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- W2019343015 abstract "By site-directed mutagenesis we have changed the serine residue 232 of the phi 29 terminal protein, involved in the covalent linkage to dAMP for the initiation of replication, into a threonine residue. The mutant terminal protein has been purified to homogeneity and shown to be inactive in the formation of the initiation complex; nevertheless, the mutant protein retains its ability to interact with the phi 29 DNA polymerase and with the DNA. The results obtained indicate a high specificity in the linking site of the terminal protein." @default.
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- W2019343015 date "1988-07-11" @default.
- W2019343015 modified "2023-09-26" @default.
- W2019343015 title "Site-directed mutagenesis in the DNA linking site of bacteriophage o29 terminal protein: isolation and characterization of a Ser232->Thr mutant" @default.
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- W2019343015 doi "https://doi.org/10.1093/nar/16.13.5727" @default.
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