Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019379615> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2019379615 endingPage "125" @default.
- W2019379615 startingPage "120" @default.
- W2019379615 abstract "Current methods for multiparameter DNA flow cytometry suffer from several limitations. These include significant modifications of cell morphological parameters, the impossibility to counterstain cells with certain fluorochromes, and laborious tuning of the instrument that, for some procedures, must be equipped with an ultraviolet (UV) laser. To overcome these problems, we developed a novel method for the simultaneous analysis of morphological parameters, four-color immunophenotyping, and stoichiometric DNA labeling using a bench-top flow cytometer.The method consists of a mild permeabilization/fixation treatment at room temperature, followed by labeling with fluorochrome-conjugated monoclonal antibodies (mAbs) and with the DNA dye 7-aminoactinomycin D (7-AAD) at 56 degrees C.Using this method, we analyzed resting peripheral blood mononucleated cells (PBMC), proliferating T cells cultured in the presence of interleukin-2 (IL-2), and lymphoblastoid B cells. Lymphocytes, monocytes, and lymphoblasts treated by this procedure retained differential light scattering (DLS) characteristics virtually identical to those of untreated cells. This allowed regions to be drawn on forward scatter (FSC) and side scatter (SSC) cytograms resolving different cell populations. DLS were preserved well enough to distinguish large lymphoblasts in the S or G2/M phases from small G0/G1 cells. Also, stainability with fluorescein-isothiocyanate (FITC), R-phycoerythrin (PE), allophycocyanin (APC)-conjugated mAbs was generally preserved. DNA labeling with 7-AAD was of quality good enough to permit accurate cell cycle analysis.The method described here, which we called integral hot staining (IHS), represents a very simple, reproducible, and conservative assay for multiparameter DNA analysis using a bench-top flow cytometer. Last but not least, the cytometer tuning for multiparameter acquisition is straightforward." @default.
- W2019379615 created "2016-06-24" @default.
- W2019379615 creator A5032466668 @default.
- W2019379615 creator A5073605095 @default.
- W2019379615 creator A5086838454 @default.
- W2019379615 date "2001-01-01" @default.
- W2019379615 modified "2023-09-27" @default.
- W2019379615 title "A unified procedure for conservative (morphology) and integral (DNA and immunophenotype) cell staining for flow cytometry" @default.
- W2019379615 cites W1552946383 @default.
- W2019379615 cites W2000667599 @default.
- W2019379615 cites W2017753207 @default.
- W2019379615 cites W2021706671 @default.
- W2019379615 cites W2025028340 @default.
- W2019379615 cites W2036284007 @default.
- W2019379615 cites W2036937264 @default.
- W2019379615 cites W2043206546 @default.
- W2019379615 cites W2054938881 @default.
- W2019379615 cites W2059500251 @default.
- W2019379615 cites W2074810026 @default.
- W2019379615 cites W2135438467 @default.
- W2019379615 cites W2331653775 @default.
- W2019379615 cites W2410915101 @default.
- W2019379615 cites W3158889629 @default.
- W2019379615 doi "https://doi.org/10.1002/1097-0320(20010601)44:2<120::aid-cyto1090>3.0.co;2-7" @default.
- W2019379615 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11378862" @default.
- W2019379615 hasPublicationYear "2001" @default.
- W2019379615 type Work @default.
- W2019379615 sameAs 2019379615 @default.
- W2019379615 citedByCount "19" @default.
- W2019379615 countsByYear W20193796152014 @default.
- W2019379615 countsByYear W20193796152015 @default.
- W2019379615 countsByYear W20193796152016 @default.
- W2019379615 countsByYear W20193796152020 @default.
- W2019379615 countsByYear W20193796152021 @default.
- W2019379615 crossrefType "journal-article" @default.
- W2019379615 hasAuthorship W2019379615A5032466668 @default.
- W2019379615 hasAuthorship W2019379615A5073605095 @default.
- W2019379615 hasAuthorship W2019379615A5086838454 @default.
- W2019379615 hasBestOaLocation W20193796151 @default.
- W2019379615 hasConcept C113842279 @default.
- W2019379615 hasConcept C120665830 @default.
- W2019379615 hasConcept C121332964 @default.
- W2019379615 hasConcept C153911025 @default.
- W2019379615 hasConcept C196166836 @default.
- W2019379615 hasConcept C2777019933 @default.
- W2019379615 hasConcept C2778093064 @default.
- W2019379615 hasConcept C2778793157 @default.
- W2019379615 hasConcept C2779669040 @default.
- W2019379615 hasConcept C2780339063 @default.
- W2019379615 hasConcept C2781294515 @default.
- W2019379615 hasConcept C523546767 @default.
- W2019379615 hasConcept C54355233 @default.
- W2019379615 hasConcept C553184892 @default.
- W2019379615 hasConcept C74864618 @default.
- W2019379615 hasConcept C81885089 @default.
- W2019379615 hasConcept C86803240 @default.
- W2019379615 hasConcept C91881484 @default.
- W2019379615 hasConceptScore W2019379615C113842279 @default.
- W2019379615 hasConceptScore W2019379615C120665830 @default.
- W2019379615 hasConceptScore W2019379615C121332964 @default.
- W2019379615 hasConceptScore W2019379615C153911025 @default.
- W2019379615 hasConceptScore W2019379615C196166836 @default.
- W2019379615 hasConceptScore W2019379615C2777019933 @default.
- W2019379615 hasConceptScore W2019379615C2778093064 @default.
- W2019379615 hasConceptScore W2019379615C2778793157 @default.
- W2019379615 hasConceptScore W2019379615C2779669040 @default.
- W2019379615 hasConceptScore W2019379615C2780339063 @default.
- W2019379615 hasConceptScore W2019379615C2781294515 @default.
- W2019379615 hasConceptScore W2019379615C523546767 @default.
- W2019379615 hasConceptScore W2019379615C54355233 @default.
- W2019379615 hasConceptScore W2019379615C553184892 @default.
- W2019379615 hasConceptScore W2019379615C74864618 @default.
- W2019379615 hasConceptScore W2019379615C81885089 @default.
- W2019379615 hasConceptScore W2019379615C86803240 @default.
- W2019379615 hasConceptScore W2019379615C91881484 @default.
- W2019379615 hasIssue "2" @default.
- W2019379615 hasLocation W20193796151 @default.
- W2019379615 hasLocation W20193796152 @default.
- W2019379615 hasOpenAccess W2019379615 @default.
- W2019379615 hasPrimaryLocation W20193796151 @default.
- W2019379615 hasRelatedWork W1993929633 @default.
- W2019379615 hasRelatedWork W2016451388 @default.
- W2019379615 hasRelatedWork W2019379615 @default.
- W2019379615 hasRelatedWork W2065989394 @default.
- W2019379615 hasRelatedWork W2075588202 @default.
- W2019379615 hasRelatedWork W2354762939 @default.
- W2019379615 hasRelatedWork W2381641348 @default.
- W2019379615 hasRelatedWork W2762695977 @default.
- W2019379615 hasRelatedWork W4297474497 @default.
- W2019379615 hasRelatedWork W4319432312 @default.
- W2019379615 hasVolume "44" @default.
- W2019379615 isParatext "false" @default.
- W2019379615 isRetracted "false" @default.
- W2019379615 magId "2019379615" @default.
- W2019379615 workType "article" @default.