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• W2019414865 abstract "[2-3H]Estradiol was synthesized from 2-iodoestradiol by reduction with sodium borotritiride in the presence of palladium chloride as a catalyst. When the labeled substrate was incubated with the rat liver and kidney microsomes, the tritium label was liberated quantitatively depending upon the 2-hydroxylase activity. Tritiated water produced in the incubation medium was recovered as a satisfactory rate by passage through a column of Amberlite XAD-2 resin without any interference due to the labeled substrate. The present method for the assay of estradiol 2-hydroxylase activity was found to be simple, reliable, and sensitive (detection limit:29 pmol of 2-hydroxyestradiol)." @default.
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• W2019414865 date "1980-05-01" @default.
• W2019414865 modified "2023-09-23" @default.
• W2019414865 title "A simple radiometric assay for estradiol 2-hydroxylase activity" @default.
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• W2019414865 doi "https://doi.org/10.1016/0003-2697(80)90077-9" @default.
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