FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019466574> ?p ?o ?g. }

• W2019466574 endingPage "496" @default.
• W2019466574 startingPage "489" @default.
• W2019466574 abstract "Phenotypic heterogeneity in bacterial populations is thought to contribute to a number of important phenomena including sporulation and persistence. The latter has clinical implications in many diseases such as tuberculosis, where persistence of Mycobacterium tuberculosis within the human host is believed to be the root cause of latent tuberculosis and the ability of a minority population of cells to survive antibiotic exposure, despite being genetically identical to the bulk population that are killed. However, phenotypic variations caused by non-genetic mechanisms are difficult to study because of the transient nature of the persistent state and thereby the requirement to observe individual cells in real-time. Recently, microfluidics, combined with time-lapse microscopy, has become a powerful tool for studying population heterogeneity in bacteria. However, growth and replication of mycobacterial cells provide particular problems for the development of microfluidic systems due to their tendency to grow in three dimensions. We here describe a novel microfluidic device for the observation of growth and antibiotic killing in individual mycobacterial cells. We constructed a microfluidic device suitable for studying single cell behavior in mycobacteria. The growth of single cells of Mycobacterium smegmatis expressing green fluorescent protein was monitored using a confocal laser scanning microscope. Within the device M. smegmatis cells were tightly confined within a hydrogel matrix thus promoting planar growth. Cell growth and killing was observed in the device with dead cells highlighted by uptake of propidium iodide. Conclusions/Significance. We demonstrate that our device allows real-time analysis and long-term culture of single cells of mycobacteria, and is able to support the study of cell death during the application of antibiotics. The device will allow observation of individual cells' cell genealogy to be determined and direct observation of rare states, such as persistence." @default.
• W2019466574 created "2016-06-24" @default.
• W2019466574 creator A5021507960 @default.
• W2019466574 creator A5028497882 @default.
• W2019466574 creator A5035052784 @default.
• W2019466574 creator A5083104489 @default.
• W2019466574 date "2012-11-01" @default.
• W2019466574 modified "2023-09-24" @default.
• W2019466574 title "A microfluidic system for long-term time-lapse microscopy studies of mycobacteria" @default.
• W2019466574 cites W1575619134 @default.
• W2019466574 cites W1767889474 @default.
• W2019466574 cites W1969244937 @default.
• W2019466574 cites W1969782428 @default.
• W2019466574 cites W1970853823 @default.
• W2019466574 cites W1976256007 @default.
• W2019466574 cites W1988791304 @default.
• W2019466574 cites W1996666978 @default.
• W2019466574 cites W1997057704 @default.
• W2019466574 cites W2005782605 @default.
• W2019466574 cites W2009253350 @default.
• W2019466574 cites W2019835759 @default.
• W2019466574 cites W2026322027 @default.
• W2019466574 cites W2029051828 @default.
• W2019466574 cites W2033117797 @default.
• W2019466574 cites W2034374036 @default.
• W2019466574 cites W2044355789 @default.
• W2019466574 cites W2045825103 @default.
• W2019466574 cites W2049522952 @default.
• W2019466574 cites W2078782272 @default.
• W2019466574 cites W2082573007 @default.
• W2019466574 cites W2094517427 @default.
• W2019466574 cites W2094565857 @default.
• W2019466574 cites W2109431702 @default.
• W2019466574 cites W2110760619 @default.
• W2019466574 cites W2114870653 @default.
• W2019466574 cites W2125112523 @default.
• W2019466574 cites W2126733261 @default.
• W2019466574 cites W2131970139 @default.
• W2019466574 cites W2139387392 @default.
• W2019466574 cites W2148323109 @default.
• W2019466574 cites W2155180530 @default.
• W2019466574 cites W2160479796 @default.
• W2019466574 cites W2167373328 @default.
• W2019466574 cites W2167588734 @default.
• W2019466574 doi "https://doi.org/10.1016/j.tube.2012.06.006" @default.
• W2019466574 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22954584" @default.
• W2019466574 hasPublicationYear "2012" @default.
• W2019466574 type Work @default.
• W2019466574 sameAs 2019466574 @default.
• W2019466574 citedByCount "40" @default.
• W2019466574 countsByYear W20194665742013 @default.
• W2019466574 countsByYear W20194665742014 @default.
• W2019466574 countsByYear W20194665742015 @default.
• W2019466574 countsByYear W20194665742016 @default.
• W2019466574 countsByYear W20194665742017 @default.
• W2019466574 countsByYear W20194665742018 @default.
• W2019466574 countsByYear W20194665742019 @default.
• W2019466574 countsByYear W20194665742020 @default.
• W2019466574 countsByYear W20194665742021 @default.
• W2019466574 countsByYear W20194665742022 @default.
• W2019466574 countsByYear W20194665742023 @default.
• W2019466574 crossrefType "journal-article" @default.
• W2019466574 hasAuthorship W2019466574A5021507960 @default.
• W2019466574 hasAuthorship W2019466574A5028497882 @default.
• W2019466574 hasAuthorship W2019466574A5035052784 @default.
• W2019466574 hasAuthorship W2019466574A5083104489 @default.
• W2019466574 hasConcept C142724271 @default.
• W2019466574 hasConcept C171250308 @default.
• W2019466574 hasConcept C190283241 @default.
• W2019466574 hasConcept C192562407 @default.
• W2019466574 hasConcept C2775934118 @default.
• W2019466574 hasConcept C2777204207 @default.
• W2019466574 hasConcept C2777975735 @default.
• W2019466574 hasConcept C2780374374 @default.
• W2019466574 hasConcept C2781069245 @default.
• W2019466574 hasConcept C2908647359 @default.
• W2019466574 hasConcept C31573885 @default.
• W2019466574 hasConcept C523546767 @default.
• W2019466574 hasConcept C54355233 @default.
• W2019466574 hasConcept C58123911 @default.
• W2019466574 hasConcept C71924100 @default.
• W2019466574 hasConcept C8673954 @default.
• W2019466574 hasConcept C86803240 @default.
• W2019466574 hasConcept C89423630 @default.
• W2019466574 hasConcept C95444343 @default.
• W2019466574 hasConcept C99454951 @default.
• W2019466574 hasConceptScore W2019466574C142724271 @default.
• W2019466574 hasConceptScore W2019466574C171250308 @default.
• W2019466574 hasConceptScore W2019466574C190283241 @default.
• W2019466574 hasConceptScore W2019466574C192562407 @default.
• W2019466574 hasConceptScore W2019466574C2775934118 @default.
• W2019466574 hasConceptScore W2019466574C2777204207 @default.
• W2019466574 hasConceptScore W2019466574C2777975735 @default.
• W2019466574 hasConceptScore W2019466574C2780374374 @default.
• W2019466574 hasConceptScore W2019466574C2781069245 @default.
• W2019466574 hasConceptScore W2019466574C2908647359 @default.
• W2019466574 hasConceptScore W2019466574C31573885 @default.
• W2019466574 hasConceptScore W2019466574C523546767 @default.

• next