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- W2019691462 abstract "In this study, we present the difference in sterol composition of extracted steryl glycosides (SG) hydrolyzed by either enzymatic or acid hydrolysis. SG were analyzed from foods belonging to the plant families Cucurbitaceae (melon and pumpkin seeds) and Amaranthaceae (amaranth and beetroot), both of which are dominated by Δ7-sterols. Released sterols were quantified by gas chromatography with a flame ionization detector (GC-FID) and identified using gas chromatography/mass spectrometry (GC–MS). All Δ7-sterols identified (Δ7-stigmastenyl, spinasteryl, Δ7-campesteryl, Δ7-avenasteryl, poriferasta-7,25-dienyl and poriferasta-7,22,25-trienyl glucoside) underwent isomerization under acidic conditions and high temperature. Sterols with an ethylidene or methylidene side chain were found to form multiple artifacts. The artifact sterols coeluted with residues of incompletely isomerized Δ7-sterols, or Δ5-sterols if present, and could be identified as Δ8(14)-sterols on the basis of relative retention time, and their MS spectra as trimethylsilyl (TMS) and acetate derivatives. For instance, SG from melon were composed of 66 % Δ7-stigmastenol when enzymatic hydrolysis was performed, whereas with acid hydrolysis only 8 % of Δ7-stigmastenol was determined. The artifact of Δ7-stigmastenol coeluted with residual non-isomerized spinasterol, demonstrating the high risk of misinterpretation of compositional data obtained after acid hydrolysis. Therefore, the accurate composition of SG from foods containing sterols with a double bond at C-7 can only be obtained by enzymatic hydrolysis or by direct analysis of the intact SG." @default.
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- W2019691462 date "2015-03-11" @default.
- W2019691462 modified "2023-10-03" @default.
- W2019691462 title "Comparison of Enzymatic Hydrolysis and Acid Hydrolysis of Sterol Glycosides from Foods Rich in Δ7-Sterols" @default.
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- W2019691462 doi "https://doi.org/10.1007/s11745-015-4002-3" @default.
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