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- W2019631780 abstract "Chemical investigation of Codonopsis ovata resulted in the isolation and identification of β-sitosterol-3-O-glycoside, luteolin, apigenin, gentiacaulein, swertiaperenine, β-sitosterol, taraxeryl-3-acetate, and 3β-acetoxyoleanane-12-one. A rapid, precise, sensitive and validated HPTLC method for simultaneous quantification of these natural products (NPs) was developed on silica-gel 60F254 plate using ternary solvent system, n-hexane:ethyl acetate:formic acid (10.5:3.5:0.43, v/v/v). Markers were quantified after post chromatographic derivatization with cerric ammonium sulfate reagent. The method was validated for accuracy, precision, LOD, LOQ and all calibration curves showed a good linear relationship (r > 0.9924) within test range. Precision was evaluated by intra- and inter-day tests with RSDs <2.59%, accuracy validation recovery 92.43–99.50% with RSDs <1.00%. Apigenin was found major component (natural abundance: 1.103%) and β-sitosterol the least (0.0263%). The NPs displayed antioxidant activity with luteolin exhibiting maximum effect at 1 μg/mL concentration (75.9% for DPPH and 43.7% for ABTS) and others at 10 and 25 μg/mL, suggesting thereby their apparent potential use for the prevention of free radical induced diseases or as an additive element to food and pharmaceutical industry." @default.
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- W2019631780 date "2014-11-01" @default.
- W2019631780 modified "2023-10-05" @default.
- W2019631780 title "Simultaneous quantification of eight bioactive secondary metabolites from Codonopsis ovata by validated high performance thin layer chromatography and their antioxidant profile" @default.
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- W2019631780 doi "https://doi.org/10.1016/j.jpba.2014.07.034" @default.
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