Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019672562> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2019672562 abstract "Bursicon is a heterodimer neuropeptide responsible for regulating cuticle sclerotization and wing expansion in several insect species. Recent studies indicate that the action of bursicon is mediated by a specific G protein-coupled receptor DLGR2 and the cAMP/PKA signaling pathway. However, little is known regarding the genes that are regulated by bursicon. The identification of bursicon-regulated genes is the focus of this investigation.We used DNA microarray analysis to identify bursicon-regulated genes in neck-ligated flies (Drosophila melanogaster) that received recombinant bursicon (r-bursicon). Fifty four genes were found to be regulated by bursicon 1 h post r-bursicon injection, 52 being up-regulated and 2 down-regulated while 33 genes were influenced by r-bursicon 3 h post-injection (24 up-regulated and 9 down-regulated genes). Analysis of these genes by inference from the fly database http://flybase.bio.indiana.edu revealed that these genes encode proteins with diverse functions, including cell signaling, gene transcription, DNA/RNA binding, ion trafficking, proteolysis-peptidolysis, metabolism, cytoskeleton formation, immune response and cell-adhesion. Twenty eight genes randomly selected from the microarray-identified list were verified by real time PCR (qPCR) which supported the microarray data. Temporal response studies of 13 identified and verified genes by qPCR revealed that the temporal expression patterns of these genes are consistent with the microarray data.Using r-bursicon, we identified 87 genes that are regulated by bursicon, 30 of which have no previously known function. Most importantly, all genes randomly selected from the microarray-identified list were verified by real time PCR. Temporal analysis of 13 verified genes revealed that the expression of these genes was indeed induced by bursicon and correlated well with the cuticle sclerotization process. The composite data suggest that these genes play important roles in regulating the cuticle sclerotization and wing expansion processes. The data obtained here will form the basis for future studies aimed at elucidating the exact mechanisms upstream from the secretion of bursicon and its binding to target cells." @default.
- W2019672562 created "2016-06-24" @default.
- W2019672562 creator A5003055331 @default.
- W2019672562 creator A5015736102 @default.
- W2019672562 creator A5048020332 @default.
- W2019672562 creator A5057813286 @default.
- W2019672562 creator A5062395667 @default.
- W2019672562 creator A5072471830 @default.
- W2019672562 date "2008-09-18" @default.
- W2019672562 modified "2023-10-10" @default.
- W2019672562 title "Global identification of bursicon-regulated genes in Drosophila melanogaster" @default.
- W2019672562 cites W1006064478 @default.
- W2019672562 cites W1625829991 @default.
- W2019672562 cites W1902468781 @default.
- W2019672562 cites W1940955820 @default.
- W2019672562 cites W1979276944 @default.
- W2019672562 cites W1990543547 @default.
- W2019672562 cites W1993760984 @default.
- W2019672562 cites W1996150413 @default.
- W2019672562 cites W1998657138 @default.
- W2019672562 cites W2002216056 @default.
- W2019672562 cites W2013003980 @default.
- W2019672562 cites W2016399806 @default.
- W2019672562 cites W2028644023 @default.
- W2019672562 cites W2031988181 @default.
- W2019672562 cites W2041231617 @default.
- W2019672562 cites W2042725370 @default.
- W2019672562 cites W2069262704 @default.
- W2019672562 cites W2071307982 @default.
- W2019672562 cites W2076078445 @default.
- W2019672562 cites W2079675328 @default.
- W2019672562 cites W2096131346 @default.
- W2019672562 cites W2107277218 @default.
- W2019672562 cites W2135791305 @default.
- W2019672562 cites W2187699616 @default.
- W2019672562 cites W2192080449 @default.
- W2019672562 cites W2316935942 @default.
- W2019672562 doi "https://doi.org/10.1186/1471-2164-9-424" @default.
- W2019672562 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2566319" @default.
- W2019672562 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18801173" @default.
- W2019672562 hasPublicationYear "2008" @default.
- W2019672562 type Work @default.
- W2019672562 sameAs 2019672562 @default.
- W2019672562 citedByCount "25" @default.
- W2019672562 countsByYear W20196725622012 @default.
- W2019672562 countsByYear W20196725622013 @default.
- W2019672562 countsByYear W20196725622015 @default.
- W2019672562 countsByYear W20196725622016 @default.
- W2019672562 countsByYear W20196725622017 @default.
- W2019672562 countsByYear W20196725622019 @default.
- W2019672562 countsByYear W20196725622020 @default.
- W2019672562 countsByYear W20196725622021 @default.
- W2019672562 countsByYear W20196725622022 @default.
- W2019672562 crossrefType "journal-article" @default.
- W2019672562 hasAuthorship W2019672562A5003055331 @default.
- W2019672562 hasAuthorship W2019672562A5015736102 @default.
- W2019672562 hasAuthorship W2019672562A5048020332 @default.
- W2019672562 hasAuthorship W2019672562A5057813286 @default.
- W2019672562 hasAuthorship W2019672562A5062395667 @default.
- W2019672562 hasAuthorship W2019672562A5072471830 @default.
- W2019672562 hasBestOaLocation W20196725621 @default.
- W2019672562 hasConcept C104317684 @default.
- W2019672562 hasConcept C150194340 @default.
- W2019672562 hasConcept C186836561 @default.
- W2019672562 hasConcept C2780104201 @default.
- W2019672562 hasConcept C54355233 @default.
- W2019672562 hasConcept C8415881 @default.
- W2019672562 hasConcept C86803240 @default.
- W2019672562 hasConcept C95371953 @default.
- W2019672562 hasConceptScore W2019672562C104317684 @default.
- W2019672562 hasConceptScore W2019672562C150194340 @default.
- W2019672562 hasConceptScore W2019672562C186836561 @default.
- W2019672562 hasConceptScore W2019672562C2780104201 @default.
- W2019672562 hasConceptScore W2019672562C54355233 @default.
- W2019672562 hasConceptScore W2019672562C8415881 @default.
- W2019672562 hasConceptScore W2019672562C86803240 @default.
- W2019672562 hasConceptScore W2019672562C95371953 @default.
- W2019672562 hasIssue "1" @default.
- W2019672562 hasLocation W20196725621 @default.
- W2019672562 hasLocation W20196725622 @default.
- W2019672562 hasLocation W20196725623 @default.
- W2019672562 hasLocation W20196725624 @default.
- W2019672562 hasLocation W20196725625 @default.
- W2019672562 hasOpenAccess W2019672562 @default.
- W2019672562 hasPrimaryLocation W20196725621 @default.
- W2019672562 hasRelatedWork W2006024165 @default.
- W2019672562 hasRelatedWork W2053053668 @default.
- W2019672562 hasRelatedWork W2091358130 @default.
- W2019672562 hasRelatedWork W2117264420 @default.
- W2019672562 hasRelatedWork W2363311673 @default.
- W2019672562 hasRelatedWork W2392883287 @default.
- W2019672562 hasRelatedWork W2415025170 @default.
- W2019672562 hasRelatedWork W2900947575 @default.
- W2019672562 hasRelatedWork W2998765584 @default.
- W2019672562 hasRelatedWork W3032874291 @default.
- W2019672562 hasVolume "9" @default.
- W2019672562 isParatext "false" @default.
- W2019672562 isRetracted "false" @default.
- W2019672562 magId "2019672562" @default.
- W2019672562 workType "article" @default.