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• W2019592010 abstract "From 1999 to 2002, 246 serum samples taken from polytransfused children were tested for the presence of GB virus C (GBV-C) RNA using a real-time reverse transcription-polymerase chain reaction (RT-PCR) assay. This assay was based on the TaqMan technology and allowed viral load determination in infected children with a dynamic range from 103 to 107 genome equivalent (gEq) copies/ml. The limit of detection was estimated to 619 gEq copies/ml with a ≥95% probability of a positive result. Thirty five sera were found to be GBV-C RNA positive, corresponding to a prevalence of GBV-C of 14.2%. The mean viral load was high, i.e., 6 ± 1.4 log (range 3.22–7.42) gEq copies/ml, but low viral loads were also detected. Sequencing of the 5′-untranslated region (UTR) identified a majority of genotype 2 strains (82%) distributed into two subtypes, 88.5% genotype 2a and 11.5% genotype 2b. In conclusion, GBV-C active infection is very frequent in exposed populations such as polytransfused children. GBV-C RNA quantitation using real-time assay may be useful for diagnosis and follow-up of the natural history of GBV-C infection. J. Med. Virol. 73:596–600, 2004. © 2004 Wiley-Liss, Inc." @default.
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• W2019592010 date "2004-01-01" @default.
• W2019592010 modified "2023-10-01" @default.
• W2019592010 title "Epidemiological and quantitative study of GBV-C infection in french polytransfused children" @default.
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• W2019592010 doi "https://doi.org/10.1002/jmv.20131" @default.
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