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- W2019427653 abstract "Binding characteristics of alpha 1-adrenoceptors in the bovine prostate were examined using a radioligand binding assay. [3H]Bunazosin was used as a radioligand. The optimal incubation conditions for the radioligand binding assay were determined: incubation time (30 min), protein concentration (0.2 mg/tube) and temperature (30 degrees C). Scatchard plots for alpha 1-adrenoceptors in bovine prostates were linear and the Kd and Bmax values were 0.61 nM and 49.8 fmol/mg protein, respectively. The pKi value of terazosin was significantly higher than those of prazosin, bunazosin or phentolamine in the bovine prostate. Since this radioligand binding assay using [3H]bunazosin demonstrated the presence of alpha 1-adrenoceptors in bovine prostates, this method is useful for the assessment of alpha 1-blockers of new chemicals synthesized for the treatment of the benign prostatic hyperplasia." @default.
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- W2019427653 date "1991-01-01" @default.
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- W2019427653 title "Binding Characteristics of .ALPHA.1-Adrenoceptors in Bovine Prostate Using a Radioligand Binding Assay." @default.
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- W2019427653 doi "https://doi.org/10.1248/bpb1978.14.315" @default.
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