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- W2019430912 abstract "The carotid body of the mouse is ideally suited for in vitro photometric and fluorometric studies using transillumination. It is small, thin and translucent. Photometric recordings were made by using a dual wavelength method. Wavelengths of 550 nm (maximal absorbance, MA) and 540 nm (isosbestic point, IP) were employed to study cytochromec, 600 nm (MA) and 620 nm (IP) for a pigment presumed to be cytochromeaa3, 580 nm (MA) and 570 or 542 nm (IP) tp control for possible effects of hemoglobin (Hb). Exposure of the organ to NaCN (10–50 nM) reduced cytochromesc and putativeaa3. Hypoxia (produced by superfusing with an medium equilibrated with 100% N2) reduced cytochromec but tended to oxidize the presumed cytochromeaa3. These effects were apparent at a medium pO2 of 100 torr or less. Fluorometry revealed reduction of NADH under both cyanide exposure and hypoxia. There was little or no interference by Hb when the preparations were carefully washed of remaining red cells. The precise site of the recorded mitochondria is unknown: they could have been located in the parenchymall cells (type I and/or II), nerve terminals, smooth muscle fibers, etc. Resolution of this point will need using dissociated carotid body cells. Further, the possible presence of a special respiratory pigment responding at a wavelength similar to that exciting cytochromeaa3 has not been discarded. Its study would require isolating carotid body mitochondria." @default.
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- W2019430912 title "Redox changes in the mouse carotid body during hypoxia" @default.
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- W2019430912 doi "https://doi.org/10.1016/0006-8993(85)90019-8" @default.
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