FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019468093> ?p ?o ?g. }

• W2019468093 endingPage "223" @default.
• W2019468093 startingPage "219" @default.
• W2019468093 abstract "Objective. – The feasibility of gene transfer to myocardial tissue using viral vectors was investigated over the last few years. In this study we report gene transfer using a recently described improved of Herpes simplex virus (HSV-1)-derived amplicon vectors and demonstrate that these vectors are a powerful and potentially very interesting tool for gene transfer into neonatal primary as well as in adult cardiac myocytes. Methods and results. – Non-pathogenic HSV-1 amplicon vectors simultaneously expressing GFP and LacZ were constructed using a novel helper system that yields essentially helper-free vector particles. These vectors were used to infect either cultured primary neonatal rat cardiomyocytes or adult cardiac tissue. Transgenic expression was quantified using a FACS (GFP) or X-gal staining (LacZ). Infection of primary cardiomyocytes showed efficient transduction even at very low multiplicity of infection (MOI), and expression increased with the infectious dose. By investigating release of lactate dehydrogenase (LDH) or spontaneous beating of the cells, we failed to detect cytotoxic effects in cardiomyocytes infected at high MOI. Thin slices of adult cardiac tissue placed in medium containing vectors also showed very good levels of transduction, without any evidence of toxic effects. Conclusions. – Helper-free amplicon vectors very efficiently transduce genes into cardiomyocytes. Our results indicate similar or better transduction efficiencies than those reported using other vector systems. Furthermore, the very high transgenic capacity of amplicon vectors (up to 150 kbp) makes these vectors a unique and very suitable system to transduce large genomic sequences into cardiomyocytes." @default.
• W2019468093 created "2016-06-24" @default.
• W2019468093 creator A5009895142 @default.
• W2019468093 creator A5028721928 @default.
• W2019468093 creator A5035929683 @default.
• W2019468093 creator A5038398664 @default.
• W2019468093 creator A5042601884 @default.
• W2019468093 creator A5081220247 @default.
• W2019468093 date "2005-01-01" @default.
• W2019468093 modified "2023-09-29" @default.
• W2019468093 title "Efficient and non-toxic gene transfer to cardiomyocytes using novel generation amplicon vectors derived from HSV-1" @default.
• W2019468093 cites W1490731141 @default.
• W2019468093 cites W1964319168 @default.
• W2019468093 cites W1981369720 @default.
• W2019468093 cites W1988384128 @default.
• W2019468093 cites W1991037396 @default.
• W2019468093 cites W1991711092 @default.
• W2019468093 cites W1996916131 @default.
• W2019468093 cites W1997426616 @default.
• W2019468093 cites W2031102459 @default.
• W2019468093 cites W2066236835 @default.
• W2019468093 cites W2087791213 @default.
• W2019468093 cites W2130522867 @default.
• W2019468093 cites W2146617313 @default.
• W2019468093 cites W2154736819 @default.
• W2019468093 cites W2174467695 @default.
• W2019468093 cites W2177597220 @default.
• W2019468093 cites W2257474809 @default.
• W2019468093 doi "https://doi.org/10.1016/j.yjmcc.2004.11.014" @default.
• W2019468093 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15623439" @default.
• W2019468093 hasPublicationYear "2005" @default.
• W2019468093 type Work @default.
• W2019468093 sameAs 2019468093 @default.
• W2019468093 citedByCount "15" @default.
• W2019468093 countsByYear W20194680932012 @default.
• W2019468093 countsByYear W20194680932013 @default.
• W2019468093 countsByYear W20194680932014 @default.
• W2019468093 countsByYear W20194680932017 @default.
• W2019468093 countsByYear W20194680932019 @default.
• W2019468093 countsByYear W20194680932023 @default.
• W2019468093 crossrefType "journal-article" @default.
• W2019468093 hasAuthorship W2019468093A5009895142 @default.
• W2019468093 hasAuthorship W2019468093A5028721928 @default.
• W2019468093 hasAuthorship W2019468093A5035929683 @default.
• W2019468093 hasAuthorship W2019468093A5038398664 @default.
• W2019468093 hasAuthorship W2019468093A5042601884 @default.
• W2019468093 hasAuthorship W2019468093A5081220247 @default.
• W2019468093 hasConcept C102230213 @default.
• W2019468093 hasConcept C104317684 @default.
• W2019468093 hasConcept C111599444 @default.
• W2019468093 hasConcept C142613039 @default.
• W2019468093 hasConcept C15152581 @default.
• W2019468093 hasConcept C153911025 @default.
• W2019468093 hasConcept C159047783 @default.
• W2019468093 hasConcept C203233990 @default.
• W2019468093 hasConcept C207200792 @default.
• W2019468093 hasConcept C2522874641 @default.
• W2019468093 hasConcept C2781196997 @default.
• W2019468093 hasConcept C32470452 @default.
• W2019468093 hasConcept C40767141 @default.
• W2019468093 hasConcept C49105822 @default.
• W2019468093 hasConcept C54355233 @default.
• W2019468093 hasConcept C55493867 @default.
• W2019468093 hasConcept C8185291 @default.
• W2019468093 hasConcept C86803240 @default.
• W2019468093 hasConcept C92087593 @default.
• W2019468093 hasConcept C95444343 @default.
• W2019468093 hasConceptScore W2019468093C102230213 @default.
• W2019468093 hasConceptScore W2019468093C104317684 @default.
• W2019468093 hasConceptScore W2019468093C111599444 @default.
• W2019468093 hasConceptScore W2019468093C142613039 @default.
• W2019468093 hasConceptScore W2019468093C15152581 @default.
• W2019468093 hasConceptScore W2019468093C153911025 @default.
• W2019468093 hasConceptScore W2019468093C159047783 @default.
• W2019468093 hasConceptScore W2019468093C203233990 @default.
• W2019468093 hasConceptScore W2019468093C207200792 @default.
• W2019468093 hasConceptScore W2019468093C2522874641 @default.
• W2019468093 hasConceptScore W2019468093C2781196997 @default.
• W2019468093 hasConceptScore W2019468093C32470452 @default.
• W2019468093 hasConceptScore W2019468093C40767141 @default.
• W2019468093 hasConceptScore W2019468093C49105822 @default.
• W2019468093 hasConceptScore W2019468093C54355233 @default.
• W2019468093 hasConceptScore W2019468093C55493867 @default.
• W2019468093 hasConceptScore W2019468093C8185291 @default.
• W2019468093 hasConceptScore W2019468093C86803240 @default.
• W2019468093 hasConceptScore W2019468093C92087593 @default.
• W2019468093 hasConceptScore W2019468093C95444343 @default.
• W2019468093 hasIssue "1" @default.
• W2019468093 hasLocation W20194680931 @default.
• W2019468093 hasLocation W20194680932 @default.
• W2019468093 hasLocation W20194680933 @default.
• W2019468093 hasOpenAccess W2019468093 @default.
• W2019468093 hasPrimaryLocation W20194680931 @default.
• W2019468093 hasRelatedWork W1990941594 @default.
• W2019468093 hasRelatedWork W1992792239 @default.
• W2019468093 hasRelatedWork W2007276010 @default.
• W2019468093 hasRelatedWork W2062787494 @default.
• W2019468093 hasRelatedWork W2167631030 @default.

• next