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- W2019422007 abstract "OBJECTIVE: Hypoxia is a main trigger in the development of post-operative adhesions. Exposure of normal peritoneal fibroblasts to hypoxia induces the adhesion phenotype, which is characterized by increased levels of TGFβ1 and Collagen-I (Col-I). Hypoxia exerts its effects, in part by, a HIF1α-dependent mechanism. The role of macrophages in the development of the adhesion phenotype is not well understood. Therefore, the objective of this study is to determine whether hypoxia induces the expression of TGFβ1 in human macrophages in a mechanism involving HIF1α. DESIGN: University Research Laboratory MATERIALS AND METHODS: Real-time reverse transcriptase polymerase chain reaction (real-time RT/PCR) and small interfering RNA (siRNA) technology were utilized to determine TGFβ1 gene expression in macrophages under hypoxic conditions. Macrophages were cultured under normal (20% O2) and hypoxic (2% O2) conditions with or without silencing HIF1α gene expression by utilizing specific HIF1α siRNA. Total RNA extracted from each group was subjected to real-time RT/PCR to measure HIF-1α and TGFβ1 mRNA levels. Total RNA from each group was extracted from each group and complimentary DNA (cDNA) was synthesized. Aliquots of cDNA were subjected to real-time RT/PCR to determine mRNA levels for TGFβ1 and HIF1α. RESULTS: Hypoxia resulted in a significant increase in both HIF-1α (from 25.91 to 31.78 pg HIF-1α/mg RNA; p < 0.05) and TGFβ1 (from 8.79 pg to 16.33 pg TGF-β1/mg RNA; p < 0.05) mRNA levels in macrophages before and after hypoxia treatment. Silencing HIF-1α gene expression resulted in a significant decrease in HIF-1α (from 31.80 pg to 8.38 pg HIF-1α/mg RNA; p < 0.05) and TGF-β1 (from 16.33 to 5.30 pg TGF-β1/mg RNA; p < 0.05) under hypoxic conditions. CONCLUSIONS: This study indicates that human macrophages express TGFβ1 and that this expression is induced by hypoxia through a HIF-1α-dependent mechanism." @default.
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- W2019422007 date "2009-09-01" @default.
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- W2019422007 title "Hypoxia induces transforming growth factor beta 1 (TGFβ1) in human macrophages through a hypoxia inducible factor 1α (HIF-1α) – dependent mechanism" @default.
- W2019422007 doi "https://doi.org/10.1016/j.fertnstert.2009.07.1150" @default.
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