Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019352743> ?p ?o ?g. }
- W2019352743 endingPage "2300" @default.
- W2019352743 startingPage "2293" @default.
- W2019352743 abstract "The tetrameric plus-end-directed motor, kinesin-5, is essential for bipolar spindle assembly. Small-molecule inhibitors of kinesin-5 have been important tools for investigating its function, and some are currently under evaluation as anti-cancer drugs. Most inhibitors reported to date are ;non-competitive' and bind to a specific site on the motor head, trapping the motor in an ADP-bound state in which it has a weak but non-zero affinity for microtubules. Here, we used a novel ATP-competitive inhibitor, FCPT, developed at Merck (USA). We found that it induced tight binding of kinesin-5 onto microtubules in vitro. Using Xenopus egg-extract spindles, we found that FCPT not only blocked poleward microtubule sliding but also selectively induced loss of microtubules at the poles of bipolar spindles (and not asters or monoasters). We also found that the spindle-pole proteins TPX2 and gamma-tubulin became redistributed to the spindle equator, suggesting that proper kinesin-5 function is required for pole assembly." @default.
- W2019352743 created "2016-06-24" @default.
- W2019352743 creator A5013969453 @default.
- W2019352743 creator A5032467861 @default.
- W2019352743 creator A5042735526 @default.
- W2019352743 creator A5048665713 @default.
- W2019352743 creator A5059124650 @default.
- W2019352743 creator A5067096193 @default.
- W2019352743 creator A5085261321 @default.
- W2019352743 date "2008-07-15" @default.
- W2019352743 modified "2023-09-23" @default.
- W2019352743 title "A novel small-molecule inhibitor reveals a possible role of kinesin-5 in anastral spindle-pole assembly" @default.
- W2019352743 cites W1507086201 @default.
- W2019352743 cites W1801796326 @default.
- W2019352743 cites W1970795083 @default.
- W2019352743 cites W1985701763 @default.
- W2019352743 cites W1991690400 @default.
- W2019352743 cites W2004568111 @default.
- W2019352743 cites W2006069089 @default.
- W2019352743 cites W2006876135 @default.
- W2019352743 cites W2019912010 @default.
- W2019352743 cites W2027532431 @default.
- W2019352743 cites W2029178312 @default.
- W2019352743 cites W2031487892 @default.
- W2019352743 cites W2042901082 @default.
- W2019352743 cites W2045518803 @default.
- W2019352743 cites W2048467789 @default.
- W2019352743 cites W2053535892 @default.
- W2019352743 cites W2075081297 @default.
- W2019352743 cites W2076659391 @default.
- W2019352743 cites W2081721965 @default.
- W2019352743 cites W2081879298 @default.
- W2019352743 cites W2085102956 @default.
- W2019352743 cites W2099687412 @default.
- W2019352743 cites W2100649932 @default.
- W2019352743 cites W2101176165 @default.
- W2019352743 cites W2108376231 @default.
- W2019352743 cites W2108422673 @default.
- W2019352743 cites W2111827360 @default.
- W2019352743 cites W2118218174 @default.
- W2019352743 cites W2121132479 @default.
- W2019352743 cites W2128350011 @default.
- W2019352743 cites W2131938193 @default.
- W2019352743 cites W2133492742 @default.
- W2019352743 cites W2142853609 @default.
- W2019352743 cites W2143708956 @default.
- W2019352743 cites W2145131593 @default.
- W2019352743 cites W2146314877 @default.
- W2019352743 cites W2149249386 @default.
- W2019352743 cites W2157151675 @default.
- W2019352743 cites W2157253543 @default.
- W2019352743 cites W2164962038 @default.
- W2019352743 cites W2170801143 @default.
- W2019352743 cites W2398705786 @default.
- W2019352743 cites W4231860297 @default.
- W2019352743 cites W935855719 @default.
- W2019352743 doi "https://doi.org/10.1242/jcs.024018" @default.
- W2019352743 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2902979" @default.
- W2019352743 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18559893" @default.
- W2019352743 hasPublicationYear "2008" @default.
- W2019352743 type Work @default.
- W2019352743 sameAs 2019352743 @default.
- W2019352743 citedByCount "50" @default.
- W2019352743 countsByYear W20193527432012 @default.
- W2019352743 countsByYear W20193527432013 @default.
- W2019352743 countsByYear W20193527432014 @default.
- W2019352743 countsByYear W20193527432015 @default.
- W2019352743 countsByYear W20193527432016 @default.
- W2019352743 countsByYear W20193527432017 @default.
- W2019352743 countsByYear W20193527432018 @default.
- W2019352743 countsByYear W20193527432019 @default.
- W2019352743 countsByYear W20193527432020 @default.
- W2019352743 countsByYear W20193527432021 @default.
- W2019352743 countsByYear W20193527432022 @default.
- W2019352743 crossrefType "journal-article" @default.
- W2019352743 hasAuthorship W2019352743A5013969453 @default.
- W2019352743 hasAuthorship W2019352743A5032467861 @default.
- W2019352743 hasAuthorship W2019352743A5042735526 @default.
- W2019352743 hasAuthorship W2019352743A5048665713 @default.
- W2019352743 hasAuthorship W2019352743A5059124650 @default.
- W2019352743 hasAuthorship W2019352743A5067096193 @default.
- W2019352743 hasAuthorship W2019352743A5085261321 @default.
- W2019352743 hasBestOaLocation W20193527432 @default.
- W2019352743 hasConcept C104317684 @default.
- W2019352743 hasConcept C12554922 @default.
- W2019352743 hasConcept C1491633281 @default.
- W2019352743 hasConcept C160408235 @default.
- W2019352743 hasConcept C20418707 @default.
- W2019352743 hasConcept C2779535977 @default.
- W2019352743 hasConcept C47432576 @default.
- W2019352743 hasConcept C55493867 @default.
- W2019352743 hasConcept C59006786 @default.
- W2019352743 hasConcept C70547292 @default.
- W2019352743 hasConcept C84425145 @default.
- W2019352743 hasConcept C85813293 @default.
- W2019352743 hasConcept C86803240 @default.
- W2019352743 hasConcept C93126451 @default.
- W2019352743 hasConcept C93304396 @default.