FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019607276> ?p ?o ?g. }

• W2019607276 endingPage "176" @default.
• W2019607276 startingPage "167" @default.
• W2019607276 abstract "The present study examines the synergistic activation of PKC by arachidonic acid and diacylglycerols in phospholipid vesicles and demonstrates that this combination of activators leads to the formation of a constitutively active, phospholipid-bound form of the enzyme. Activation of PKC was almost entirely calcium-dependent with vesicles containing dioleoylglycerol alone. In contrast, considerable calcium-independent activity was observed when vesicles contained both a diacylglycerol and free arachidonic acid. High-affinity association of enzyme activity with diacylglycerol-containing vesicles was calcium dependent and reversible. However, addition of arachidonic acid to diacylglycerol-containing vesicles resulted in irreversible PKC binding in the absence of calcium. Immunoblot analysis indicated that the calcium-independent binding was not isozyme-specific. The activity of the vesicle-associated PKC, bound to vesicles in the absence of calcium, was predominantly calcium-dependent. On the other hand, when the binding and isolation of vesicle-bound enzyme was conducted in the presence of calcium, the subsequent activity was almost entirely resistant to calcium chelation. This vesicle-associated form of the enzyme, when detergent extracted and recombined with phospholipid vesicles, maintained significant ‘constitutive’ activity (activity in the absence of both diacylglycerol and calcium). The data from this in vitro system provide the basis for a model of the physiological regulation of PKC in which the combined actions of arachidonate and diacylglycerol facilitate the stable formation of a tightly membrane-associated, intrinsically active form of PKC." @default.
• W2019607276 created "2016-06-24" @default.
• W2019607276 creator A5005882758 @default.
• W2019607276 creator A5072355639 @default.
• W2019607276 creator A5074933659 @default.
• W2019607276 date "1996-10-01" @default.
• W2019607276 modified "2023-10-14" @default.
• W2019607276 title "Synergistic activation of protein kinase C by arachidonic acid and diacylglycerols in vitro: generation of a stable membrane-bound, cofactor-independent state of protein kinase C activity" @default.
• W2019607276 cites W1426584128 @default.
• W2019607276 cites W1483045044 @default.
• W2019607276 cites W1483867615 @default.
• W2019607276 cites W1502793076 @default.
• W2019607276 cites W1503469366 @default.
• W2019607276 cites W1521493025 @default.
• W2019607276 cites W1522765298 @default.
• W2019607276 cites W1545100002 @default.
• W2019607276 cites W1553713007 @default.
• W2019607276 cites W1569108584 @default.
• W2019607276 cites W1570470675 @default.
• W2019607276 cites W1596133917 @default.
• W2019607276 cites W1603607026 @default.
• W2019607276 cites W1895065514 @default.
• W2019607276 cites W1970991463 @default.
• W2019607276 cites W1975445973 @default.
• W2019607276 cites W1977672499 @default.
• W2019607276 cites W1977777746 @default.
• W2019607276 cites W1980039319 @default.
• W2019607276 cites W1982687189 @default.
• W2019607276 cites W1985905523 @default.
• W2019607276 cites W1987212870 @default.
• W2019607276 cites W1993602573 @default.
• W2019607276 cites W1997535255 @default.
• W2019607276 cites W2002984440 @default.
• W2019607276 cites W2005291650 @default.
• W2019607276 cites W2005516514 @default.
• W2019607276 cites W2016477781 @default.
• W2019607276 cites W2019464191 @default.
• W2019607276 cites W2020220207 @default.
• W2019607276 cites W2023447656 @default.
• W2019607276 cites W2027126024 @default.
• W2019607276 cites W2027673151 @default.
• W2019607276 cites W2032993257 @default.
• W2019607276 cites W2037238984 @default.
• W2019607276 cites W2037512229 @default.
• W2019607276 cites W2039542771 @default.
• W2019607276 cites W2041561625 @default.
• W2019607276 cites W2044851240 @default.
• W2019607276 cites W2059729679 @default.
• W2019607276 cites W2062071134 @default.
• W2019607276 cites W2068270309 @default.
• W2019607276 cites W2071272951 @default.
• W2019607276 cites W2071643523 @default.
• W2019607276 cites W2083432361 @default.
• W2019607276 cites W2083611460 @default.
• W2019607276 cites W2088103235 @default.
• W2019607276 cites W2094434639 @default.
• W2019607276 cites W2117227310 @default.
• W2019607276 cites W2126876185 @default.
• W2019607276 cites W2153046617 @default.
• W2019607276 cites W2410660408 @default.
• W2019607276 cites W953349002 @default.
• W2019607276 doi "https://doi.org/10.1016/0304-4165(96)00063-3" @default.
• W2019607276 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8898879" @default.
• W2019607276 hasPublicationYear "1996" @default.
• W2019607276 type Work @default.
• W2019607276 sameAs 2019607276 @default.
• W2019607276 citedByCount "38" @default.
• W2019607276 countsByYear W20196072762013 @default.
• W2019607276 countsByYear W20196072762015 @default.
• W2019607276 countsByYear W20196072762021 @default.
• W2019607276 crossrefType "journal-article" @default.
• W2019607276 hasAuthorship W2019607276A5005882758 @default.
• W2019607276 hasAuthorship W2019607276A5072355639 @default.
• W2019607276 hasAuthorship W2019607276A5074933659 @default.
• W2019607276 hasConcept C130316041 @default.
• W2019607276 hasConcept C178790620 @default.
• W2019607276 hasConcept C181199279 @default.
• W2019607276 hasConcept C185592680 @default.
• W2019607276 hasConcept C195794163 @default.
• W2019607276 hasConcept C2778078955 @default.
• W2019607276 hasConcept C2778918659 @default.
• W2019607276 hasConcept C36020004 @default.
• W2019607276 hasConcept C41625074 @default.
• W2019607276 hasConcept C519063684 @default.
• W2019607276 hasConcept C55493867 @default.
• W2019607276 hasConceptScore W2019607276C130316041 @default.
• W2019607276 hasConceptScore W2019607276C178790620 @default.
• W2019607276 hasConceptScore W2019607276C181199279 @default.
• W2019607276 hasConceptScore W2019607276C185592680 @default.
• W2019607276 hasConceptScore W2019607276C195794163 @default.
• W2019607276 hasConceptScore W2019607276C2778078955 @default.
• W2019607276 hasConceptScore W2019607276C2778918659 @default.
• W2019607276 hasConceptScore W2019607276C36020004 @default.
• W2019607276 hasConceptScore W2019607276C41625074 @default.
• W2019607276 hasConceptScore W2019607276C519063684 @default.
• W2019607276 hasConceptScore W2019607276C55493867 @default.
• W2019607276 hasIssue "2" @default.
• W2019607276 hasLocation W20196072761 @default.

• next