FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019433804> ?p ?o ?g. }

• W2019433804 endingPage "90" @default.
• W2019433804 startingPage "81" @default.
• W2019433804 abstract "In recent years, using trigger-inducible mammalian gene switches to design sophisticated transcription-control networks has become standard practice in synthetic biology. These switches provide unprecedented precision, complexity and reliability when programming novel mammalian cell functions. Metabolite-responsive repressors of human-pathogenic bacteria are particularly attractive for use in these orthogonal synthetic mammalian gene switches because the trigger compound sensitivity often matches the human physiological range. We have designed both a bile acid-repressible (BEAROFF) as well as a bile-acid-inducible (BEARON) gene switch by capitalizing on components that have evolved to manage bile acid resistance in Campylobacter jejuni, the leading causative agent of human food-borne enteritis. We have shown that both of these switches enable bile acid-adjustable transgene expression in different mammalian cell lines as well as in mice. For the BEAROFF device, the C. jejuni repressor CmeR was fused to the VP16 transactivation domain to create a synthetic transactivator that activates minimal promoters containing tandem operator modules (Ocme) in a bile acid-repressible manner. Fusion of CmeR to a transsilencing domain resulted in an artificial transsilencer that binds and represses a constitutive Ocme-containing promoter until it is released by addition of bile acid (BEARON). A tailored multi-step tuning program for the inducible gene switch, which included the optimization of individual component performance, control of their relative abundances, the choice of the cell line and trigger compound, resulted in a BEARON device with significantly improved bile acid-responsive control characteristics. Synthetic metabolite-triggered gene switches that are able to interface with host metabolism may foster advances in future gene and cell-based therapies." @default.
• W2019433804 created "2016-06-24" @default.
• W2019433804 creator A5018310937 @default.
• W2019433804 creator A5061092011 @default.
• W2019433804 creator A5082252506 @default.
• W2019433804 date "2014-01-01" @default.
• W2019433804 modified "2023-09-26" @default.
• W2019433804 title "Bile acid-controlled transgene expression in mammalian cells and mice" @default.
• W2019433804 cites W1541662874 @default.
• W2019433804 cites W1645305843 @default.
• W2019433804 cites W1845425156 @default.
• W2019433804 cites W1966291152 @default.
• W2019433804 cites W1973890975 @default.
• W2019433804 cites W1978070564 @default.
• W2019433804 cites W1983730038 @default.
• W2019433804 cites W1984345984 @default.
• W2019433804 cites W1985590609 @default.
• W2019433804 cites W1988497176 @default.
• W2019433804 cites W1989024943 @default.
• W2019433804 cites W1997789388 @default.
• W2019433804 cites W2000232525 @default.
• W2019433804 cites W2001222172 @default.
• W2019433804 cites W2001679453 @default.
• W2019433804 cites W2006980110 @default.
• W2019433804 cites W2007769474 @default.
• W2019433804 cites W2011083779 @default.
• W2019433804 cites W2013234054 @default.
• W2019433804 cites W2016959279 @default.
• W2019433804 cites W2017741978 @default.
• W2019433804 cites W2021685870 @default.
• W2019433804 cites W2026267537 @default.
• W2019433804 cites W2026624424 @default.
• W2019433804 cites W2030193551 @default.
• W2019433804 cites W2030513350 @default.
• W2019433804 cites W2032123987 @default.
• W2019433804 cites W2035367704 @default.
• W2019433804 cites W2035640095 @default.
• W2019433804 cites W2036051969 @default.
• W2019433804 cites W2037735567 @default.
• W2019433804 cites W2040516865 @default.
• W2019433804 cites W2043711091 @default.
• W2019433804 cites W2050655839 @default.
• W2019433804 cites W2056686699 @default.
• W2019433804 cites W2057285600 @default.
• W2019433804 cites W2057994333 @default.
• W2019433804 cites W2063921800 @default.
• W2019433804 cites W2069382719 @default.
• W2019433804 cites W2071273298 @default.
• W2019433804 cites W2075792406 @default.
• W2019433804 cites W2076909664 @default.
• W2019433804 cites W2078810890 @default.
• W2019433804 cites W2079081483 @default.
• W2019433804 cites W2082263627 @default.
• W2019433804 cites W2082398508 @default.
• W2019433804 cites W2083344954 @default.
• W2019433804 cites W2086976827 @default.
• W2019433804 cites W2092059846 @default.
• W2019433804 cites W2093686699 @default.
• W2019433804 cites W2093906292 @default.
• W2019433804 cites W2100998682 @default.
• W2019433804 cites W2105014300 @default.
• W2019433804 cites W2117398483 @default.
• W2019433804 cites W2118540273 @default.
• W2019433804 cites W2121987905 @default.
• W2019433804 cites W2131184144 @default.
• W2019433804 cites W2132022155 @default.
• W2019433804 cites W2134759932 @default.
• W2019433804 cites W2134809564 @default.
• W2019433804 cites W2138520317 @default.
• W2019433804 cites W2145668963 @default.
• W2019433804 cites W2158609797 @default.
• W2019433804 cites W2162584238 @default.
• W2019433804 cites W2167456385 @default.
• W2019433804 cites W2167652197 @default.
• W2019433804 cites W2170469583 @default.
• W2019433804 doi "https://doi.org/10.1016/j.ymben.2013.11.003" @default.
• W2019433804 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24280297" @default.
• W2019433804 hasPublicationYear "2014" @default.
• W2019433804 type Work @default.
• W2019433804 sameAs 2019433804 @default.
• W2019433804 citedByCount "20" @default.
• W2019433804 countsByYear W20194338042013 @default.
• W2019433804 countsByYear W20194338042014 @default.
• W2019433804 countsByYear W20194338042015 @default.
• W2019433804 countsByYear W20194338042016 @default.
• W2019433804 countsByYear W20194338042017 @default.
• W2019433804 countsByYear W20194338042018 @default.
• W2019433804 countsByYear W20194338042020 @default.
• W2019433804 countsByYear W20194338042021 @default.
• W2019433804 countsByYear W20194338042022 @default.
• W2019433804 crossrefType "journal-article" @default.
• W2019433804 hasAuthorship W2019433804A5018310937 @default.
• W2019433804 hasAuthorship W2019433804A5061092011 @default.
• W2019433804 hasAuthorship W2019433804A5082252506 @default.
• W2019433804 hasConcept C102230213 @default.
• W2019433804 hasConcept C104317684 @default.
• W2019433804 hasConcept C1292079 @default.
• W2019433804 hasConcept C150194340 @default.

• next