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- W2019433446 abstract "A gene encoding a β-mannanase (manA) has been cloned from an obligately anaerobic extreme thermophile, Caldicellulosiruptor strain Rt8B.4, which is most closely related to Caldicellulosiruptor saccharolyticus (formerly Caldocellum saccharolyticum). The gene codes for a multidomain enzyme with a C-terminal β-mannanase domain which was amplified by the polymerase chain reaction and cloned into a temperature-inducible expression vector in Escherichia coli. Sequence comparisons have shown that the Man domain of Rt8B.4 ManA is related to a thermophilic Dictyoglomus mannanase and a mesophilic mannanase from a Bacillus species. It appears to be unrelated to the β-mannanase domain of C. saccharolyticus, implying acquisition of the genes from unrelated sources by the two bacteria." @default.
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- W2019433446 date "1996-07-01" @default.
- W2019433446 modified "2023-09-27" @default.
- W2019433446 title "Sequencing, cloning and expression of a β-1,4-mannanase gene,manA, from the extremely thermophilic anaerobic bacterium,CaldicellulosiruptorRt8B.4" @default.
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- W2019433446 doi "https://doi.org/10.1111/j.1574-6968.1996.tb08360.x" @default.
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