Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019675200> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2019675200 endingPage "66" @default.
- W2019675200 startingPage "57" @default.
- W2019675200 abstract "A protease that is activated by Ca2+ has been discovered in the alga Chara corallina. The activity of the protease was measured using the substrates succinyl-bovine serum albumin (BSA) and [14C]methyl-BSA. In the absence of CaCl2, the optimal activity of the homogenate was found to be in the acidic range. However, in the presence of CaCl2, the activity in the alkaline range increased. The concentration of CaCl2 that gave half-maximal activation of the enzyme was 100 – 500 μM at pH 7.5. The effect of Ca2+ could be partially replaced by Sr2+, but not by Mg2+. The molecular mass of the Ca2+-activated protease was estimated by gel permeation chromatography to be about 40 kD. Cell fractionation, using the vacuolar perfusion technique, showed that the enzyme is localized in the cytoplasm. The Ca2+-activation at pH 7.5 was inhibited by p-chloromercuribenzoic acid. The partially purified enzyme had negligible activity in the absence of Ca2+, showing that this enzyme has an absolute requirement for Ca2+ for its activity. This protease could be separated into two protein isoforms by activity-staining on polyacrylamide gels containing gelatin as the substrate." @default.
- W2019675200 created "2016-06-24" @default.
- W2019675200 creator A5069969681 @default.
- W2019675200 creator A5074958491 @default.
- W2019675200 date "2004-02-01" @default.
- W2019675200 modified "2023-09-23" @default.
- W2019675200 title "A novel calcium-activated protease in<i>Chara corallina</i>" @default.
- W2019675200 cites W1491294719 @default.
- W2019675200 cites W1511235377 @default.
- W2019675200 cites W1517922118 @default.
- W2019675200 cites W1533692222 @default.
- W2019675200 cites W1567844991 @default.
- W2019675200 cites W1775749144 @default.
- W2019675200 cites W1967293830 @default.
- W2019675200 cites W1976297230 @default.
- W2019675200 cites W2017532256 @default.
- W2019675200 cites W2019088865 @default.
- W2019675200 cites W2047985730 @default.
- W2019675200 cites W2053394799 @default.
- W2019675200 cites W2060495071 @default.
- W2019675200 cites W2070589373 @default.
- W2019675200 cites W2090784961 @default.
- W2019675200 cites W2108040034 @default.
- W2019675200 cites W2110232033 @default.
- W2019675200 cites W2111301002 @default.
- W2019675200 cites W2125026349 @default.
- W2019675200 cites W2132736984 @default.
- W2019675200 cites W2140692122 @default.
- W2019675200 cites W2163083851 @default.
- W2019675200 cites W2178503716 @default.
- W2019675200 cites W2235497272 @default.
- W2019675200 cites W80102945 @default.
- W2019675200 doi "https://doi.org/10.1080/69676260310001636686" @default.
- W2019675200 hasPublicationYear "2004" @default.
- W2019675200 type Work @default.
- W2019675200 sameAs 2019675200 @default.
- W2019675200 citedByCount "4" @default.
- W2019675200 countsByYear W20196752002019 @default.
- W2019675200 countsByYear W20196752002022 @default.
- W2019675200 crossrefType "journal-article" @default.
- W2019675200 hasAuthorship W2019675200A5069969681 @default.
- W2019675200 hasAuthorship W2019675200A5074958491 @default.
- W2019675200 hasBestOaLocation W20196752001 @default.
- W2019675200 hasConcept C101660505 @default.
- W2019675200 hasConcept C178790620 @default.
- W2019675200 hasConcept C181199279 @default.
- W2019675200 hasConcept C185592680 @default.
- W2019675200 hasConcept C18903297 @default.
- W2019675200 hasConcept C2775920969 @default.
- W2019675200 hasConcept C2776714187 @default.
- W2019675200 hasConcept C2777289219 @default.
- W2019675200 hasConcept C28212737 @default.
- W2019675200 hasConcept C43617362 @default.
- W2019675200 hasConcept C519063684 @default.
- W2019675200 hasConcept C55493867 @default.
- W2019675200 hasConcept C58121356 @default.
- W2019675200 hasConcept C86803240 @default.
- W2019675200 hasConcept C86807702 @default.
- W2019675200 hasConcept C96294017 @default.
- W2019675200 hasConcept C97428945 @default.
- W2019675200 hasConceptScore W2019675200C101660505 @default.
- W2019675200 hasConceptScore W2019675200C178790620 @default.
- W2019675200 hasConceptScore W2019675200C181199279 @default.
- W2019675200 hasConceptScore W2019675200C185592680 @default.
- W2019675200 hasConceptScore W2019675200C18903297 @default.
- W2019675200 hasConceptScore W2019675200C2775920969 @default.
- W2019675200 hasConceptScore W2019675200C2776714187 @default.
- W2019675200 hasConceptScore W2019675200C2777289219 @default.
- W2019675200 hasConceptScore W2019675200C28212737 @default.
- W2019675200 hasConceptScore W2019675200C43617362 @default.
- W2019675200 hasConceptScore W2019675200C519063684 @default.
- W2019675200 hasConceptScore W2019675200C55493867 @default.
- W2019675200 hasConceptScore W2019675200C58121356 @default.
- W2019675200 hasConceptScore W2019675200C86803240 @default.
- W2019675200 hasConceptScore W2019675200C86807702 @default.
- W2019675200 hasConceptScore W2019675200C96294017 @default.
- W2019675200 hasConceptScore W2019675200C97428945 @default.
- W2019675200 hasIssue "1" @default.
- W2019675200 hasLocation W20196752001 @default.
- W2019675200 hasOpenAccess W2019675200 @default.
- W2019675200 hasPrimaryLocation W20196752001 @default.
- W2019675200 hasRelatedWork W109123086 @default.
- W2019675200 hasRelatedWork W1976995328 @default.
- W2019675200 hasRelatedWork W1996003640 @default.
- W2019675200 hasRelatedWork W2016330342 @default.
- W2019675200 hasRelatedWork W2033749400 @default.
- W2019675200 hasRelatedWork W2064587910 @default.
- W2019675200 hasRelatedWork W2109078573 @default.
- W2019675200 hasRelatedWork W2109083843 @default.
- W2019675200 hasRelatedWork W2159461121 @default.
- W2019675200 hasRelatedWork W2395427371 @default.
- W2019675200 hasVolume "39" @default.
- W2019675200 isParatext "false" @default.
- W2019675200 isRetracted "false" @default.
- W2019675200 magId "2019675200" @default.
- W2019675200 workType "article" @default.