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- W2019401761 abstract "Jackbean urease was immobilized on two clay minerals (kaolinite and montmorillonite) and the mineral constituents of two Iowa surface soils after silanization of the support surfaces by 3-aminopropyltriethoxysilane and derivitation with glutaraldehyde. Results showed that the amounts of urease immobilized decreased in the order: montmorillonite > kaolinite > Tama soil > Lester soil. The amounts of urease required for maximum immobilization were 70, 90, 30 and 70 mg g−1 of kaolinite, montmorillonite, Tama soil and Lester soil, respectively. The optimal pH value of immobilized urease, except that on montmorillonite (8.5), was similar to that of free urease (7.0) and it was lower than those of native soil urease. The Km values of immobilized urease (25.1–60.8 mM) were in the same order of magnitude as that of free urease (29.4 mM) but one order of magnitude higher than those of soil urease (1.77–2.90 mM). Optimal temperature of immobilized urease (60°C) was the same as that for free urease but was lower than those of soil urease (70°C). Ea and ΔHa values of urease immobilized on kaolinite and on soil mineral constituents were similar to those of the free enzyme but were lower than those of native soil urease. Immobilization of urease on clay surfaces lead to increases in the kinetic constants." @default.
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- W2019401761 title "Kinetic parameters of immobilized urease" @default.
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- W2019401761 doi "https://doi.org/10.1016/0038-0717(92)90222-j" @default.
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