FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019430552> ?p ?o ?g. }

• W2019430552 endingPage "1789" @default.
• W2019430552 startingPage "1780" @default.
• W2019430552 abstract "To assess defects in expression of critical cell cycle checkpoint genes and proteins in patients with rheumatoid arthritis (RA) relative to presence or absence of methotrexate (MTX) treatment, and to investigate the role of JNK in induction of these genes by MTX.Flow cytometric analysis was used to quantify changes in levels of intracellular proteins, measure reactive oxygen species (ROS), and determine apoptosis in different lymphoid populations. Quantitative reverse transcription-polymerase chain reaction was used to identify changes in cell cycle checkpoint target genes.RA patients expressed reduced baseline levels of MAPK9, TP53, CDKN1A, CDKN1B, CHEK2, and RANGAP1 messenger RNA (mRNA) and JNK total protein. The reduction in expression of mRNA for MAPK9, TP53, CDKN1A, and CDKN1B was greater in patients not receiving MTX than in those receiving low-dose MTX, with no difference in expression levels of CHEK2 and RANGAP1 mRNA between MTX-treated and non-MTX-treated patients. Further, JNK levels were inversely correlated with C-reactive protein levels in RA patients. In tissue culture, MTX induced expression of both p53 and p21 by JNK-2- and JNK-1-dependent mechanisms, respectively, while CHEK2 and RANGAP1 were not induced by MTX. MTX also induced ROS production, JNK activation, and sensitivity to apoptosis in activated T cells. Supplementation with tetrahydrobiopterin blocked these MTX-mediated effects.Our findings support the notion that MTX restores some, but not all, of the proteins contributing to cell cycle checkpoint deficiencies in RA T cells, via a JNK-dependent pathway." @default.
• W2019430552 created "2016-06-24" @default.
• W2019430552 creator A5007263617 @default.
• W2019430552 creator A5026728364 @default.
• W2019430552 creator A5035534167 @default.
• W2019430552 creator A5036914251 @default.
• W2019430552 creator A5081455159 @default.
• W2019430552 date "2012-05-25" @default.
• W2019430552 modified "2023-09-23" @default.
• W2019430552 title "Methotrexate increases expression of cell cycle checkpoint genes via JNK activation" @default.
• W2019430552 cites W1485133278 @default.
• W2019430552 cites W1970037245 @default.
• W2019430552 cites W1971035689 @default.
• W2019430552 cites W1975849817 @default.
• W2019430552 cites W1976445136 @default.
• W2019430552 cites W1985099044 @default.
• W2019430552 cites W1988876917 @default.
• W2019430552 cites W1989089149 @default.
• W2019430552 cites W1991221096 @default.
• W2019430552 cites W2008117987 @default.
• W2019430552 cites W2015228675 @default.
• W2019430552 cites W2015519364 @default.
• W2019430552 cites W2028044172 @default.
• W2019430552 cites W2029819461 @default.
• W2019430552 cites W2030232089 @default.
• W2019430552 cites W2039568202 @default.
• W2019430552 cites W2041154353 @default.
• W2019430552 cites W2054888624 @default.
• W2019430552 cites W2058631780 @default.
• W2019430552 cites W2092104789 @default.
• W2019430552 cites W2092514440 @default.
• W2019430552 cites W2096528849 @default.
• W2019430552 cites W2097980836 @default.
• W2019430552 cites W2113206255 @default.
• W2019430552 cites W2118270386 @default.
• W2019430552 cites W2127532898 @default.
• W2019430552 cites W2128952811 @default.
• W2019430552 cites W2131262676 @default.
• W2019430552 cites W2133853290 @default.
• W2019430552 cites W2136046634 @default.
• W2019430552 cites W2136542008 @default.
• W2019430552 cites W2138510167 @default.
• W2019430552 cites W2142026383 @default.
• W2019430552 cites W2145200068 @default.
• W2019430552 cites W2146304788 @default.
• W2019430552 cites W2146828787 @default.
• W2019430552 cites W2148399857 @default.
• W2019430552 cites W2149954943 @default.
• W2019430552 cites W2151081127 @default.
• W2019430552 cites W2157605608 @default.
• W2019430552 cites W2328523155 @default.
• W2019430552 cites W2328999771 @default.
• W2019430552 cites W2331320446 @default.
• W2019430552 cites W2335332412 @default.
• W2019430552 cites W4235790631 @default.
• W2019430552 cites W4294350064 @default.
• W2019430552 doi "https://doi.org/10.1002/art.34342" @default.
• W2019430552 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3310965" @default.
• W2019430552 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22183962" @default.
• W2019430552 hasPublicationYear "2012" @default.
• W2019430552 type Work @default.
• W2019430552 sameAs 2019430552 @default.
• W2019430552 citedByCount "43" @default.
• W2019430552 countsByYear W20194305522013 @default.
• W2019430552 countsByYear W20194305522014 @default.
• W2019430552 countsByYear W20194305522015 @default.
• W2019430552 countsByYear W20194305522016 @default.
• W2019430552 countsByYear W20194305522017 @default.
• W2019430552 countsByYear W20194305522018 @default.
• W2019430552 countsByYear W20194305522019 @default.
• W2019430552 countsByYear W20194305522020 @default.
• W2019430552 countsByYear W20194305522021 @default.
• W2019430552 countsByYear W20194305522022 @default.
• W2019430552 countsByYear W20194305522023 @default.
• W2019430552 crossrefType "journal-article" @default.
• W2019430552 hasAuthorship W2019430552A5007263617 @default.
• W2019430552 hasAuthorship W2019430552A5026728364 @default.
• W2019430552 hasAuthorship W2019430552A5035534167 @default.
• W2019430552 hasAuthorship W2019430552A5036914251 @default.
• W2019430552 hasAuthorship W2019430552A5081455159 @default.
• W2019430552 hasBestOaLocation W20194305522 @default.
• W2019430552 hasConcept C104317684 @default.
• W2019430552 hasConcept C105696609 @default.
• W2019430552 hasConcept C150194340 @default.
• W2019430552 hasConcept C153911025 @default.
• W2019430552 hasConcept C184235292 @default.
• W2019430552 hasConcept C185592680 @default.
• W2019430552 hasConcept C190283241 @default.
• W2019430552 hasConcept C29537977 @default.
• W2019430552 hasConcept C502942594 @default.
• W2019430552 hasConcept C55493867 @default.
• W2019430552 hasConcept C86803240 @default.
• W2019430552 hasConcept C95444343 @default.
• W2019430552 hasConceptScore W2019430552C104317684 @default.
• W2019430552 hasConceptScore W2019430552C105696609 @default.
• W2019430552 hasConceptScore W2019430552C150194340 @default.
• W2019430552 hasConceptScore W2019430552C153911025 @default.
• W2019430552 hasConceptScore W2019430552C184235292 @default.

• next