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• W2019319762 abstract "A microfluidic device to quantify bacterial chemotaxis has been proposed, which generates a linear concentration gradient of chemoattractant in the main channel only by convective and molecular diffusion, and which enables the bacteria to enter the main channel in a single file by hydrodynamic focusing technique. The trajectory of each bacterium in response to the concentration gradient of chemoattractant is photographed by a CCD camera and its velocity is acquired by a simple PTV (Particle Tracking Velocimetry) algorithm. An advantage of this assay is to measure the velocity of a single bacterium and to quantify the degree of chemotaxis by analyzing the frequency of velocities concurrently. Thus, the parameter characterizing the motility of wild-type Escherichia coli strain RP437 in response to various concentration gradients of L-aspartate is obtained in such a manner that the degree of bacterial chemotaxis is quantified on the basis of a newly proposed Migration Index." @default.
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• W2019319762 date "2009-06-23" @default.
• W2019319762 modified "2023-09-26" @default.
• W2019319762 title "Quantitative analysis of single bacterial chemotaxis using a linear concentration gradient microchannel" @default.
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• W2019319762 doi "https://doi.org/10.1007/s10544-009-9330-8" @default.
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