning on contact with brain tissue. One electrode (channel A) was held at +200 mV versus Ag/AgCl to monitor catecholamines selectively. This potential is too low to oxidise 5-HT. The second electrode (channel B), recording faradaic current at +500 mV versus Ag/AgCl, measured the sum of catecholamine and 5-HT oxidation. The 5-HT current component was the difference of channel B minus channel A. With appropriate balancing of the two channels, it is possible to record catecholamines and 5-HT simultaneously at the same dident microelectrode. Examples of measurements in striatum, cortex and locus coeruleus are shown." @default.
• W2019266430 created "2016-06-24" @default.
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• W2019266430 date "2004-12-01" @default.
• W2019266430 modified "2023-10-16" @default.
• W2019266430 title "Simultaneous real-time amperometric measurement of catecholamines and serotonin at carbon fibre ‘dident’ microelectrodes" @default.
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• W2019266430 doi "https://doi.org/10.1016/j.jneumeth.2004.03.023" @default.
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