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- W2019524674 abstract "Point mutation in the nucleotide sequence of the structural genes for the TEM-type penicillinases can broaden their substrate spectrum towards all beta-lactams except cephamicins and imipenem. We describe here hybridization techniques for the detection of point mutations by non-radioactive oligonucleotide probes with plasmid DNA carrying bla T genes immobilized in polystyrene microwells. After hybridization in discriminating conditions with corresponding biotinylated oligonucleotide probes, the hybrids were detected by using a streptavidin-alkaline phosphatase conjugate and a fluorogenic substrate, 4-methylumbelliferyl-phosphate. The adsorption of DNA to microwells used in the present work was found to be independent of Mg2+ and Na+ concentrations. By this method, less than 3 fmols of target DNA were sufficient for the detection of point mutation." @default.
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- W2019524674 date "1992-02-01" @default.
- W2019524674 modified "2023-09-23" @default.
- W2019524674 title "Detection of point mutation in bla T genes of enterobacteriaceae by biotinylated oligonucleotide probes using microwell hybridization and enzymofluorometric method" @default.
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- W2019524674 doi "https://doi.org/10.1016/0890-8508(92)90074-8" @default.
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