FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019587003> ?p ?o ?g. }

• W2019587003 endingPage "59" @default.
• W2019587003 startingPage "51" @default.
• W2019587003 abstract "Mechanosensitive channels are present in almost every living cell, yet the evidence for their functional presence in T lymphocytes is absent. In this study, by means of the patch-clamp technique in attached and inside-out modes, we have characterized cationic channels, rapidly activated by membrane stretch in Jurkat T lymphoblasts. The half-activation was achieved at a negative pressure of ~ 50 mm Hg. In attached mode, single channel currents displayed an inward rectification and the unitary conductance of ~ 40 pS at zero command voltage. In excised inside-out patches the rectification was transformed to an outward one. Mechanosensitive channels weakly discriminated between mono- and divalent cations (PCa/PNa ~ 1) and were equally permeable for Ca2 + and Mg2 +. Pharmacological analysis showed that the mechanosensitive channels were potently blocked by amiloride (1 mM) and Gd3 + (10 μM) in a voltage-dependent manner. They were also almost completely blocked by ruthenium red (1 μM) and SKF 96365 (250 μM), inhibitors of transient receptor potential vanilloid 2 (TRPV2) channels. At the same time, the channels were insensitive to 2-aminoethoxydiphenyl borate (2-APB, 100 μM) or N-(p-amylcinnamoyl)anthranilic acid (ACA, 50 μM), antagonists of transient receptor potential canonical (TRPC) or transient receptor potential melastatin (TRPM) channels, respectively. Human TRPV2 siRNA virtually abolished the stretch-activated current. TRPV2 are channels with multifaceted functions and regulatory mechanisms, with potentially important roles in the lymphocyte Ca2 + signaling. Implications of their regulation by mechanical stress are discussed in the context of lymphoid cells functions." @default.
• W2019587003 created "2016-06-24" @default.
• W2019587003 creator A5008914764 @default.
• W2019587003 creator A5020368412 @default.
• W2019587003 creator A5033322392 @default.
• W2019587003 creator A5061714297 @default.
• W2019587003 creator A5088801175 @default.
• W2019587003 creator A5090020641 @default.
• W2019587003 date "2015-01-01" @default.
• W2019587003 modified "2023-09-27" @default.
• W2019587003 title "Mechanosensitive Ca2+-permeable channels in human leukemic cells: Pharmacological and molecular evidence for TRPV2" @default.
• W2019587003 cites W1495409864 @default.
• W2019587003 cites W1513561540 @default.
• W2019587003 cites W1542697059 @default.
• W2019587003 cites W1554059507 @default.
• W2019587003 cites W1567081747 @default.
• W2019587003 cites W1596758664 @default.
• W2019587003 cites W1840415699 @default.
• W2019587003 cites W1964159573 @default.
• W2019587003 cites W1967170563 @default.
• W2019587003 cites W1970629236 @default.
• W2019587003 cites W1975461211 @default.
• W2019587003 cites W1976176467 @default.
• W2019587003 cites W1980798939 @default.
• W2019587003 cites W1985474963 @default.
• W2019587003 cites W1985948706 @default.
• W2019587003 cites W1991972386 @default.
• W2019587003 cites W1998329479 @default.
• W2019587003 cites W1999720993 @default.
• W2019587003 cites W2000874747 @default.
• W2019587003 cites W2001453486 @default.
• W2019587003 cites W2003380041 @default.
• W2019587003 cites W2003403297 @default.
• W2019587003 cites W2006033801 @default.
• W2019587003 cites W2006149052 @default.
• W2019587003 cites W2007661838 @default.
• W2019587003 cites W2013962899 @default.
• W2019587003 cites W2017253185 @default.
• W2019587003 cites W2019879792 @default.
• W2019587003 cites W2021429824 @default.
• W2019587003 cites W2021569813 @default.
• W2019587003 cites W2026702641 @default.
• W2019587003 cites W2030790147 @default.
• W2019587003 cites W2032688072 @default.
• W2019587003 cites W2039493692 @default.
• W2019587003 cites W2042673902 @default.
• W2019587003 cites W2042968117 @default.
• W2019587003 cites W2044103292 @default.
• W2019587003 cites W2048347960 @default.
• W2019587003 cites W2051511150 @default.
• W2019587003 cites W2063639334 @default.
• W2019587003 cites W2068912108 @default.
• W2019587003 cites W2071763701 @default.
• W2019587003 cites W2083206813 @default.
• W2019587003 cites W2086405553 @default.
• W2019587003 cites W2101233587 @default.
• W2019587003 cites W2101626632 @default.
• W2019587003 cites W2104587256 @default.
• W2019587003 cites W2109466522 @default.
• W2019587003 cites W2114570899 @default.
• W2019587003 cites W2123673561 @default.
• W2019587003 cites W2126283502 @default.
• W2019587003 cites W2136018765 @default.
• W2019587003 cites W2137290540 @default.
• W2019587003 cites W2138841389 @default.
• W2019587003 cites W2139149736 @default.
• W2019587003 cites W2141600581 @default.
• W2019587003 cites W2146817455 @default.
• W2019587003 cites W2147783554 @default.
• W2019587003 cites W2148761780 @default.
• W2019587003 cites W2149429155 @default.
• W2019587003 cites W2152481050 @default.
• W2019587003 cites W2153704362 @default.
• W2019587003 cites W2155469071 @default.
• W2019587003 cites W2164157263 @default.
• W2019587003 cites W2164683872 @default.
• W2019587003 cites W2166046655 @default.
• W2019587003 cites W2168934695 @default.
• W2019587003 cites W2171315885 @default.
• W2019587003 cites W2186295141 @default.
• W2019587003 cites W2320569584 @default.
• W2019587003 cites W4248092016 @default.
• W2019587003 doi "https://doi.org/10.1016/j.bbamem.2014.09.008" @default.
• W2019587003 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25268680" @default.
• W2019587003 hasPublicationYear "2015" @default.
• W2019587003 type Work @default.
• W2019587003 sameAs 2019587003 @default.
• W2019587003 citedByCount "37" @default.
• W2019587003 countsByYear W20195870032015 @default.
• W2019587003 countsByYear W20195870032016 @default.
• W2019587003 countsByYear W20195870032017 @default.
• W2019587003 countsByYear W20195870032018 @default.
• W2019587003 countsByYear W20195870032019 @default.
• W2019587003 countsByYear W20195870032020 @default.
• W2019587003 countsByYear W20195870032021 @default.
• W2019587003 countsByYear W20195870032022 @default.
• W2019587003 countsByYear W20195870032023 @default.
• W2019587003 crossrefType "journal-article" @default.

• next