Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019499119> ?p ?o ?g. }
- W2019499119 endingPage "21" @default.
- W2019499119 startingPage "15" @default.
- W2019499119 abstract "Spontaneous polyploids and mosaics have often been observed in artificially propagated larvae of the Japanese eel, Anguilla japonica. However, the mechanisms responsible for such unusual cytotypes are unclear. In this study, we examined the relationship of such polyploidization and mosaicisms in larvae resulting from artificial propagation to egg quality (fertilization rate and hatching rate) and viability of larvae, and then clarified the inducing factors and the mechanism for occurrence of such phenomena. Eggs stripped from females after induced maturation were artificially inseminated with sperm pre-cultured with artificial seminal plasma. Ploidy was determined by measuring the relative DNA content of the nuclei with flow cytometry. Of 968 embryos from 32 full-sib families, 9.1% were determined to be abnormal, most of which were triploids (86.5% of abnormal embryos); others were haploids (1.1%), aneuploids (2.3%), and mosaics (10.1%). The percentage of normal diploids from each family varied between 56.3% and 100% (90.9 ± 11.7%, n = 32). A significant positive correlation was found between the fertilization rate (P < 0.001) or the hatching rate (P < 0.001) and the percentage of diploids. Survival rate of triploid eels was similar to diploid eels at 10 days after fertilization whereas aneuploids were inviable. When eggs were left in the body cavity of the female for four hours after ovulation and subsequently fertilized, the percentage of diploids decreased. We tried to elucidate the cause for the occurrence of spontaneous triploids by genetic analysis using 26 microsatellite DNA markers, which have been developed and mapped in relation to the centromere. These results suggest that the occurrence of cytogenetically unusual progeny is associated with over-ripening or aging of ova caused by the lapse of time from ovulation until fertilization, and spontaneous triploid larvae are derived from the duplication of the maternal chromosome set by inhibition of the second polar body release after normal meiosis I (crossing over) in oocyte and fertilization with normal sperm." @default.
- W2019499119 created "2016-06-24" @default.
- W2019499119 creator A5020204829 @default.
- W2019499119 creator A5022607246 @default.
- W2019499119 creator A5030671868 @default.
- W2019499119 creator A5031017036 @default.
- W2019499119 creator A5031493092 @default.
- W2019499119 creator A5051457120 @default.
- W2019499119 creator A5069465380 @default.
- W2019499119 date "2013-08-01" @default.
- W2019499119 modified "2023-09-26" @default.
- W2019499119 title "Post-ovulatory oocyte aging induces spontaneous occurrence of polyploids and mosaics in artificial fertilization of Japanese eel, Anguilla japonica" @default.
- W2019499119 cites W1518673844 @default.
- W2019499119 cites W1553131230 @default.
- W2019499119 cites W1595483961 @default.
- W2019499119 cites W1755584256 @default.
- W2019499119 cites W1895043251 @default.
- W2019499119 cites W1977618351 @default.
- W2019499119 cites W1986286475 @default.
- W2019499119 cites W2012102507 @default.
- W2019499119 cites W2015433203 @default.
- W2019499119 cites W2016355884 @default.
- W2019499119 cites W2026270741 @default.
- W2019499119 cites W2029677297 @default.
- W2019499119 cites W2053762690 @default.
- W2019499119 cites W2057240799 @default.
- W2019499119 cites W2060997764 @default.
- W2019499119 cites W2061239456 @default.
- W2019499119 cites W2062467266 @default.
- W2019499119 cites W2065944198 @default.
- W2019499119 cites W2070470556 @default.
- W2019499119 cites W2073583094 @default.
- W2019499119 cites W2085976841 @default.
- W2019499119 cites W2129606528 @default.
- W2019499119 cites W2202372932 @default.
- W2019499119 cites W2317943701 @default.
- W2019499119 cites W398391364 @default.
- W2019499119 doi "https://doi.org/10.1016/j.aquaculture.2013.04.016" @default.
- W2019499119 hasPublicationYear "2013" @default.
- W2019499119 type Work @default.
- W2019499119 sameAs 2019499119 @default.
- W2019499119 citedByCount "20" @default.
- W2019499119 countsByYear W20194991192013 @default.
- W2019499119 countsByYear W20194991192015 @default.
- W2019499119 countsByYear W20194991192016 @default.
- W2019499119 countsByYear W20194991192017 @default.
- W2019499119 countsByYear W20194991192018 @default.
- W2019499119 countsByYear W20194991192019 @default.
- W2019499119 countsByYear W20194991192020 @default.
- W2019499119 countsByYear W20194991192021 @default.
- W2019499119 countsByYear W20194991192023 @default.
- W2019499119 crossrefType "journal-article" @default.
- W2019499119 hasAuthorship W2019499119A5020204829 @default.
- W2019499119 hasAuthorship W2019499119A5022607246 @default.
- W2019499119 hasAuthorship W2019499119A5030671868 @default.
- W2019499119 hasAuthorship W2019499119A5031017036 @default.
- W2019499119 hasAuthorship W2019499119A5031493092 @default.
- W2019499119 hasAuthorship W2019499119A5051457120 @default.
- W2019499119 hasAuthorship W2019499119A5069465380 @default.
- W2019499119 hasBestOaLocation W20194991191 @default.
- W2019499119 hasConcept C104317684 @default.
- W2019499119 hasConcept C105702510 @default.
- W2019499119 hasConcept C140793950 @default.
- W2019499119 hasConcept C143191323 @default.
- W2019499119 hasConcept C16685009 @default.
- W2019499119 hasConcept C173758957 @default.
- W2019499119 hasConcept C196843134 @default.
- W2019499119 hasConcept C2776690073 @default.
- W2019499119 hasConcept C2777292126 @default.
- W2019499119 hasConcept C2777702977 @default.
- W2019499119 hasConcept C2777744765 @default.
- W2019499119 hasConcept C2779234561 @default.
- W2019499119 hasConcept C2779409272 @default.
- W2019499119 hasConcept C2781087480 @default.
- W2019499119 hasConcept C2910203288 @default.
- W2019499119 hasConcept C54355233 @default.
- W2019499119 hasConcept C59822182 @default.
- W2019499119 hasConcept C71924100 @default.
- W2019499119 hasConcept C86803240 @default.
- W2019499119 hasConcept C88972607 @default.
- W2019499119 hasConcept C90856448 @default.
- W2019499119 hasConceptScore W2019499119C104317684 @default.
- W2019499119 hasConceptScore W2019499119C105702510 @default.
- W2019499119 hasConceptScore W2019499119C140793950 @default.
- W2019499119 hasConceptScore W2019499119C143191323 @default.
- W2019499119 hasConceptScore W2019499119C16685009 @default.
- W2019499119 hasConceptScore W2019499119C173758957 @default.
- W2019499119 hasConceptScore W2019499119C196843134 @default.
- W2019499119 hasConceptScore W2019499119C2776690073 @default.
- W2019499119 hasConceptScore W2019499119C2777292126 @default.
- W2019499119 hasConceptScore W2019499119C2777702977 @default.
- W2019499119 hasConceptScore W2019499119C2777744765 @default.
- W2019499119 hasConceptScore W2019499119C2779234561 @default.
- W2019499119 hasConceptScore W2019499119C2779409272 @default.
- W2019499119 hasConceptScore W2019499119C2781087480 @default.
- W2019499119 hasConceptScore W2019499119C2910203288 @default.
- W2019499119 hasConceptScore W2019499119C54355233 @default.
- W2019499119 hasConceptScore W2019499119C59822182 @default.