Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019698047> ?p ?o ?g. }
- W2019698047 endingPage "e36188" @default.
- W2019698047 startingPage "e36188" @default.
- W2019698047 abstract "We have investigated the in vitro effects of increased levels of glucose and free fatty acids on autophagy activation in pancreatic beta cells. INS-1E cells and isolated rat and human pancreatic islets were incubated for various times (from 2 to 24 h) at different concentrations of glucose and/or palmitic acid. Then, cell survival was evaluated and autophagy activation was explored by using various biochemical and morphological techniques. In INS-1E cells as well as in rat and human islets, 0.5 and 1.0 mM palmitate markedly increased autophagic vacuole formation, whereas high glucose was ineffective alone and caused little additional change when combined with palmitate. Furthermore, LC3-II immunofluorescence co-localized with that of cathepsin D, a lysosomal marker, showing that the autophagic flux was not hampered in PA-treated cells. These effects were maintained up to 18–24 h incubation and were associated with a significant decline of cell survival correlated with both palmitate concentration and incubation time. Ultrastructural analysis showed that autophagy activation, as evidenced by the occurrence of many autophagic vacuoles in the cytoplasm of beta cells, was associated with a diffuse and remarkable swelling of the endoplasmic reticulum. Our results indicate that among the metabolic alterations typically associated with type 2 diabetes, high free fatty acids levels could play a role in the activation of autophagy in beta cells, through a mechanism that might involve the induction of endoplasmic reticulum stress." @default.
- W2019698047 created "2016-06-24" @default.
- W2019698047 creator A5000550995 @default.
- W2019698047 creator A5004928192 @default.
- W2019698047 creator A5018890288 @default.
- W2019698047 creator A5035837693 @default.
- W2019698047 creator A5038355565 @default.
- W2019698047 creator A5048913575 @default.
- W2019698047 creator A5054574491 @default.
- W2019698047 creator A5064940370 @default.
- W2019698047 creator A5087771312 @default.
- W2019698047 date "2012-05-01" @default.
- W2019698047 modified "2023-10-11" @default.
- W2019698047 title "Palmitate Activates Autophagy in INS-1E β-Cells and in Isolated Rat and Human Pancreatic Islets" @default.
- W2019698047 cites W1598254642 @default.
- W2019698047 cites W1969616819 @default.
- W2019698047 cites W1970420856 @default.
- W2019698047 cites W1971713572 @default.
- W2019698047 cites W1973135736 @default.
- W2019698047 cites W1977352903 @default.
- W2019698047 cites W1977767269 @default.
- W2019698047 cites W1989051519 @default.
- W2019698047 cites W1990045305 @default.
- W2019698047 cites W1992659717 @default.
- W2019698047 cites W1993446047 @default.
- W2019698047 cites W1994823455 @default.
- W2019698047 cites W2004325124 @default.
- W2019698047 cites W2005727437 @default.
- W2019698047 cites W2007505836 @default.
- W2019698047 cites W2009032039 @default.
- W2019698047 cites W2009717329 @default.
- W2019698047 cites W2011580247 @default.
- W2019698047 cites W2013705864 @default.
- W2019698047 cites W2014506057 @default.
- W2019698047 cites W2020468088 @default.
- W2019698047 cites W2022570265 @default.
- W2019698047 cites W2024992738 @default.
- W2019698047 cites W2030287946 @default.
- W2019698047 cites W2032045122 @default.
- W2019698047 cites W2033627840 @default.
- W2019698047 cites W2034218807 @default.
- W2019698047 cites W2039607936 @default.
- W2019698047 cites W2044241702 @default.
- W2019698047 cites W2059188329 @default.
- W2019698047 cites W2059773828 @default.
- W2019698047 cites W2062707784 @default.
- W2019698047 cites W2067692987 @default.
- W2019698047 cites W2068986514 @default.
- W2019698047 cites W2074369108 @default.
- W2019698047 cites W2074549465 @default.
- W2019698047 cites W2077269293 @default.
- W2019698047 cites W2078911476 @default.
- W2019698047 cites W2079569342 @default.
- W2019698047 cites W2085822109 @default.
- W2019698047 cites W2089560759 @default.
- W2019698047 cites W2092999710 @default.
- W2019698047 cites W2093908424 @default.
- W2019698047 cites W2097212778 @default.
- W2019698047 cites W2105043890 @default.
- W2019698047 cites W2105497606 @default.
- W2019698047 cites W2108709856 @default.
- W2019698047 cites W2109034822 @default.
- W2019698047 cites W2117875646 @default.
- W2019698047 cites W2119713108 @default.
- W2019698047 cites W2120653342 @default.
- W2019698047 cites W2122682342 @default.
- W2019698047 cites W2125738477 @default.
- W2019698047 cites W2126120833 @default.
- W2019698047 cites W2126610675 @default.
- W2019698047 cites W2126937565 @default.
- W2019698047 cites W2127763247 @default.
- W2019698047 cites W2127916470 @default.
- W2019698047 cites W2135574023 @default.
- W2019698047 cites W2139941678 @default.
- W2019698047 cites W2140049768 @default.
- W2019698047 cites W2145206598 @default.
- W2019698047 cites W2145869245 @default.
- W2019698047 cites W2156204225 @default.
- W2019698047 cites W2159754384 @default.
- W2019698047 cites W2168212241 @default.
- W2019698047 cites W2171690812 @default.
- W2019698047 doi "https://doi.org/10.1371/journal.pone.0036188" @default.
- W2019698047 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3341371" @default.
- W2019698047 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22563482" @default.
- W2019698047 hasPublicationYear "2012" @default.
- W2019698047 type Work @default.
- W2019698047 sameAs 2019698047 @default.
- W2019698047 citedByCount "109" @default.
- W2019698047 countsByYear W20196980472012 @default.
- W2019698047 countsByYear W20196980472013 @default.
- W2019698047 countsByYear W20196980472014 @default.
- W2019698047 countsByYear W20196980472015 @default.
- W2019698047 countsByYear W20196980472016 @default.
- W2019698047 countsByYear W20196980472017 @default.
- W2019698047 countsByYear W20196980472018 @default.
- W2019698047 countsByYear W20196980472019 @default.
- W2019698047 countsByYear W20196980472020 @default.
- W2019698047 countsByYear W20196980472021 @default.