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- W2019437629 abstract "Regulation of DNA synthesis was investigated in SV40 transformed 3T3 cells exhibiting variable growth rates and residence times in S phase when cultured in the presence of different serum concentrations. Pulse-labeled DNA was chased into large molecular weight material in vivo much more slowly in slowly growing cells than in cells growing at the normal rate. Consistent with this, the joining of short (<10 S) chains to form long (>10 S) chains by whole cell lysate system in vitro was greatly impaired in slowly growing cells compared to controls. Thus the lengthening of S phase in SV3T3 cells growing slowly in low serum is reflected in a reduced rate of DNA chain elongation. The presence of cycloheximide during chase in vivo reduced the rate of conversion of pulse-labeled molecules into large molecular weight DNA in both slowly growing and normally growing cells." @default.
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- W2019437629 date "1977-10-01" @default.
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- W2019437629 title "Regulation of DNA chain elongation in SV3T3 cells in relation to growth rate" @default.
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- W2019437629 doi "https://doi.org/10.1016/0005-2787(77)90100-9" @default.
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