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- W2019413310 abstract "This study attempted to identify a possible antibody response toHelicobacter pylori,which is associated with patients with adeno-carcinoma of the stomach. By using proteins ofH. pylorias the antigen, pooled sera from gastric cancer and non-cancer patients were used as the first antibody for Western blot analysis. Antibody responses to a 26 kD secreted protein were observed in pooled cancer sera, but not in pooled sera from non-cancer patients. The protein was purified, while amino acid sequences revealed that it was aH. pylorispecies specific protein. The gene of this protein was cloned and a recombinant protein was expressed inE. coli.In addition, an antibody to the recombinant protein was tested in each individual patient using Western blot analysis. None of the forty non-gastric cancer patients were positive for the antibody to the recombinantly expressed 26kD species specific protein. Meanwhile, six of the twenty four cancer patients tested positive (0/40 vs 6/24, p<0.01). Results presented herein demonstrate that the species specific protein ofH. pylorican be useful in detectingH.pyloriassociated with adenocarcinoma of the stomach." @default.
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- W2019413310 date "1998-03-01" @default.
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- W2019413310 title "Antibody to aHelicobacter pyloriSpecies Specific Antigen in Patients with Adenocarcinoma of the Stomach" @default.
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- W2019413310 doi "https://doi.org/10.1006/bbrc.1998.8271" @default.
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