FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019352625> ?p ?o ?g. }

• W2019352625 endingPage "e61985" @default.
• W2019352625 startingPage "e61985" @default.
• W2019352625 abstract "Laccases are highly stable, industrially important enzymes capable of oxidizing a large range of substrates. Causes for their stability are, as for other proteins, poorly understood. In this work, multiple-seed molecular dynamics (MD) was applied to a Trametes versicolor laccase in response to variable ionic strengths, temperatures, and glycosylation status. Near-physiological conditions provided excellent agreement with the crystal structure (average RMSD ∼0.92 Å) and residual agreement with experimental B-factors. The persistence of backbone hydrogen bonds was identified as a key descriptor of structural response to environment, whereas solvent-accessibility, radius of gyration, and fluctuations were only locally relevant. Backbone hydrogen bonds decreased systematically with temperature in all simulations (∼9 per 50 K), probing structural changes associated with enthalpy-entropy compensation. Approaching Topt (∼350 K) from 300 K, this change correlated with a beginning “unzipping” of critical β-sheets. 0 M ionic strength triggered partial denucleation of the C-terminal (known experimentally to be sensitive) at 400 K, suggesting a general salt stabilization effect. In contrast, F− (but not Cl−) specifically impaired secondary structure by formation of strong hydrogen bonds with backbone NH, providing a mechanism for experimentally observed small anion destabilization, potentially remedied by site-directed mutagenesis at critical intrusion sites. N-glycosylation was found to support structural integrity by increasing persistent backbone hydrogen bonds by ∼4 across simulations, mainly via prevention of F− intrusion. Hydrogen-bond loss in distinct loop regions and ends of critical β-sheets suggest potential strategies for laboratory optimization of these industrially important enzymes." @default.
• W2019352625 created "2016-06-24" @default.
• W2019352625 creator A5035519916 @default.
• W2019352625 creator A5084810235 @default.
• W2019352625 date "2013-04-29" @default.
• W2019352625 modified "2023-10-01" @default.
• W2019352625 title "Stability Mechanisms of a Thermophilic Laccase Probed by Molecular Dynamics" @default.
• W2019352625 cites W1846327373 @default.
• W2019352625 cites W1965458294 @default.
• W2019352625 cites W1971900847 @default.
• W2019352625 cites W1972464599 @default.
• W2019352625 cites W1972758026 @default.
• W2019352625 cites W1973347726 @default.
• W2019352625 cites W1973505268 @default.
• W2019352625 cites W1975588280 @default.
• W2019352625 cites W1976499671 @default.
• W2019352625 cites W1980316342 @default.
• W2019352625 cites W1985766158 @default.
• W2019352625 cites W1987648856 @default.
• W2019352625 cites W1991101438 @default.
• W2019352625 cites W1991193821 @default.
• W2019352625 cites W1992029173 @default.
• W2019352625 cites W1995730663 @default.
• W2019352625 cites W1996350754 @default.
• W2019352625 cites W2001264094 @default.
• W2019352625 cites W2003890287 @default.
• W2019352625 cites W2004629624 @default.
• W2019352625 cites W2008819364 @default.
• W2019352625 cites W2010965811 @default.
• W2019352625 cites W2012907468 @default.
• W2019352625 cites W2015006592 @default.
• W2019352625 cites W2017196167 @default.
• W2019352625 cites W2017835622 @default.
• W2019352625 cites W2018810660 @default.
• W2019352625 cites W2023579027 @default.
• W2019352625 cites W2023686356 @default.
• W2019352625 cites W2026822031 @default.
• W2019352625 cites W2028161249 @default.
• W2019352625 cites W2028291957 @default.
• W2019352625 cites W2029667189 @default.
• W2019352625 cites W2035266068 @default.
• W2019352625 cites W2035687084 @default.
• W2019352625 cites W2036502756 @default.
• W2019352625 cites W2045608551 @default.
• W2019352625 cites W2051927163 @default.
• W2019352625 cites W2052615119 @default.
• W2019352625 cites W2052677774 @default.
• W2019352625 cites W2058206198 @default.
• W2019352625 cites W2060816361 @default.
• W2019352625 cites W2066196541 @default.
• W2019352625 cites W2066988778 @default.
• W2019352625 cites W2079963456 @default.
• W2019352625 cites W2093221728 @default.
• W2019352625 cites W2096004249 @default.
• W2019352625 cites W2100583429 @default.
• W2019352625 cites W2104176629 @default.
• W2019352625 cites W2106594981 @default.
• W2019352625 cites W2107065107 @default.
• W2019352625 cites W2107150350 @default.
• W2019352625 cites W2110156926 @default.
• W2019352625 cites W2111804569 @default.
• W2019352625 cites W2120230791 @default.
• W2019352625 cites W2121437914 @default.
• W2019352625 cites W2124024967 @default.
• W2019352625 cites W2127830286 @default.
• W2019352625 cites W2130479394 @default.
• W2019352625 cites W2133573415 @default.
• W2019352625 cites W2133641393 @default.
• W2019352625 cites W2139239540 @default.
• W2019352625 cites W2141920771 @default.
• W2019352625 cites W2146890885 @default.
• W2019352625 cites W2154486038 @default.
• W2019352625 cites W2154665416 @default.
• W2019352625 cites W2158518946 @default.
• W2019352625 cites W2158543301 @default.
• W2019352625 cites W2158770580 @default.
• W2019352625 cites W2162505394 @default.
• W2019352625 cites W2163408172 @default.
• W2019352625 cites W2167279962 @default.
• W2019352625 cites W2179033920 @default.
• W2019352625 cites W2165432973 @default.
• W2019352625 doi "https://doi.org/10.1371/journal.pone.0061985" @default.
• W2019352625 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3639223" @default.
• W2019352625 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23658618" @default.
• W2019352625 hasPublicationYear "2013" @default.
• W2019352625 type Work @default.
• W2019352625 sameAs 2019352625 @default.
• W2019352625 citedByCount "45" @default.
• W2019352625 countsByYear W20193526252013 @default.
• W2019352625 countsByYear W20193526252014 @default.
• W2019352625 countsByYear W20193526252015 @default.
• W2019352625 countsByYear W20193526252016 @default.
• W2019352625 countsByYear W20193526252017 @default.
• W2019352625 countsByYear W20193526252018 @default.
• W2019352625 countsByYear W20193526252019 @default.
• W2019352625 countsByYear W20193526252020 @default.
• W2019352625 countsByYear W20193526252021 @default.
• W2019352625 countsByYear W20193526252022 @default.

• next