Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019339725> ?p ?o ?g. }
- W2019339725 endingPage "97" @default.
- W2019339725 startingPage "89" @default.
- W2019339725 abstract "The anaerobic spirochete Brachyspira hyodysenteriae is host to a bacteriophage-like agent known as VSH-1. VSH-1 is a novel gene transfer mechanism which does not self-propagate and transfers random 7.5 kb fragments of host DNA between B. hyodysenteriae cells. In these investigations early events during VSH-1 induction by mitomycin C were examined. Quantitative PCR analysis revealed that VSH-1 hvp38 and hvp53 genes did not detectably increase in copy numbers during induction. Based on Northern blot hybridization assays, transcription of VSH-1 genes hvp38, hvp53, hvp45, hvp101, and lys increased fivefold to tenfold between 2 and 4 h after induction whereas mRNA levels for B. hyodysenteriae flaA1 declined over the same time period. Chloramphenicol prevented the mitomycin C-induced increases in VSH-1 gene transcription. Hydrogen peroxide (300 μM) substituted for mitomycin C as an inducer of VSH-1 gene transcription and is a possible ‘natural’ inducer of VSH-1 production in vivo. Northern blot hybridization, RT PCR, and primer extension analyses showed that VSH-1 genes are co-transcribed at an initiation site upstream of the VSH-1 gene operon. Two direct heptanucleotide repeats (ACTTATA) were identified between the putative −35 and −10 positions of the VSH-1 gene operon and are likely to represent a binding site for transcription proteins. These findings indicate VSH-1 virion production does not require genome replication, consistent with the inability of VSH-1 to self-propagate. Early events in VSH-1 induction include de novo synthesis of protein(s) essential for transcription of VSH-1 genes as polycistronic mRNA initiating upstream of the hvp45 gene." @default.
- W2019339725 created "2016-06-24" @default.
- W2019339725 creator A5008002754 @default.
- W2019339725 creator A5032927308 @default.
- W2019339725 creator A5090737757 @default.
- W2019339725 date "2007-04-01" @default.
- W2019339725 modified "2023-10-16" @default.
- W2019339725 title "Induction and transcription of VSH-1, a prophage-like gene transfer agent of Brachyspira hyodysenteriae" @default.
- W2019339725 cites W1484998934 @default.
- W2019339725 cites W1520410533 @default.
- W2019339725 cites W1593439623 @default.
- W2019339725 cites W1878196482 @default.
- W2019339725 cites W1894221274 @default.
- W2019339725 cites W1921607560 @default.
- W2019339725 cites W1969920523 @default.
- W2019339725 cites W1985757191 @default.
- W2019339725 cites W2013735086 @default.
- W2019339725 cites W2015698695 @default.
- W2019339725 cites W2017760485 @default.
- W2019339725 cites W2026255636 @default.
- W2019339725 cites W2027422709 @default.
- W2019339725 cites W2029401196 @default.
- W2019339725 cites W2030010981 @default.
- W2019339725 cites W2045902027 @default.
- W2019339725 cites W2064686642 @default.
- W2019339725 cites W2078689375 @default.
- W2019339725 cites W2083075664 @default.
- W2019339725 cites W2091562946 @default.
- W2019339725 cites W2100975834 @default.
- W2019339725 cites W2101074277 @default.
- W2019339725 cites W2101109460 @default.
- W2019339725 cites W2101511369 @default.
- W2019339725 cites W2111795956 @default.
- W2019339725 cites W2111835074 @default.
- W2019339725 cites W2112038084 @default.
- W2019339725 cites W2118832630 @default.
- W2019339725 cites W2124669173 @default.
- W2019339725 cites W2128627959 @default.
- W2019339725 cites W2128829074 @default.
- W2019339725 cites W2129492241 @default.
- W2019339725 cites W2129634213 @default.
- W2019339725 cites W2129769696 @default.
- W2019339725 cites W2133286749 @default.
- W2019339725 cites W2138433608 @default.
- W2019339725 cites W2148591264 @default.
- W2019339725 cites W2149672427 @default.
- W2019339725 cites W2150508721 @default.
- W2019339725 cites W2150927648 @default.
- W2019339725 cites W2161834179 @default.
- W2019339725 cites W2164797653 @default.
- W2019339725 cites W2168167613 @default.
- W2019339725 cites W2171054167 @default.
- W2019339725 cites W2284195039 @default.
- W2019339725 cites W941733805 @default.
- W2019339725 doi "https://doi.org/10.1016/j.anaerobe.2007.04.004" @default.
- W2019339725 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17540587" @default.
- W2019339725 hasPublicationYear "2007" @default.
- W2019339725 type Work @default.
- W2019339725 sameAs 2019339725 @default.
- W2019339725 citedByCount "16" @default.
- W2019339725 countsByYear W20193397252012 @default.
- W2019339725 countsByYear W20193397252015 @default.
- W2019339725 countsByYear W20193397252019 @default.
- W2019339725 countsByYear W20193397252020 @default.
- W2019339725 crossrefType "journal-article" @default.
- W2019339725 hasAuthorship W2019339725A5008002754 @default.
- W2019339725 hasAuthorship W2019339725A5032927308 @default.
- W2019339725 hasAuthorship W2019339725A5090737757 @default.
- W2019339725 hasConcept C101762097 @default.
- W2019339725 hasConcept C104317684 @default.
- W2019339725 hasConcept C133307689 @default.
- W2019339725 hasConcept C138885662 @default.
- W2019339725 hasConcept C150194340 @default.
- W2019339725 hasConcept C153911025 @default.
- W2019339725 hasConcept C179926584 @default.
- W2019339725 hasConcept C203075996 @default.
- W2019339725 hasConcept C2225953 @default.
- W2019339725 hasConcept C2776441376 @default.
- W2019339725 hasConcept C41895202 @default.
- W2019339725 hasConcept C54355233 @default.
- W2019339725 hasConcept C547475151 @default.
- W2019339725 hasConcept C552990157 @default.
- W2019339725 hasConcept C73445445 @default.
- W2019339725 hasConcept C86803240 @default.
- W2019339725 hasConceptScore W2019339725C101762097 @default.
- W2019339725 hasConceptScore W2019339725C104317684 @default.
- W2019339725 hasConceptScore W2019339725C133307689 @default.
- W2019339725 hasConceptScore W2019339725C138885662 @default.
- W2019339725 hasConceptScore W2019339725C150194340 @default.
- W2019339725 hasConceptScore W2019339725C153911025 @default.
- W2019339725 hasConceptScore W2019339725C179926584 @default.
- W2019339725 hasConceptScore W2019339725C203075996 @default.
- W2019339725 hasConceptScore W2019339725C2225953 @default.
- W2019339725 hasConceptScore W2019339725C2776441376 @default.
- W2019339725 hasConceptScore W2019339725C41895202 @default.
- W2019339725 hasConceptScore W2019339725C54355233 @default.
- W2019339725 hasConceptScore W2019339725C547475151 @default.
- W2019339725 hasConceptScore W2019339725C552990157 @default.