FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019343885> ?p ?o ?g. }

• W2019343885 endingPage "e88101" @default.
• W2019343885 startingPage "e88101" @default.
• W2019343885 abstract "The human cytomegalovirus (HCMV) U(L)26 gene encodes a virion protein that is important for high titer viral replication. To identify specific domains within the U(L)26 protein that contribute to viral infection, we created a panel of site-directed U(L)26 mutant viruses and assessed their impact on phenotypes attributed to U(L)26. We find that the C-terminal 38 amino acids of the U(L)26 protein are absolutely necessary for U(L)26 function. A stop-insertion mutant that produced a truncated U(L)26 protein lacking this region behaved identically to U(L)26-null viruses. This included reduced accumulation of IE1 protein at early time points, smaller plaque size, reduced virion stability, and growth with similarly attenuated kinetics. This C-terminal truncation decreased the amount of U(L)26 packaged into the virion resulting in reduced delivery of U(L)26 to newly infected cells. Further, this C-terminal truncated U(L)26 exhibited substantially reduced nuclear localization compared to wildtype U(L)26. Translation of U(L)26 mRNA is initiated from two separate in frame methionines that give rise to a long and a short isoform of U(L)26. We find that the N-terminal 34 amino acids, which are unique to the long isoform of U(L)26, are also important for the function of the U(L)26 protein. A viral mutant that produces only the short isoform of U(L)26 and lacks these N-terminal 34 amino acids exhibits delayed IE1 accumulation, and demonstrates intermediate defects in viral plaque size, virion stability and viral growth kinetics. Ablation of the short U(L)26 isoform in the presence of the long U(L)26 isoform did not impact any of the in vitro phenotypes tested. These experiments highlight important domains within the U(L)26 protein that contribute to HCMV infection." @default.
• W2019343885 created "2016-06-24" @default.
• W2019343885 creator A5007558424 @default.
• W2019343885 creator A5013537234 @default.
• W2019343885 creator A5043732452 @default.
• W2019343885 date "2014-02-05" @default.
• W2019343885 modified "2023-10-15" @default.
• W2019343885 title "Distinct Domains within the Human Cytomegalovirus UL26 Protein Are Important for Wildtype Viral Replication and Virion Stability" @default.
• W2019343885 cites W1488965868 @default.
• W2019343885 cites W1667923652 @default.
• W2019343885 cites W1746954312 @default.
• W2019343885 cites W1851333263 @default.
• W2019343885 cites W1916620382 @default.
• W2019343885 cites W1994882633 @default.
• W2019343885 cites W2003847336 @default.
• W2019343885 cites W2023880399 @default.
• W2019343885 cites W2024803868 @default.
• W2019343885 cites W2030172852 @default.
• W2019343885 cites W2033207340 @default.
• W2019343885 cites W2047276100 @default.
• W2019343885 cites W2077132385 @default.
• W2019343885 cites W2087874554 @default.
• W2019343885 cites W2094108392 @default.
• W2019343885 cites W2094904848 @default.
• W2019343885 cites W2100195610 @default.
• W2019343885 cites W2110429239 @default.
• W2019343885 cites W2114858538 @default.
• W2019343885 cites W2125061016 @default.
• W2019343885 cites W2125451710 @default.
• W2019343885 cites W2128570864 @default.
• W2019343885 cites W2131468538 @default.
• W2019343885 cites W2135432766 @default.
• W2019343885 cites W2136913046 @default.
• W2019343885 cites W2140436032 @default.
• W2019343885 cites W2142337180 @default.
• W2019343885 cites W2145469304 @default.
• W2019343885 cites W2153924587 @default.
• W2019343885 cites W2158046192 @default.
• W2019343885 cites W2169900672 @default.
• W2019343885 doi "https://doi.org/10.1371/journal.pone.0088101" @default.
• W2019343885 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3914908" @default.
• W2019343885 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24505393" @default.
• W2019343885 hasPublicationYear "2014" @default.
• W2019343885 type Work @default.
• W2019343885 sameAs 2019343885 @default.
• W2019343885 citedByCount "11" @default.
• W2019343885 countsByYear W20193438852014 @default.
• W2019343885 countsByYear W20193438852016 @default.
• W2019343885 countsByYear W20193438852018 @default.
• W2019343885 countsByYear W20193438852019 @default.
• W2019343885 countsByYear W20193438852021 @default.
• W2019343885 countsByYear W20193438852023 @default.
• W2019343885 crossrefType "journal-article" @default.
• W2019343885 hasAuthorship W2019343885A5007558424 @default.
• W2019343885 hasAuthorship W2019343885A5013537234 @default.
• W2019343885 hasAuthorship W2019343885A5043732452 @default.
• W2019343885 hasBestOaLocation W20193438851 @default.
• W2019343885 hasConcept C104317684 @default.
• W2019343885 hasConcept C140704245 @default.
• W2019343885 hasConcept C143065580 @default.
• W2019343885 hasConcept C153911025 @default.
• W2019343885 hasConcept C159047783 @default.
• W2019343885 hasConcept C207583985 @default.
• W2019343885 hasConcept C2522874641 @default.
• W2019343885 hasConcept C2779820661 @default.
• W2019343885 hasConcept C2779926675 @default.
• W2019343885 hasConcept C2780879997 @default.
• W2019343885 hasConcept C515207424 @default.
• W2019343885 hasConcept C53345823 @default.
• W2019343885 hasConcept C54355233 @default.
• W2019343885 hasConcept C86803240 @default.
• W2019343885 hasConceptScore W2019343885C104317684 @default.
• W2019343885 hasConceptScore W2019343885C140704245 @default.
• W2019343885 hasConceptScore W2019343885C143065580 @default.
• W2019343885 hasConceptScore W2019343885C153911025 @default.
• W2019343885 hasConceptScore W2019343885C159047783 @default.
• W2019343885 hasConceptScore W2019343885C207583985 @default.
• W2019343885 hasConceptScore W2019343885C2522874641 @default.
• W2019343885 hasConceptScore W2019343885C2779820661 @default.
• W2019343885 hasConceptScore W2019343885C2779926675 @default.
• W2019343885 hasConceptScore W2019343885C2780879997 @default.
• W2019343885 hasConceptScore W2019343885C515207424 @default.
• W2019343885 hasConceptScore W2019343885C53345823 @default.
• W2019343885 hasConceptScore W2019343885C54355233 @default.
• W2019343885 hasConceptScore W2019343885C86803240 @default.
• W2019343885 hasIssue "2" @default.
• W2019343885 hasLocation W20193438851 @default.
• W2019343885 hasLocation W20193438852 @default.
• W2019343885 hasLocation W20193438853 @default.
• W2019343885 hasLocation W20193438854 @default.
• W2019343885 hasOpenAccess W2019343885 @default.
• W2019343885 hasPrimaryLocation W20193438851 @default.
• W2019343885 hasRelatedWork W1554885052 @default.
• W2019343885 hasRelatedWork W2019343885 @default.
• W2019343885 hasRelatedWork W2033005386 @default.
• W2019343885 hasRelatedWork W2033712814 @default.
• W2019343885 hasRelatedWork W2083117756 @default.
• W2019343885 hasRelatedWork W2125365865 @default.

• next